Anti-beta Catenin (phospho S33)抗体(ab53050)
Key features and details
- Rabbit polyclonal to beta Catenin (phospho S33)
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-beta Catenin (phospho S33)抗体
参阅全部 beta Catenin 一抗 -
描述
兔多克隆抗体to beta Catenin (phospho S33) -
宿主
Rabbit -
经测试应用
适用于: IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide corresponding to Human beta Catenin (phospho S33).
Sequence:Sp-G-I-H-S
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg+2 and Ca+2 -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab53050 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab53050于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration.
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| 说明 |
|---|
|
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Key dowstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.
Involved in the regulation of cell adhesion. The majority of beta-catenin is localized to the cell membrane and is part of E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton. -
组织特异性
Expressed in several hair follicle cell types: basal and peripheral matrix cells, and cells of the outer and inner root sheaths. Expressed in colon. -
疾病相关
Defects in CTNNB1 are associated with colorectal cancer (CRC) [MIM:114500].
Note=Activating mutations in CTNNB1 have oncogenic activity resulting in tumor development. Somatic mutations are found in various tumor types, including colon cancers, ovarian and prostate carcinomas, hepatoblastoma (HB), hepatocellular carcinoma (HCC). HBs are malignant embryonal tumors mainly affecting young children in the first three years of life.
Defects in CTNNB1 are a cause of pilomatrixoma (PTR) [MIM:132600]; a common benign skin tumor.
Defects in CTNNB1 are a cause of medulloblastoma (MDB) [MIM:155255]. MDB is a malignant, invasive embryonal tumor of the cerebellum with a preferential manifestation in children.
Defects in CTNNB1 are a cause of susceptibility to ovarian cancer (OC) [MIM:167000]. Ovarian cancer common malignancy originating from ovarian tissue. Although many histologic types of ovarian neoplasms have been described, epithelial ovarian carcinoma is the most common form. Ovarian cancers are often asymptomatic and the recognized signs and symptoms, even of late-stage disease, are vague. Consequently, most patients are diagnosed with advanced disease.
Note=A chromosomal aberration involving CTNNB1 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1. -
序列相似性
Belongs to the beta-catenin family.
Contains 12 ARM repeats. -
翻译后修饰
Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33.
EGF stimulates tyrosine phosphorylation. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding.
Ubiquitinated by the SCF(BTRC) E3 ligase complex when phosphorylated by GSK3B, leading to its degradation. Ubiquitinated by a E3 ubiquitin ligase complex containing UBE2D1, SIAH1, CACYBP/SIP, SKP1, APC and TBL1X, leading to its subsequent proteasomal degradation. -
细胞定位
Cytoplasm. Nucleus. Cytoplasm > cytoskeleton. Cell junction > adherens junction. Cell junction. Cell membrane. Cytoplasmic when it is unstabilized (high level of phosphorylation) or bound to CDH1. Translocates to the nucleus when it is stabilized (low level of phosphorylation). Interaction with GLIS2 and MUC1 promotes nuclear translocation. Interaction with EMD inhibits nuclear localization. - Information by UniProt
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数据库链接
- Entrez Gene: 1499 Human
- Omim: 116806 Human
- SwissProt: P35222 Human
- Unigene: 476018 Human
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别名
- b-catenin antibody
- Beta catenin antibody
- Beta-catenin antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Catenin (phospho S33 + S37) antibody (ab53050)ab53050 at 1/50 dilution staining beta Catenin in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue (in the absence or presence of the immunising peptide).
数据表及文件
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SDS download
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Datasheet download
文献 (8)
ab53050 被引用在 8 文献中.
- Wang L et al. MiR-543 Inhibits the Migration and Epithelial-To-Mesenchymal Transition of TGF-ß-Treated Endometrial Stromal Cells via the MAPK and Wnt/ß-Catenin Signaling Pathways. Pathol Oncol Res 27:1609761 (2021). PubMed: 34257616
- Ma C et al. Hippo/Mst1 overexpression induces mitochondrial death in head and neck squamous cell carcinoma via activating ß-catenin/Drp1 pathway. Cell Stress Chaperones 24:807-816 (2019). PubMed: 31127452
- Zhang L et al. Anti-tumor effect of LATS2 on liver cancer death: Role of DRP1-mediated mitochondrial division and the Wnt/ß-catenin pathway. Biomed Pharmacother 114:108825 (2019). PubMed: 30981110
- Shi W et al. NR4A1 silencing protects against renal ischemia-reperfusion injury through activation of the ß-catenin signaling pathway in old mice. Exp Mol Pathol 111:104303 (2019). PubMed: 31465766
- Zhao H et al. Sirt3 inhibits cerebral ischemia-reperfusion injury through normalizing Wnt/ß-catenin pathway and blocking mitochondrial fission. Cell Stress Chaperones 23:1079-1092 (2018). PubMed: 29862442
- Xue W et al. CRISPR-mediated direct mutation of cancer genes in the mouse liver. Nature N/A:N/A (2014). Mouse . PubMed: 25119044
- Kan A & Tabin CJ c-Jun is required for the specification of joint cell fates. Genes Dev 27:514-24 (2013). Mouse . PubMed: 23475960
- Morrogh M et al. Cadherin-catenin complex dissociation in lobular neoplasia of the breast. Breast Cancer Res Treat : (2011). IHC-P ; Human . PubMed: 22080244