重组beta IV Tubulin抗体[EPR16775](ab179504)|Abcam

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Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16775] to beta IV Tubulin
Suitable for: IHC-P, WB, ICC/IF, Flow Cyt (Intra)
Reacts with: Mouse, Rat, Human

Alexa Fluor® 488 Alexa Fluor® 555 Alexa Fluor® 568 Alexa Fluor® 594 Alexa Fluor® 647 Alexa Fluor® 750 Alkaline Phosphatase APC Carrier Free HRP PE

产品名称

Anti-beta IV Tubulin抗体[EPR16775]
参阅全部 beta IV Tubulin 一抗
描述

兔单克隆抗体[EPR16775] to beta IV Tubulin
宿主

Rabbit
经测试应用

适用于: IHC-P, WB, ICC/IF, Flow Cyt (Intra)more details
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: Human fetal brain, Human cerebellum and Human fetal kidney lysates; HeLa, Jurkat, Neuro-2a, C6, RAW 264.7, and PC-12 whole cell lysates; Mouse brain, heart, kidney and spleen lysates; Rat brain and heart lysates. IHC-P: Human cerebral cortex, Mouse hippocampus and Rat thalamus tissues. ICC/IF: HeLa cells.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR16775
同种型

IgG
研究领域

The Abpromise guarantee

Abpromise™承诺保证使用ab179504于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
IHC-P		1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC/IF		1/100.
Flow Cyt (Intra)		1/20.

说明
IHC-P 1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/5000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
ICC/IF 1/100.
Flow Cyt (Intra) 1/20.

功能

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
序列相似性

Belongs to the tubulin family.
结构域

The highly acidic C-terminal region may bind cations such as calcium.
翻译后修饰

Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
细胞定位

Cytoplasm > cytoskeleton.
Target information above from: UniProt accession P04350 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 10382 Human
- Entrez Gene: 22153 Mouse
- Entrez Gene: 29213 Rat
- Omim: 602662 Human
- SwissProt: P04350 Human
- SwissProt: Q9D6F9 Mouse
- Unigene: 110837 Human
- Unigene: 7420 Mouse
别名
- Beta 4 antibody
- Beta 4 tubulin antibody
- beta 5 antibody
- beta four tubulin antibody
- Dystonia 4 torsion (autosomal dominant) antibody
- MC1R antibody
- TBB4_HUMAN antibody
- TUB B4 antibody
- TUBB 4 antibody
- tubb4 antibody
- TUBB4A antibody
- TUBB5 antibody
- Tubulin 5 beta antibody
- Tubulin beta 3 antibody
- Tubulin beta 4 antibody
- Tubulin beta 4 chain antibody
- Tubulin beta 4A class IVa antibody
- Tubulin beta 5 antibody
- Tubulin beta IV antibody
- Tubulin beta-4 chain antibody
see all

Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)

All lanes : Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution

Lane 1 : Human fetal brain lysates
Lane 2 : Human cerebellum lysates
Lane 3 : Human fetal kidney lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/dilution buffer: 5% NFDM/TBST.
Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)

All lanes : Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution

Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
Lane 3 : Neuro-2a (Mouse neuroblastoma cells) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/dilution buffer: 5% NFDM/TBST.
Western blot - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)

All lanes : Anti-beta IV Tubulin antibody [EPR16775] (ab179504) at 1/5000 dilution

Lane 1 : Mouse brain lysates
Lane 2 : Mouse heart lysates
Lane 3 : Mouse kidney lysates
Lane 4 : Mouse spleen lysates
Lane 5 : Rat brain lysates
Lane 6 : Rat heart lysates
Lane 7 : Rat kidney lysates
Lane 8 : Rat spleen lysates
Lane 9 : C6 (Rat glial tumor cells) whole cell lysates
Lane 10 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 11 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 12 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% tritonX-100 permeabilized HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling beta IV Tubulin with ab179504 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody (ab150077) at 1/200 dilution (green). Cytoplasm staining is observed.
Tubulin is detected with Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/400 dilution (red). The nuclear counter stain is DAPI (blue).

The negative controls:-
-ve control 1 - ab179504 at 1/100 dilution followed by Goat anti mouse IgG (Alexa Fluor®594) at 1/400 dilution.
-ve control 2 - Mouse anti-Tubulin antibody (ab7291) at 1/500 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) at 1/200 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)

Immunohistochemical analysis of paraffin-embedded Human cerebral cortex issue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of cerebral cortex.

Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)

Immunohistochemical analysis of paraffin embedded Mouse hippocampus tissue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of mouse hippocampus.

Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)

Immunohistochemical analysis of paraffin-embedded Rat thalamus tissue labeling beta IV Tubulin with ab179504 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with Hematoxylin. Cytoplasm staining is observed on neurons of rat thalamus.

Negative control used PBS instead of ab179504, followed by prediluted HRP Polymer for Rabbit/Mouse IgG.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-beta IV Tubulin antibody [EPR16775] (ab179504)

Intracellular Flow Cytometry analysis of 2% paraformaldehyde fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling beta IV Tubulinwith ab179504 at 1/20 dilution (red line). Secondary antibody used is a goat anti rabbit IgG (FITC) at 1/150 dilution. The isotype control is rabbit monoclonal IgG (black line).
Anti-beta IV Tubulin antibody [EPR16775] (ab179504)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

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Datasheet download

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发表研究结果有使用 ab179504？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab179504 被引用在 10 文献中.

Di Silvestre D et al. The Protein Network in Subcutaneous Fat Biopsies from Patients with AL Amyloidosis: More Than Diagnosis? Cells 12:N/A (2023). PubMed: 36899835
Essaidi-Laziosi M et al. Sequential infections with rhinovirus and influenza modulate the replicative capacity of SARS-CoV-2 in the upper respiratory tract. Emerg Microbes Infect 11:412-423 (2022). PubMed: 34931581
Wang J et al. SARS-CoV-2 uses metabotropic glutamate receptor subtype 2 as an internalization factor to infect cells. Cell Discov 7:119 (2021). PubMed: 34903715
Hausrat TJ et al. Alpha- and beta-tubulin isotypes are differentially expressed during brain development. Dev Neurobiol 81:333-350 (2021). PubMed: 32293117
Yuan T et al. Abnormal Expression of YAP Is Associated With Proliferation, Differentiation, Neutrophil Infiltration, and Adverse Outcome in Patients With Nasal Inverted Papilloma. Front Cell Dev Biol 9:625251 (2021). PubMed: 33937228
Sun SH et al. A Mouse Model of SARS-CoV-2 Infection and Pathogenesis. Cell Host Microbe 28:124-133.e4 (2020). PubMed: 32485164
Zhu N et al. Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells. Nat Commun 11:3910 (2020). PubMed: 32764693
Bi B et al. Quantitative Proteomic Analysis Reveals Impaired Axonal Guidance Signaling in Human Postmortem Brain Tissues of Chronic Traumatic Encephalopathy. Exp Neurobiol 28:362-375 (2019). PubMed: 31308796
Royston L et al. Viral chimeras decrypt the role of enterovirus capsid proteins in viral tropism, acid sensitivity and optimal growth temperature. PLoS Pathog 14:e1006962 (2018). PubMed: 29630666
Essaidi-Laziosi M et al. Propagation of respiratory viruses in human airway epithelia reveals persistent virus-specific signatures. J Allergy Clin Immunol 141:2074-2084 (2018). ICC/IF . PubMed: 28797733

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Key features and details

Related conjugates and formulations

概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Isotype control

应用

The Abpromise guarantee

靶标

功能

序列相似性

结构域

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

文献 (10)

客户评价及客户问答