Anti-beta Synuclein抗体(ab6165)
Key features and details
- Rabbit polyclonal to beta Synuclein
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-beta Synuclein抗体
参阅全部 beta Synuclein 一抗 -
描述
兔多克隆抗体to beta Synuclein -
宿主
Rabbit -
特异性
Less than 0.1% cross reactivity against human alpha-synuclein Cross reactivity: This antibody is known to react with human and rat. Not yet tested in other species. -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide (IEPLMEPEGSYEDPPQE) corresponding to the carboxyl terminal of human beta-synuclein (108- 125) and was conjugated to diphtheria toxoid via a cystine residue added to the carboxyl terminus of the peptide. The conjugate was cross-linked by maleimidocaproyl-N-hydroxy succinimide.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50% for extra stability and aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7 -
Concentration information loading... -
纯度
Whole antiserum -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Recombinant Protein
应用
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (1) |
1/500 - 1/2000.
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| 说明 |
|---|
|
IHC-P
1/500 - 1/2000. |
靶标
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功能
Non-amyloid component of senile plaques found in Alzheimer disease. Could act as a regulator of SNCA aggregation process. Protects neurons from staurosporine and 6-hydroxy dopamine (6OHDA)-stimulated caspase activation in a p53/TP53-dependent manner. Contributes to restore the SNCA anti-apoptotic function abolished by 6OHDA. Not found in the Lewy bodies associated with Parkinson disease. -
组织特异性
Expressed predominantly in brain; concentrated in presynaptic nerve terminals. -
序列相似性
Belongs to the synuclein family. -
翻译后修饰
Phosphorylated. Phosphorylation by G-protein coupled receptor kinases (GRK) is more efficient than phosphorylation by CK1, CK2 and CaM-kinase II. -
细胞定位
Cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 6620 Human
- Omim: 602569 Human
- SwissProt: Q16143 Human
- Unigene: 90297 Human
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别名
- 14 kDa brain-specific protein antibody
- Beta-synuclein antibody
- Phosphoneuroprotein 14 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Synuclein antibody (ab6165)ab6165 staining beta synuclein in human occipital cortex.
Left panel: with primary antibody diluted 1:4000. Right panel: isotype control.
Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
实验方案
数据表及文件
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Datasheet download
文献 (7)
ab6165 被引用在 7 文献中.
- Klinkenberg M et al. Interneuronal In Vivo Transfer of Synaptic Proteins. Cells 12:N/A (2023). PubMed: 36831238
- Lahut S et al. Blood RNA biomarkers in prodromal PARK4 and rapid eye movement sleep behavior disorder show role of complexin 1 loss for risk of Parkinson's disease. Dis Model Mech 10:619-631 (2017). PubMed: 28108469
- Helwig M et al. Brain propagation of transduced a-synuclein involves non-fibrillar protein species and is enhanced in a-synuclein null mice. Brain 139:856-70 (2016). WB ; Mouse . PubMed: 26719384
- Prabhudesai S et al. LRRK2 knockdown in zebrafish causes developmental defects, neuronal loss, and synuclein aggregation. J Neurosci Res 94:717-35 (2016). PubMed: 27265751
- Vilas D et al. Pick's pathology in Parkinson's disease with dementia. Neuropathol Appl Neurobiol 38:737-43 (2012). PubMed: 22686686
- Beyer K et al. The decrease of {beta}-synuclein in cortical brain areas defines a molecular subgroup of dementia with Lewy bodies. Brain : (2010). WB ; Human . PubMed: 20959308
- Iwata A et al. alpha-Synuclein affects the MAPK pathway and accelerates cell death. J Biol Chem 276:45320-9 (2001). WB ; Human . PubMed: 11560921
