重组beta I Tubulin抗体[EPR16778](ab179511)|Abcam中文官网

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Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR16778] to beta I Tubulin
Suitable for: Flow Cyt (Intra), ICC/IF, IHC-P, WB
Reacts with: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, African green monkey

Alexa Fluor® 488 Carrier Free

产品名称

Anti-beta I Tubulin抗体[EPR16778]
参阅全部 beta I Tubulin 一抗
描述

兔单克隆抗体[EPR16778] to beta I Tubulin
宿主

Rabbit
经测试应用

适用于: Flow Cyt (Intra), ICC/IF, IHC-P, WBmore details
种属反应性

与反应: Mouse, Rat, Chicken, Hamster, Cow, Dog, Human, African green monkey
预测可用于: Monkey
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: Human fetal brain, fetal heart and fetal kidney lysates; Mouse brain, Rat brain and Rat spleen lysates; K562, Jurkat, HeLa, 293T, C6, RAW 264.7, PC-12, NIH/3T3, UMNSAH/DF-1, BHK, MDCK, MDBK, and COS-1 whole cell lysates. IHC-P: Human tonsil tissue; Mouse cerebral cortex tissue; Rat spleen tissue. ICC/IF: K562 cells. Flow Cyt (intra): K562 cells.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存储溶液

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR16778
同种型

IgG
研究领域

Alternative Versions
- Alexa Fluor® 488 Anti-beta I Tubulin antibody [EPR16778] (ab201831)
- Anti-beta I Tubulin antibody [EPR16778] - BSA and Azide free (ab250104)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human beta I Tubulin protein (ab164310)

The Abpromise guarantee

Abpromise™承诺保证使用ab179511于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
Flow Cyt (Intra)		1/80. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF		1/500.
IHC-P		1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB		1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

说明
Flow Cyt (Intra) 1/80. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/500.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).

功能

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
疾病相关

Defects in TUBB1 are a cause of macrothrombocytopenia autosomal dominant TUBB1-related (MAD-TUBB1) [MIM:613112]. It is a congenital blood disorder characterized by increased platelet size and decreased number of circulating platelets.
序列相似性

Belongs to the tubulin family.
翻译后修饰

Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules.
细胞定位

Cytoplasm > cytoskeleton.
Target information above from: UniProt accession Q9H4B7 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 103243432 African green monkey
- Entrez Gene: 396427 Chicken
- Entrez Gene: 541271 Cow
- Entrez Gene: 485950 Dog
- Entrez Gene: 101836899 Hamster
- Entrez Gene: 81027 Human
- Entrez Gene: 545486 Mouse
- Entrez Gene: 679312 Rat
- Omim: 612901 Human
- SwissProt: P09203 Chicken
- SwissProt: Q9H4B7 Human
- SwissProt: A2AQ07 Mouse
- Unigene: 303023 Human
see all
别名
- 2810484G07Rik antibody
- beta 1 tubulin antibody
- Beta tubulin 1, class VI antibody
- Beta-tubulin antibody
- Class VI beta tubulin antibody
- dJ543J19.4 antibody
- M(beta)1 antibody
- TBB1_HUMAN antibody
- TUBB1 antibody
- Tubulin beta 1 class VI antibody
- Tubulin beta-1 chain antibody
- Tubulin, beta 1 antibody
- tubulin, beta1 antibody
see all

Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/20000 dilution

Lane 1 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysates
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysates
Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysates
Lane 4 : 293T (Human epithelial cells from embryonic kidney) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution

Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal heart lysate
Lane 3 : Human fetal kidney lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Rat brain lysate
Lane 3 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/2000 dilution

Lane 1 : C6 (Rat glial tumor cells) whole cell lysates
Lane 2 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysates
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysates
Lane 4 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Western blot - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

All lanes : Anti-beta I Tubulin antibody [EPR16778] (ab179511) at 1/1000 dilution

Lane 1 : UMNSAH/DF-1 (Transformed chicken embyronic fibroblast cells) whole cell lysates
Lane 2 : BHK (Hamster kidney fibroblast cells) whole cell lysates
Lane 3 : MDCK (Canine kidney cell line) whole cell lysates
Lane 4 : MDBK (Bovine kidney cell line) whole cell lysates
Lane 5 : COS-1 (African green monkey kidney fibroblast-like cell line) whole cell lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated antibody at 1/1000 dilution

Predicted band size: 50 kDa
Observed band size: 50 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling beta I Tubulin with ab179511 at 1/500 dilution followed by AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/400 dilution (green). Cytoplasm and nuclear staining on K562 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution (red).
The negative controls are as follows;
1. ab179511 at 1/500 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/500 dilution.
2. ab7291 (anti-Tubulin mouse mAb) at 1/500 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/400 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on germinal center lymphocytes of Human tonsil is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

Immunohistochemical analysis of paraffin-embedded Mouse cerebral cortex tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on neuron cells of Mouse cerebral cortex tissue is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling beta I Tubulin with ab179511 at 1/500 followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Cytoplasm staining on follicular center lymphocytes of Rat spleen tissue is observed. Counter stained with Hematoxylin.

Negative control: Using PBS instead of primary ab, secondary ab is prediluted HRP Polymer for Rabbit/Mouse IgG.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-beta I Tubulin antibody [EPR16778] (ab179511)

Intracellular flow cytometric analysis of2% paraformaldehyde-fixed K562 (Human chronic myelogenous leukemia cells from bone marrow) cells labeling beta I Tubulin with ab179511 at 1/80 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.
Anti-beta I Tubulin antibody [EPR16778] (ab179511)

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

SDS download

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Datasheet download

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ab179511 被引用在 11 文献中.

Xin L et al. Honokiol alleviates radiation-induced premature ovarian failure via enhancing Nrf2. Am J Reprod Immunol 90:e13769 (2023). PubMed: 37766410
Wang R et al. Autophagy-mediated NKG2D internalization impairs NK cell function and exacerbates radiation pneumonitis. Front Immunol 14:1250920 (2023). PubMed: 38077388
Rosano S et al. Long Non-Coding RNA LINC02802 Regulates In Vitro Sprouting Angiogenesis by Sponging microRNA-486-5p. Int J Mol Sci 23:N/A (2022). PubMed: 35163581
Peng D et al. Ascorbic acid induced TET2 enzyme activation enhances cancer immunotherapy efficacy in renal cell carcinoma. Int J Biol Sci 18:995-1007 (2022). PubMed: 35173532
Chen P et al. A plant-derived natural photosynthetic system for improving cell anabolism. Nature 612:546-554 (2022). PubMed: 36477541

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Key features and details

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概述

产品名称

描述

宿主

经测试应用

种属反应性

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

疾病相关

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

Certificate of Compliance

文献 (11)

客户评价及客户问答