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Anti-beta Dystroglycan (phospho Y892)抗体[M117] (ab29037)

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Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
  • Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)

Key features and details

  • Mouse monoclonal [M117] to beta Dystroglycan (phospho Y892)
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-beta Dystroglycan (phospho Y892)抗体[M117]
    参阅全部 beta Dystroglycan 一抗

  • 描述

    小鼠单克隆抗体[M117] to beta Dystroglycan (phospho Y892)

  • 宿主

    Mouse

  • 经测试应用

    适用于: WBmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Human beta Dystroglycan (phospho Y892) conjugated to keyhole limpet haemocyanin.

  • 阳性对照

    • HepG2 cells or HeLa cells treated with pervanadate.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab29037于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
说明
应用说明
ELISA: 1/2000.
WB: 1/1000. Membrane was incubated with diluted antibody in 5% non fat milk, PBS, 0.04% Tween 20 for 1 hour at room temperature. Predicted molecular weight: 97 kDa (Dystroglycan is expressed as a precursor protein that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

靶标

  • 相关性

    Dystroglycans are essential elements of the neuromuscular junction (NMJ). The gene for dystroglycan is expressed as a precursor protein that is post translationally cleaved into a 156 kDa extracellular peripheral membrane protein called alpha dystroglycan and a 43 kDa transmembrane protein, beta dystroglycan. The latter protein contains a PPxY motif that promotes binding to WW domain containing proteins, such as utrophin and dystrophin. Phosphorylation at tyrosine 892 within the PPxY motif may regulate c Src interactions with beta dystroglycan, as well as inhibit interactions with WW domain proteins. In skeletal muscle, beta dystroglycan is normally localized to the plasma membrane, however phosphorylation of Tyr892 leads to localization of beta dystroglycan to endosomal compartments along with c Src. Thus, phosphorylation at Tyr892 may have important roles in altering the localization of beta dystroglycan during NMJ formation.

  • 细胞定位

    Type I membrane protein.

  • 数据库链接

    • 别名

      • 156DAG antibody
      • A3a antibody
      • AGRNR antibody
      • Beta DG antibody
      • Beta dystroglycan antibody
      • DAG 1 antibody
      • DAG1 antibody
      • Dystroglycan antibody
      • Dystrophin associated glycoprotein 1 antibody
      see all

    图片

    • Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
      Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
      All lanes : Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)

      Lane 1 : Lysates from HepG2 cells (untreated)
      Lane 2 : Lysates from HepG2 cells treated with pervanadate (1 mM) for 30 min

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 97 kDa
      Observed band size: 43 kDa why is the actual band size different from the predicted?



      Dystroglycan is expressed as a precursor protein (97kDa) that is cleaved into alpha and beta dystroglycan. The beta dystroglycan fragment is about 43 kDa.
    • Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
      Western blot - Anti-beta Dystroglycan (phospho Y892) antibody [M117] (ab29037)
      Western blot analysis of HepG2 cells untreated (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 min (lanes 2 & 4). Blots were probed with anti-beta-dystroglycan (lanes 1 & 2) and anti-beta-dystroglycan (Tyr-892) (lanes 3 & 4).

    文献 (0)

    发表研究结果有使用 ab29037？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab29037 尚未被引用在任何文献中。

    客户评价及客户问答

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    Question

    I am currently investigating the antibodies against dystroglycan both alpha and beta and have seen that you have a large selection of different clones available. The antibodies with product codes mentioned above state they have not been referenced in papers yet... is this information up to date or are there still no references or evidence that these antibodies work. If this is the case it would be helpful if you could forward any information/evidence that you that these work. I would also be happy to test them if you were able to send them free of charge.

    Thank you for any assistance you can provide,

    Read More
    Answer

    Thank you for your inquiry.

    I can confirm that we do not know of any publication using the above mentioned antibodies. I would like to reassure you that we do guarantee our product for all species and applications stated on the respective datasheet.

    If you would like to use one of our products in a species or application that it has not been previously been tested in, you might be eligible for our testing discount program.

    https://www.abcam.com/collaborationdiscount

    For more information please see below:

    At Abcam, providing quality service is just as important as providing quality products. Our Abpromise® ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund.

    The Abcam Abpromise® guarantee:
    - 100% Scientific and Customer Support for any product you buy from Abcam or one of our authorized distributors.
    - We guarantee our products work in the tested species and applications stated on the datasheet.
    - We will replace or refund products not performing as stated on the datasheet if reported within 6 months of purchase.
    - We investigate any quality concerns raised by customers to ensure our catalog contains products that perform to the highest standards.

    Please note these conditions to our Abpromise®:
    - Protocol information must be provided in order for the claim to be validated.
    - Antibodies tested in recombinant samples only are not guaranteed for use on endogenous samples.
    - “Predicted to react” information on the datasheets is provided for reference only and these species are not guaranteed.
    - Fast Track antibodies are covered for ELISA against the immunizing peptide only.

    https://www.abcam.com/abpromise

    I hope this information is helpful, but please do not hesitate to contact us with further questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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