Anti-beta Galactosidase抗体(ab134435)
Key features and details
- Chicken polyclonal to beta Galactosidase
- Suitable for: IHC-FoFr, ICC/IF
- Reacts with: Escherichia coli
- Isotype: IgY
概述
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产品名称
Anti-beta Galactosidase抗体
参阅全部 beta Galactosidase 一抗 -
描述
鸡多克隆抗体to beta Galactosidase -
宿主
Chicken -
经测试应用
适用于: IHC-FoFr, ICC/IFmore details -
种属反应性
与反应: Escherichia coli -
免疫原
Purified beta Galactosidase (lacZ) protein emulsified in Freund's adjuvants.
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阳性对照
- Tissues infected with a virus bearing the LacZ gene
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常规说明
Do not freeze these antibodies at –80°C unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half its binding activity is lost.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
存储溶液
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 49% PBS, 50% Glycerol -
Concentration information loading...
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纯度
IgY fraction -
纯化说明
IgY fractions were prepared from eggs yolks, and then affinity-purified antibodies were prepared using beta Galactosidase conjugated to an agarose matrix. The final product is a mixture of both affinity-purified antibodies (25 µg/ml) and purified IgY (10 mg/ml), and is filter-sterilized. -
克隆
多克隆 -
同种型
IgY -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab134435于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-FoFr |
1/2500 - 1/5000. Use 2% paraformaldehyde-fixed tissues infected with a virus bearing the LacZ gene.
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ICC/IF |
1/2500 - 1/5000. Use 2%
paraformaldehyde-fixed cells.
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说明 |
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IHC-FoFr
1/2500 - 1/5000. Use 2% paraformaldehyde-fixed tissues infected with a virus bearing the LacZ gene. |
ICC/IF
1/2500 - 1/5000. Use 2% paraformaldehyde-fixed cells. |
靶标
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相关性
Beta galactosidase is coded by a gene (lac z) in the lac operon of Escherichia coli. It is a metalloenzyme that splits lactose into glucose and galactose. It hydrolyzes terminal, non-reducing beta-D-galactose residues in beta-D-galactosides. Activation by cations seems to be substrate dependent. K+, Na+, NH4+, Rb+, Cs+ and Mn++ all activate enzyme activity based upon the substrate used. -
细胞定位
Cytoplasmic -
数据库链接
- Entrez Gene: 945006 Escherichia coli
- SwissProt: B7UJI9 Escherichia coli
- SwissProt: P00722 Escherichia coli
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别名
- Beta D galactosidase antibody
- Beta gal antibody
- Beta galactosidase antibody
see all
图片
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-beta Galactosidase antibody (ab134435)
Immunohistochemical analysis of tissue infected with a virus bearing the LacZ gene labelling beta Galactosidase with ab134435 at 1/5000 dilution with e18 mouse otic vesicle. The green stain is beta-galactosidase (ab134435); the red stain is Texas-red-phalloidin, staining filamentous actin; the blue staining is DAPI (4',6-diamidino-2-phenylindole), a DNA-binding dye.Secondary antibodies were fluorescein-labeled affinity-purified goat anti-chicken IgY (1/2000 dilution).
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (12)
ab134435 被引用在 12 文献中.
- Siddall NA et al. MiMIC analysis reveals an isoform specific role for Drosophila Musashi in follicle stem cell maintenance and escort cell function. Cell Death Discov 8:455 (2022). PubMed: 36371343
- Madhavan SM et al. Essential role of Wtip in mouse development and maintenance of the glomerular filtration barrier. Am J Physiol Renal Physiol 323:F272-F287 (2022). PubMed: 35862649
- Kim Y & Cho KO POU domain motif3 (Pdm3) induces wingless (wg) transcription and is essential for development of larval neuromuscular junctions in Drosophila. Sci Rep 10:517 (2020). PubMed: 31949274
- Jiang Z et al. MT1-MMP deficiency leads to defective ependymal cell maturation, impaired ciliogenesis, and hydrocephalus. JCI Insight 5:N/A (2020). PubMed: 32229724
- Spannl S et al. Glycolysis regulates Hedgehog signalling via the plasma membrane potential. EMBO J 39:e101767 (2020). PubMed: 33021744