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AB116

Anti-beta Galactosidase抗体[DC1 4C7]

Anti-beta Galactosidase antibody [DC1 4C7]

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(8 Publications)

Mouse Monoclonal GLB1/Beta-galactosidase antibody. Suitable for WB, ELISA and reacts with Escherichia coli samples. Cited in 8 publications.

查看别名

b0344, JW0335, lacZ, Beta-galactosidase, Beta-gal, Lactase

1 Images
Western blot - Anti-beta Galactosidase antibody [DC1 4C7] (AB116)
  • WB

Lab

Western blot - Anti-beta Galactosidase antibody [DC1 4C7] (AB116)

All lanes:

Western blot - Anti-beta Galactosidase antibody [DC1 4C7] (ab116) at 1 µg

Lane 1:

Western blot - Recombinant Human GLB1/Beta-galactosidase protein (<a href='/products/proteins-peptides/recombinant-human-glb1-beta-galactosidase-protein-ab151890'>ab151890</a>) at 0.1 µg

Lane 2:

Western blot - Native E. coli beta Galactosidase protein (<a href='/products/proteins-peptides/native-e-coli-beta-galactosidase-protein-ab79449'>ab79449</a>) at 0.1 µg

Secondary

All lanes:

Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) (ab65485) at 1/5000 dilution

Predicted band size: 116 kDa,118 kDa,183 kDa,43 kDa,54 kDa,56 kDa,72 kDa,74 kDa,84 kDa

false

Exposure time: 4min

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

DC1 4C7

亚型

IgG1

轻链类型

kappa

不含载体蛋白

No

反应种属

Escherichia coli

应用

WB, ELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Escherichia coli": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>" } } }

产品详情

This monoclonal is also known as clone HD4C7 or HD 447C.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

性能和储存信息

形式
Liquid
纯化工艺
Affinity purification
存储溶液
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 6.97% L-Arginine
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Beta Galactosidase often called "β-gal" "b-galactosidase" or "b-gal" serves as an important enzyme that hydrolyzes β-galactosides into monosaccharides. It breaks down lactose into glucose and galactose which are easier to absorb. Beta-galactosidase expresses in many organisms such as E. coli where it is a well-known component of the lac operon. Its molecular weight in E. coli is approximately 116 kDa. The enzyme also appears in human tissues but is synthesized and regulated differently compared to bacterial systems.
Biological function summary

This enzyme is involved in the metabolism of galactose a critical aspect of cellular energy production. It can participate in various metabolic pathways converting complex carbohydrates into simpler sugars. In bacteria beta-galactosidase belongs to a protein complex controlled by the lac operon regulating lactose metabolism. In humans the enzyme takes part in lysosomal functions contributing to the degradation of glycoproteins and glycolipids therefore supporting normal cellular processes.

Pathways

Beta-galactosidase plays an important role in the lactose metabolism pathway. In E. coli it coheres with the enzymes of the lac system like lactose permease and transacetylase to facilitate lactose utilization. In human biology it participates in the lysosomal degradation pathway. Its function is associated with proteins such as galactosylceramidase which also break down galactolipids demonstrating its interaction in broader metabolic networks.

Deficiencies or malfunctions in beta-galactosidase lead to metabolic conditions like Galactosialidosis and Morquio syndrome Type B. These are lysosomal storage disorders caused by a lack of enzyme activity leading to accumulation of partially degraded macromolecules. The enzyme's impaired function connects with other proteins such as protective protein cathepsin A in the case of Galactosialidosis causing complex clinical symptoms through disrupted protein interactions and pathways.

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

See full target information lacZ

其他靶点

beta Galactosidase

文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:813 PubMed38071330

2023

Lysosomes, caspase-mediated apoptosis, and cytoplasmic activation of P21, but not cell senescence, participate in a redundant fashion in embryonic morphogenetic cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Cristina Duarte-Olivenza,Goretti Moran,Juan M Hurle,Carlos I Lorda-Diez,Juan A Montero

Nature communications 12:6941 PubMed34862367

2021

Inhibition of cytoplasmic EZH2 induces antitumor activity through stabilization of the DLC1 tumor suppressor protein.

Applications

Unspecified application

Species

Unspecified reactive species

Brajendra K Tripathi,Meghan F Anderman,Disha Bhargava,Luciarita Boccuzzi,Xiaolan Qian,Dunrui Wang,Marian E Durkin,Alex G Papageorge,Fernando J de Miguel,Katerina Politi,Kylie J Walters,James H Doroshow,Douglas R Lowy

Nature 523:329-32 PubMed26131929

2015

Nanotubes mediate niche-stem-cell signalling in the Drosophila testis.

Applications

Unspecified application

Species

Unspecified reactive species

Mayu Inaba,Michael Buszczak,Yukiko M Yamashita

PloS one 7:e52335 PubMed23272238

2012

Recapitulation of tumor heterogeneity and molecular signatures in a 3D brain cancer model with decreased sensitivity to histone deacetylase inhibition.

Applications

IHC-P

Species

Unspecified reactive species

Stuart J Smith,Martin Wilson,Jennifer H Ward,Cheryl V Rahman,Andrew C Peet,Donald C Macarthur,Felicity R A J Rose,Richard G Grundy,Ruman Rahman

Arteriosclerosis, thrombosis, and vascular biology 30:1423-9 PubMed20431063

2010

Repressors NFI and NFY participate in organ-specific regulation of von Willebrand factor promoter activity in transgenic mice.

Applications

IHC-Fr

Species

Unspecified reactive species

Marjan Nassiri,Ju Liu,Stephen Kulak,Richard R E Uwiera,William C Aird,Barbara J Ballermann,Nadia Jahroudi

Developmental biology 330:329-38 PubMed19361496

2009

Multiple lineage-specific roles of Smad4 during neural crest development.

Applications

Unspecified application

Species

Unspecified reactive species

Stine Büchmann-Møller,Iris Miescher,Nessy John,Jaya Krishnan,Chu-Xia Deng,Lukas Sommer

The Journal of biological chemistry 283:2741-50 PubMed18048367

2007

Sequences in intron 51 of the von Willebrand factor gene target promoter activation to a subset of lung endothelial cells in transgenic mice.

Applications

IHC-Fr

Species

Unspecified reactive species

Ann M Kleinschmidt,Marjan Nassiri,Molly S Stitt,Karla Wasserloos,Simon C Watkins,Bruce R Pitt,Nadia Jahroudi

Genesis (New York, N.Y. : 2000) 41:73-80 PubMed15682388

2005

Rhodopsin-iCre transgenic mouse line for Cre-mediated rod-specific gene targeting.

Applications

IHC

Species

Unspecified reactive species

Sha Li,Desheng Chen,Yves Sauvé,Jeremy McCandless,Yu-Jiun Chen,Ching-Kang Chen
View all publications

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