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AB2899

Anti-beta COP 抗体

Anti-beta COP antibody

5

(15 Reviews)

|

(52 Publications)

Rabbit Polyclonal beta COP antibody. Suitable for ICC/IF, IP, WB and reacts with Mouse, Rat, Primates, Hamster, Human, Cow samples. Cited in 52 publications. Immunogen corresponding to Synthetic Peptide within Rat Copb1 aa 450-550.

查看别名

COPB, MSTP026, COPB1, Coatomer subunit beta, Beta-coat protein, Beta-COP

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (AB2899)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (AB2899)

Immunolocalization of beta COP in NIH-3T3 cells using ab2899

Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (AB2899)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-beta COP antibody (AB2899)

Immunofluorescent analysis of beta COP was performed using 70% confluent log phase L6 (Rat skeletal muscle cell line) cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab2899 at 2 μg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate a dilution of 1 : 2000 for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with SlowFade® Gold Antifade Mountant with DAPI. F-actin (Panel c : red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin. Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Rat, Human, Primates, Cow, Hamster

应用

WB, ICC/IF, IP

applications

免疫原

Synthetic Peptide within Rat Copb1 aa 450-550. The exact immunogen used to generate this antibody is proprietary information.

P23514

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替代产品

Primary Antibodies

AB264352

Anti-beta COP antibody

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搭配使用产品

Primary Antibodies

AB21679

Anti-Clathrin heavy chain antibody

Western blot - Anti-Clathrin heavy chain antibody (AB21679)

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Primary Antibodies

AB96725

Anti-Coatomer subunit delta/COPD antibody

Immunocytochemistry/ Immunofluorescence - Anti-Coatomer subunit delta/COPD antibody (AB96725)

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Primary Antibodies

AB52649

Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

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Immunoprecipitation - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (AB52649)

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Primary Antibodies

AB192919

Anti-alpha COP I/COPA antibody - C-terminal

Western blot - Anti-alpha COP I/COPA antibody - C-terminal (AB192919)

  • 0
  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "<p>Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion.</p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "<p>Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion.</p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Chicken": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Hamster": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Primates": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "1/2000", "ICCIF-species-notes": "<p>Golgi localization of beta-COP is sensitive to treatment with brefeldin A or ATP depletion.</p>", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Precipitation Ammonium Sulphate
存储溶液
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Beta COP also known as coatomer subunit beta and sometimes referred to as beta COPI is an important component of the coatomer protein complex involved in vesicle formation and trafficking. This protein has a molecular mass of approximately 110 kDa. Beta COP is expressed widely across various tissues and is essential in maintaining cellular transport systems. It resides mainly within the cytosolic coat of COPI vesicles that originate from Golgi membranes helping in directing the flow of enzymes that are vital for cellular metabolism.
Biological function summary

Coatomer subunits like beta COP play an integral role in forming the COPI complex which functions as a coat on Golgi-derived transport vesicles. Beta COP acts to mediate retrograde transport from the Golgi apparatus back to the endoplasmic reticulum. By helping in vesicle budding and fusion processes it ensures correct protein sorting and localization. This mechanism is critical for cellular homeostasis and proper cellular functions.

Pathways

The COPI-mediated transport involving beta COP plays a significant role in the Golgi-to-ER retrograde transport pathway. This pathway is vital for maintaining the balance of lipids and proteins within cells. Key proteins interacting with this pathway include other coatomer subunits such as delta and gamma COP. Through these interactions beta COP is indispensable in regulating vesicle trafficking and membrane dynamics affecting overall cellular communication.

Beta COP's malfunction can lead to disruptions in vesicle transport potentially contributing to disorders like genetic syndromes or neurodegenerative diseases. Research suggests a connection between aberrant COPI transport and pathology such as Parkinson's disease where beta COP might interact with protein alpha-synuclein. Additionally alterations in beta COP's function link to Alzheimer’s disease where its dysfunction could impact amyloid precursor protein processing. Understanding these connections offers pathways to exploring therapeutic interventions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors. Plays a functional role in facilitating the transport of kappa-type opioid receptor mRNAs into axons and enhances translation of these proteins. Required for limiting lipid storage in lipid droplets. Involved in lipid homeostasis by regulating the presence of perilipin family members PLIN2 and PLIN3 at the lipid droplet surface and promoting the association of adipocyte surface triglyceride lipase (PNPLA2) with the lipid droplet to mediate lipolysis (By similarity). Involved in the Golgi disassembly and reassembly processes during cell cycle. Involved in autophagy by playing a role in early endosome function. Plays a role in organellar compartmentalization of secretory compartments including endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC), Golgi, trans-Golgi network (TGN) and recycling endosomes, and in biosynthetic transport of CAV1. Promotes degradation of Nef cellular targets CD4 and MHC class I antigens by facilitating their trafficking to degradative compartments.
See full target information COPB1
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文献 (52)

Recent publications for all applications. Explore the full list and refine your search

Journal of virology 99:e0096225 PubMed40838750

2025

Coatomer protein complex I is required for efficient secretion of dengue virus non-structural protein 1.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen M Johnson,Siena M Centofanti,Gustavo Bracho,Michael R Beard,Jillian M Carr,Nicholas S Eyre

Nature communications 16:3715 PubMed40253417

2025

A hollow TFG condensate spatially compartmentalizes the early secretory pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Savannah M Bogus,William R Wegeng,Miguel Ruiz,Sindy R Chavez,Samantha N Cheung,Khalid S M Noori,Ingrid R Niesman,Andreas M Ernst

Cell discovery 11:18 PubMed40038246

2025

TMEM41B is an endoplasmic reticulum Ca release channel maintaining naive T cell quiescence and responsiveness.

Applications

Unspecified application

Species

Unspecified reactive species

Yuying Ma,Yi Wang,Xiaocui Zhao,Gang Jin,Jing Xu,Zhuoyang Li,Na Yin,Zhaobing Gao,Bingqing Xia,Min Peng

mBio 16:e0333124 PubMed39611845

2024

Coatomer complex I is required for the transport of SARS-CoV-2 progeny virions from the endoplasmic reticulum-Golgi intermediate compartment.

Applications

Unspecified application

Species

Unspecified reactive species

Ai Hirabayashi,Yukiko Muramoto,Toru Takenaga,Yugo Tsunoda,Mayumi Wakazaki,Mayuko Sato,Yoko Fujita-Fujiharu,Norimichi Nomura,Koji Yamauchi,Chiho Onishi,Masahiro Nakano,Kiminori Toyooka,Takeshi Noda

Cell communication and signaling : CCS 22:140 PubMed38378560

2024

Crosstalk between KDEL receptor and EGF receptor mediates cell proliferation and migration via STAT3 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Jia,Lianhui Zhu,Xihua Yue,Shuocheng Tang,Shuaiyang Jing,Chuanting Tan,Yulei Du,Jingkai Gao,Intaek Lee,Yi Qian

International journal of molecular sciences 25: PubMed38203512

2024

COPI Vesicle Disruption Inhibits Mineralization via mTORC1-Mediated Autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaming Nie,Shaoyang Ma,Yuchen Zhang,Shuchen Yu,Jiajia Yang,Ang Li,Dandan Pei

Nature communications 14:8358 PubMed38102143

2023

A single C-terminal residue controls SARS-CoV-2 spike trafficking and incorporation into VLPs.

Applications

Unspecified application

Species

Unspecified reactive species

Debajit Dey,Enya Qing,Yanan He,Yihong Chen,Benjamin Jennings,Whitaker Cohn,Suruchi Singh,Lokesh Gakhar,Nicholas J Schnicker,Brian G Pierce,Julian P Whitelegge,Balraj Doray,John Orban,Tom Gallagher,S Saif Hasan

Nature communications 14:6770 PubMed37914730

2023

ARF1 prevents aberrant type I interferon induction by regulating STING activation and recycling.

Applications

Unspecified application

Species

Unspecified reactive species

Maximilian Hirschenberger,Alice Lepelley,Ulrich Rupp,Susanne Klute,Victoria Hunszinger,Lennart Koepke,Veronika Merold,Blaise Didry-Barca,Fanny Wondany,Tim Bergner,Tatiana Moreau,Mathieu P Rodero,Reinhild Rösler,Sebastian Wiese,Stefano Volpi,Marco Gattorno,Riccardo Papa,Sally-Ann Lynch,Marte G Haug,Gunnar Houge,Kristen M Wigby,Jessica Sprague,Jerica Lenberg,Clarissa Read,Paul Walther,Jens Michaelis,Frank Kirchhoff,Carina C de Oliveira Mann,Yanick J Crow,Konstantin M J Sparrer

Nature communications 14:2683 PubMed37160917

2023

Zinc homeostasis governed by Golgi-resident ZnT family members regulates ERp44-mediated proteostasis at the ER-Golgi interface.

Applications

Unspecified application

Species

Unspecified reactive species

Yuta Amagai,Momo Yamada,Toshiyuki Kowada,Tomomi Watanabe,Yuyin Du,Rong Liu,Satoshi Naramoto,Satoshi Watanabe,Junko Kyozuka,Tiziana Anelli,Tiziana Tempio,Roberto Sitia,Shin Mizukami,Kenji Inaba

Nature communications 14:948 PubMed36804936

2023

Identification of global inhibitors of cellular glycosylation.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Madriz Sørensen,Christian Büll,Thomas D Madsen,Erandi Lira-Navarrete,Thomas Mandel Clausen,Alex E Clark,Aaron F Garretson,Richard Karlsson,Johan F A Pijnenborg,Xin Yin,Rebecca L Miller,Sumit K Chanda,Thomas J Boltje,Katrine T Schjoldager,Sergey Y Vakhrushev,Adnan Halim,Jeffrey D Esko,Aaron F Carlin,Ramon Hurtado-Guerrero,Roberto Weigert,Henrik Clausen,Yoshiki Narimatsu
View all publications
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