Anti-beta Galactosidase抗体(ab4761)

Code de remise : xxxxxx
Date d’expiration : xxxxxxxx
Valeur: €xxxxxx

Je suis ravie que vous souhaitiez participer a notre offre et tester ab4761 chez la souris. Le code de réduction est xxxxxxxxxx. Ce code de réduction est utilisable sous 120 jours et pour l’achat d’un autre produit.

Premièrement pour passer la commande vous devez soit téléphoner à notre service clientèle sur : 01.46.94.62.96 ou envoyer votre commande par Email à mailto:orders@abcam.com ou par Fax au 01.70.80.96.08.

Une fois que vous avez testé l’anticorps, vous devez soumettre une Abreview avec vos résultats chez la souris en notant ce code dans la section « Additional notes ». Ce code sera ainsi activé et vous pourrez par la suite contacter notre service clientèle par téléphone pour passer votre prochaine commande, en vous munissant des informations suivantes : code de réduction, numéro de commande de ab4761.

Nous restons à votre disposition pour plus d’informations concernant cette offre promotionnelle. Dans l’attente de recevoir votre Abreview nous vous souhaitons bonne chance dans vos recherches.

Termes et Conditions : www.abcam.com/collaborationdiscount.

Merci de nous avoir contactés.

L’anticorps ab4761 a été testé en IHC-P et WB. L’espèce testée en IHC-P est l’humain.

Voir la publication suivante : Hachiya A et al. Gene transfer in human skin with different pseudotyped HIV-based vectors. Gene Ther 14:648-56 (2007). Pour en lire plus allez voir sur PubMed: 17268532

L’anticorps a aussi été testé en WB avec des lysâtes de cellules Hela.

Si vous ne préférez pas essayer cet anticorps chez la souris et de participer à notre offre de test, vous pouvez essayer l’anticorps ab9361 (www.abcam.com/ab9361) qui lui a été testé chez la souris en IHC-P et est couvert par notre Garantie Abpromise.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Merci de nous avoir contactés.

Nous n'avons pas encore testé l’anticorps ab4761 chez la souris. Cependant, puisque nous n'avons pour l'instant pas d'image IHC-P chez la souris à mettre sur la fiche technique du ab4761 il me sera possible de vous offrir une remise si vous acceptez de tester ab4761 chez la souris et de partager vos résultats. Après avoir soumis vos résultats sous forme d'Abreview, un avoir de la valeur de l'anticorps ab4761 sera alors crédité sur votre compte client.

Étapes à suivre :

1. Nous informer que vous souhaitez tester l’anticorps chez la souris et nous vous enverrons un code promotionnel (valable 4 mois) à utiliser après obtention et envoi de vos résultats,

2. Acheter l'anticorps,

3. Nous envoyer vos résultats (positifs ou négatifs) obtenus sous forme d'Abreview (www.abcam.com/abreviews).

4. L'envoi de vos résultats activera le code et vous donnera un avoir de la valeur de l'anticorps testé.

N’hésitez pas à nous demander plus d’informations concernant cette offre promotionnelle.

Sinon, je peux vous proposer d’utiliser un autre anticorps ab9361 (https://www.abcam.com/ab9361) qui a été testé sur coupes de souris fixées Formalin en IHC-P et est couvert par notre Garantie Abpromise. Notre politique est que si un anticorps ne marche pas comme spécifié dans sa fiche technique (espèces et applications), nous offrons un remboursement ou un remplacement gratuit (https://www.abcam.com/abpromise).

Cet anticorps est un polyclonal de poulet. Nous avons des anticorps anti-poulet secondaires si cela vous intéresse :

Goat anti-Chicken IgY H&L (DyLight® 488) secondary antibody (ab96947)
Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 550) (ab96948)
Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 594) (ab96949)
Goat polyclonal Secondary Antibody to Chicken IgY - H&L (DyLight® 650) (ab96950)




J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Thank you for your inquiry.
I can confirm that ab4761 will detect beta Galactosidase from E.coli origin.
If beta Galactosidase is overexpressed (ectopically expressed) in human tissue than this antibody is suitable for this.
We tested this antibody with beta Galactosidase that was expressed in HeLa cells.
I hope this information is helpful and apologize for the confusion. Please feel free to contact me again if you have any further questions.

Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.
The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.
I would like to reassure you that ab4761 is tested and covered by our 6 month guarantee for use in samples that overexpress beta Galactosidase and IHC. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.
Reviewing this case, I would like to offer some suggestions to help optimise the results from ab4761.
1.) I recommend to use a permeabilization step since I assume that the beta Galactosidase will be overexpressed in the cytoplasm. 0.1% triton for 10 minutes only at RT.
2.) The BSA in the primary antibody solution can be omitted.
3.) I recommend to run a WB with the sample to confirm the expression of beta Galactosidase.
4.) It might be worth to try without antigen retrieval.
I would also appreciate if you can confirm some further details: 1.) Please give more details where the beta Galactosidase is overexpressed, what system was used and how the expression was tested.
2.) Is the secondary antibody and the rest of the detection reagents working?
I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

Thank you for your reply.

It is absolutely correct that you will get the full price of ab4761 as a discount off a future order.

If you would like to take part in this program, please let me know. I will be happy to issue a testing discount code for you that you will need to add to the additional notes sectionwhen submitting the Abreview and, of course, when you want to use the discount after submitting the Abreview.

I am looking forward to your reply.

Thank you very much for your interest in ab4761 and ab616.

I confirm that we cannot guarantee ab616 for IHC and we also do not recommend this antibody for this application.

To our knowledge, ab4761 has not been tested in IHC - Wholemount. Therefore, I can offer a discount off a future purchase if you buy ab4761 now, test it in IHC - Wholemountand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of ab4761 off a future order.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab4761 in IHC - Wholemount. I will then send a discount code. This code must be issued before purchasing ab4761 so please wait for my reply before ordering.

2. Purchase ab4761 either by phone, fax, or online (www.abcam.com).

3. Test it in IHC - Wholemount.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyproduct ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab4761 turns out to be unsuitable for IHC - Wholemount, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Thank you for your inquiry.
I am happy to confirm that ab4761 is tested and guaranteed for IHC-P.
The following publication even used this antibody with human/ viral transfected samples:
Hachiya A et al. Gene transfer in human skin with different pseudotyped HIV-based vectors. Gene Ther 14:648-56 (2007).
I attached the publication for more information on the protocol.
Because we carry over 75,000 products, it isn't feasible for us to keep small sample sizes of our products.
We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datasheet, or we will offer a replacement, credit, or refund within 6 months of purchase.
Otherwise, we like to encourage all of our customers to submit an Abreview via the online product datasheet. We always appreciate customer feedback, whether positive or negative, and we make all product information available to researchers. Plus, each Abreview earns Abpoints that can be used for discounts on future purchases or rewards such as Amazon.com gift certificates.
To find out more about our Abreview system, please see the following link:
https://www.abcam.com/abreviews
I hope this information is helpful and wish you good luck with your research.

Thank you for contacting us.

We have several antibodies against beta galactosidase, two of which have been validated for IHC staining of paraffin embedded sections. You may want to consider chicken polyclonal ab9361 or rabbit polyclonal ab4761. These have not been validated in human tissue specifically but should be compatible in detecting the Beta galactosidase protein.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.