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Signal Transduction Protein Trafficking Chaperones

Anti-beta COP抗体(ab2899)

  • Datasheet
  • SDS
Reviews (15)Q&A (1)References (34)

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Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (ab2899)
  • Western blot - Anti-beta COP antibody (ab2899)
  • Western blot - Anti-beta COP antibody (ab2899)
  • Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (ab2899)
  • Western blot - Anti-beta COP antibody (ab2899)

Key features and details

  • Rabbit polyclonal to beta COP
  • Suitable for: IP, WB, ICC/IF
  • Reacts with: Mouse, Rat, Hamster, Cow, Human, Non human primates
  • Isotype: IgG

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概述

  • 产品名称

    Anti-beta COP抗体
    参阅全部 beta COP 一抗
  • 描述

    兔多克隆抗体to beta COP
  • 宿主

    Rabbit
  • 经测试应用

    适用于: IP, WB, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Rat, Hamster, Cow, Human, Non human primates
    预测可用于: Chicken, Pig
  • 免疫原

    Synthetic peptide corresponding to Rat beta COP aa 496-513.
    Sequence:

    EAGELKPEEEITVGPVQK

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • ICC/IF: L6 and NIH/3T3 cells; Rat brain homogenate. WB: HeLa, NIH/3T3, PC-3, C2C12, HCT116 and COLO 205 cell lysates; Mouse brain tissue lysate.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Ammonium Sulphate Precipitation
  • Primary antibody说明

    Coatomer proteins are involved in regulating transport between the endoplasmic reticulum (ER) and the Golgi complex and in intra-Golgi transport. There exist two coatomer-protein mechanisms (COP I and COP II) and although they have mechanistic parallels, they are molecularly distinct. The COP I coat is comprised of seven subunits (alpha-, beta-, beta'-, gamma-, delta-, epsilon-, and zeta-COP) in a complex called coatomer. Assembly of the coatomer (COP I) onto non-clathrin coated vesicles is regulated by ADP-ribosylation factor (ARF). Vesicle formation, budding, fusion, and disassembly is dependent on GDP-GTP exchange, COP I, and ARF. COP I has been shown to facilitate retrograde intracellular transport from the ER to the Golgi complex. By contrast, COP II facilitates anterograde transport between these subcellular organelles. COP II has been shown to be independently and selectively recruited to the ER relative to COP I subunits.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Protein Trafficking
    • Chaperones
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • BALB/3T3 whole cell lysate (ab7901)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2899于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IP (1)
Use at an assay dependent concentration. PubMed: 18556652
WB (8)
1/1000. Detects a band of approximately 110 kDa.
ICC/IF (6)
1/2000.

Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion.

说明
IP
Use at an assay dependent concentration. PubMed: 18556652
WB
1/1000. Detects a band of approximately 110 kDa.
ICC/IF
1/2000.

Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion.

靶标

  • 功能

    The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Plays a functional role in facilitating the transport of kappa-type opioid receptor mRNAs into axons and enhances translation of these proteins. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte surface triglyceride lipase (PNPLA2) with the lipid droplet to mediate lipolysis (By similarity). Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1. Promotes degradation of Nef cellular targets CD4 and MHC class I antigens by facilitating their trafficking to degradative compartments.
  • 序列相似性

    Contains 6 HEAT repeats.
  • 翻译后修饰

    Proteolytically cleaved between Ser-528 and Ser-529 by CAPN8.
  • 细胞定位

    Cytoplasm. Golgi apparatus membrane. Cytoplasmic vesicle > COPI-coated vesicle membrane. Cell membrane. Endoplasmic reticulum-Golgi intermediate compartment. The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it. Proteolytic cleavage by CAPN8 triggers translocation from Golgi to cytoplasm (By similarity). Found in perinuclear vesicular-tubular clusters (VTCs) and in the Golgi region where associated with vesicles, buds and rims of the Golgi stack (By similarity). Occasionally present at the trans-side of Golgi, but mainly present at the cis-Golgi side in transitional areas (TA), on so-called peripheral elements (PE) consisting of tubules and vesicles located between the cup-shaped transitional elements (TE) of the rough endoplasmic reticulum (RER) and the cis-most Golgi cisternae (By similarity). Present in cytoplasm, not associated with visible coats or membranes, with a minor fraction present on small clusters of tubules and vesicles (By similarity). Some association with high-density and low-density microsomes and mitochondria/nuclei fraction (By similarity). Very little found in plasma membrane fraction.
  • Target information above from: UniProt accession P53618 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 423063 Chicken
    • Entrez Gene: 535533 Cow
    • Entrez Gene: 1315 Human
    • Entrez Gene: 70349 Mouse
    • Entrez Gene: 100337660 Pig
    • Entrez Gene: 114023 Rat
    • Omim: 600959 Human
    • SwissProt: Q5ZIA5 Chicken
    • SwissProt: A0JN39 Cow
    • SwissProt: P53618 Human
    • SwissProt: Q9JIF7 Mouse
    • SwissProt: D2SW95 Pig
    • SwissProt: P23514 Rat
    • Unigene: 339278 Human
    • Unigene: 277024 Mouse
    • Unigene: 4327 Rat
    see all
  • 别名

    • Beta-coat protein antibody
    • Beta-COP antibody
    • betacop antibody
    • Coatomer beta subunit antibody
    • Coatomer protein complex subunit beta 1 antibody
    • Coatomer protein complex subunit beta antibody
    • Coatomer subunit beta antibody
    • COPB antibody
    • COPB_HUMAN antibody
    • Copb1 antibody
    • DKFZp761K102 antibody
    • FLJ10341 antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (ab2899)
    Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (ab2899)

    Immunolocalization of beta COP in NIH-3T3 cells using ab2899

  • Western blot - Anti-beta COP antibody (ab2899)
    Western blot - Anti-beta COP antibody (ab2899)
    All lanes : Anti-beta COP antibody (ab2899) at 2 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 3 : PC-3 (Human prostate adenocarcinoma cell line) whole cell lysate
    Lane 4 : C2C12 (Mouse myoblast cell line) whole cell lysate
    Lane 5 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
    Lane 6 : HCT116 (Human colorectal carcinoma cell line) whole cell lysate
    Lane 7 : COLO 205 (Human colon adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Western blot analysis was performed on membrane enriched extracts (30 µg) of cells. The blots were probed with ab2899 (2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate. A ~107 kDa band corresponding beta COP was observed across tissue and cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE®4-12 % Bis-Tris gel, XCell SureLock™ Electrophoresis System and Novex® Sharp Pre-Stained Protein Standard. Resolved proteins were then transferred onto a nitrocellulose membrane by Pierce™ Power Blotter System. The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate.

  • Western blot - Anti-beta COP antibody (ab2899)
    Western blot - Anti-beta COP antibody (ab2899)
    All lanes : Anti-beta COP antibody (ab2899) at 2 µg/ml

    Lane 1 : Untransfected HeLa cell lysate
    Lane 2 : HeLa (transfected with non-targeting scrambled siRNA) cell lysate
    Lane 3 : HeLa (transfected with beta COP specific siRNAs) cell lysate


    Knockdown of beta COP was achieved by transfecting HeLa with beta COP specific siRNAs. Western blot analysis was performed using Membrane Enriched extracts from the beta COP knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with ab2899 at 2ug/ml and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (1:4000 dilution). 

  • Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (ab2899)
    Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (ab2899)

    Immunofluorescent analysis of beta COP was performed using 70% confluent log phase L6 (Rat skeletal muscle cell line) cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab2899 at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

  • Western blot - Anti-beta COP antibody (ab2899)
    Western blot - Anti-beta COP antibody (ab2899)
    Western blot of beta-COP on rat brain homogenate using ab2899. Western blot of beta-COP on rat brain homogenate using ab2899.

实验方案

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (34)

发表研究结果有使用 ab2899?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab2899 被引用在 34 文献中.

  • Morelli E  et al. Activity of the SNARE Protein SNAP29 at the Endoplasmic Reticulum and Golgi Apparatus. Front Cell Dev Biol 9:637565 (2021). PubMed: 33718375
  • Macken WL  et al. Biallelic variants in COPB1 cause a novel, severe intellectual disability syndrome with cataracts and variable microcephaly. Genome Med 13:34 (2021). PubMed: 33632302
  • Guo S  et al. Trio cooperates with Myh9 to regulate neural crest-derived craniofacial development. Theranostics 11:4316-4334 (2021). PubMed: 33754063
  • Albert MC  et al. CHIP ubiquitylates NOXA and induces its lysosomal degradation in response to DNA damage. Cell Death Dis 11:740 (2020). PubMed: 32913203
  • Sakaguchi N  et al. Role of Gate-16 and Gabarap in Prevention of Caspase-11-Dependent Excess Inflammation and Lethal Endotoxic Shock. Front Immunol 11:561948 (2020). PubMed: 33042141
View all Publications for this product

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Western blot abreview for Anti-beta COP antibody

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Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293T)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
HEK293T
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Jerome Cattin

Verified customer

提交于 May 20 2020

Western blot abreview for Anti-beta COP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (retina)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
20 µg
Specification
retina
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Feb 12 2020

Immunocytochemistry/ Immunofluorescence abreview for Anti-beta COP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice Hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice Hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

提交于 Apr 02 2019

Western blot abreview for Anti-beta COP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mice Hepatocytes)
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Loading amount
35 µg
Specification
Mice Hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

提交于 Feb 19 2019

Western blot abreview for Anti-beta COP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse Podocytes)
Gel Running Conditions
Reduced Denaturing
Loading amount
50 µg
Specification
Mouse Podocytes
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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提交于 Mar 27 2018

Western blot abreview for Anti-beta COP antibody

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Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (DU145 - prostate cancer cells)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
26 µg
Specification
DU145 - prostate cancer cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

提交于 Sep 28 2017

Western blot abreview for Anti-beta COP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions
Reduced Denaturing (4-15% SDS PAGE)
Loading amount
15 µg
Specification
Rat hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

提交于 Apr 07 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-beta COP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (RWPE - prostate cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
RWPE - prostate cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

提交于 Mar 31 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-beta COP antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

DR. Armen Petrosyan

Verified customer

提交于 Mar 28 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-beta COP antibody

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Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - Triton X-100
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 23°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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Verified customer

提交于 Oct 02 2012

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