重组Anti-beta II Tubulin抗体[7B9]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody [7B9] (AB28035)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized U-87 MG cells labelling beta II Tubulin with ab28035 at 1/50 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in U-87 MG cell line. ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab28035 at a 1/500 dilution followed by ab150080 at a 1/500 dilution.

Negative control 2 : ab179513 at a 1/500 dilution followed by ab150113 at a 1/1000 dilution.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody [7B9] (AB28035)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Neuro-2a cells labelling beta II Tubulin with ab28035 at 1/50 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in Neuro-2a cell line. ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab28035 at a 1/500 dilution followed by ab150080 at a 1/500 dilution.

Negative control 2 : ab179513 at a 1/500 dilution followed by ab150113 at a 1/1000 dilution.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-beta II Tubulin antibody [7B9] (AB28035)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized PC-12 cells labelling beta II Tubulin with ab28035 at 1/50 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in PC-12 cell line. ab179513 anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/500 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) (Red). The nuclear counterstain was DAPI (Blue).

Negative control 1 : ab28035 at a 1/500 dilution followed by ab150080 at a 1/500 dilution.

Negative control 2 : ab179513 at a 1/500 dilution followed by ab150113 at a 1/1000 dilution.

• IP

Unknown

Immunoprecipitation - Anti-beta II Tubulin antibody [7B9] (AB28035)

beta II Tubulin was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate with ab28035 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab28035 at 1/1000 dilution. mouse IgG for IP (HRP) (ab131368) was used at 1/1000 dilution.

Lane 1 : Neuro-2a whole cell lysate 10μg.

Lane 2 : ab28035 IP in Neuro-2a whole cell lysate.

Lane 3 : Mouse monoclonal IgG (ab18443) instead of ab28035 in Neuro-2a whole cell lysate.

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : 3 seconds.

All lanes:

Immunoprecipitation - Anti-beta II Tubulin antibody [7B9] (ab28035)

Predicted band size: 50 kDa

false

• WB

Lab

Western blot - Anti-beta II Tubulin antibody [7B9] (AB28035)

Blocking/Dilution buffer : 5% NFDM/TBST.

Negative control : Human heart (PMID : 20191564).

All lanes:

Western blot - Anti-beta II Tubulin antibody [7B9] (ab28035) at 1/1000 dilution

Lane 1:

Human brain tissue lysate at 20 µg

Lane 2:

Human heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 50 kDa

false

Exposure time: 1s

• WB

Lab

Western blot - Anti-beta II Tubulin antibody [7B9] (AB28035)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-beta II Tubulin antibody [7B9] (ab28035) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

Y79 (human retinoblastoma retinoblastoma), whole cell lysate at 20 µg

Lane 3:

MDA-MB-231 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/1000 dilution

Predicted band size: 50 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-beta II Tubulin antibody [7B9] (AB28035)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : lane 1, 2 : 1 second
lane 3 : 3 seconds

All lanes:

Western blot - Anti-beta II Tubulin antibody [7B9] (ab28035) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Lane 2:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Predicted band size: 50 kDa

false

• WB

Lab

Western blot - Anti-beta II Tubulin antibody [7B9] (AB28035)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-beta II Tubulin antibody [7B9] (ab28035) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Rat brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Anti-mouse IgG for IP (HRP) (<a href='/products/secondary-antibodies/mouse-igg-for-ip-hrp-ab131368'>ab131368</a>) at 1/1000 dilution

Predicted band size: 50 kDa

false

Exposure time: 1s