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AB281143

Anti-beta Amyloid precursor protein 抗体 [EPR22445-191] - BSA and Azide free (Capture)

Anti-beta Amyloid precursor protein antibody [EPR22445-191] - BSA and Azide free (Capture)

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Anti-beta Amyloid precursor protein antibody [EPR22445-191] - BSA and Azide free (Capture) (ab281143) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation. Suitable for ELISA in Human, Mouse, Rat.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

查看别名

A4, AD1, App, Amyloid-beta precursor protein, ABPP, Alzheimer disease amyloid A4 protein homolog, Alzheimer disease amyloid protein, Amyloid precursor protein, Amyloid-beta (A4) precursor protein, Amyloid-beta A4 protein, Amyloidogenic glycoprotein, APP, AG

2 Images
Sandwich ELISA - Anti-beta Amyloid precursor protein antibody [EPR22445-191] - BSA and Azide free (Capture) (AB281143)
  • sELISA

Lab

Sandwich ELISA - Anti-beta Amyloid precursor protein antibody [EPR22445-191] - BSA and Azide free (Capture) (AB281143)

Sandwich ELISA of ab253583 with the capture antibody ab281143 dilution at 2 µg/mL and detector antibody ab280993 dilution at 0.5 µg/mL.

Sandwich ELISA - Anti-beta Amyloid precursor protein antibody [EPR22445-191] - BSA and Azide free (Capture) (AB281143)
  • sELISA

Lab

Sandwich ELISA - Anti-beta Amyloid precursor protein antibody [EPR22445-191] - BSA and Azide free (Capture) (AB281143)

Sandwich ELISA of ab253686 with the capture antibody ab281143 dilution at 2 µg/mL and detector antibody ab281096 dilution at 0.5 µg/mL.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR22445-191

亚型

IgG

不含载体蛋白

Yes

反应种属

Mouse, Rat, Human

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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搭配使用产品

Assay Kits

AB150663

Congo Red Stain Kit (Amyloid Stain)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Congo Red Stain Kit (Amyloid Stain) (AB150663)

  • 0
  • 0
Biochemicals

AB120301

beta-Amyloid Peptide (1-42) (human)

Chemical Structure - beta-Amyloid Peptide (1-42) (human) (AB120301)

  • 5
  • 1
Sample Prep & Detection Kits

AB270705

5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20

Schematic Diagram - 5’ Feature Barcode Antibody Conjugation Kit - Lightning-Link® - Oligos 11-20 (AB270705)

  • 0
  • 0
Sample Prep & Detection Kits

AB270228

Europium-Streptavidin

Functional Studies - Europium-Streptavidin (AB270228)

  • 5
  • 1
Primary Antibodies

AB242692

Anti-GFAP antibody [EPR19996-132] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-GFAP antibody [EPR19996-132] - BSA and Azide free (Capture) (AB242692)

  • 0
  • 0
Sample Prep & Detection Kits

AB201795

Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link®

ELISA - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (AB201795)

  • 0
  • 0
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Rat": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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产品详情

ab281143 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Amyloid-beta 42 protein. The recommended pair for sandwich ELISA is:
Capture: ab281143, Human Amyloid-beta 42 protein Capture Antibody (unconjugated)
Detector: ab280993, Human Amyloid-beta 42 protein Detector Antibody (unconjugated)
The reference range value is 7.81-500 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Amyloid Precursor Protein (APP) also known as amyloid protein is a transmembrane protein that is approximately 695 to 770 amino acids in length depending on the isoform. The molecular mass of APP can vary but typically falls around 100 to 140 kDa. It is heavily expressed in the central nervous system particularly in neurons but also in other tissues like muscle and kidney. The APP undergoes proteolytic processing which leads to the generation of various fragments including beta-amyloid peptides.
Biological function summary

The processing of APP plays a fundamental role in neuronal growth survival and repair. APP is cleaved into fragments that can regulate synaptic function and plasticity. It does not operate as a part of a complex but interacts with various cellular components. The protein participates in signaling pathways influencing cellular adhesion motility and neurite outgrowth. APP’s numerous interaction partners facilitate its involvement in different cellular processes highlighting its critical role in normal cell function.

Pathways

The APP is a central component in the amyloidogenic pathway where its cleavage by beta-secretase and gamma-secretase yields beta-amyloid. This pathway is one of two primary metabolic routes for APP—alternative enzymatic processing through the non-amyloidogenic pathway precludes beta-amyloid formation releasing peptides that do not aggregate. Enzymes like BACE1 (beta-secretase 1) and presenilin are important in the amyloidogenic pathway directly resulting in the production of the neurotoxic amyloid beta-peptide.

APP is intensely linked to Alzheimer's disease and cerebral amyloid angiopathy. Accumulation of beta-amyloid peptides formed from APP cleavage is a hallmark of Alzheimer's disease leading to plaque formation in the brain. This aggregation impacts neuronal function and is associated with neurodegenerative processes. Interactions with proteins like tau are significant as tau also plays an essential role in Alzheimer's disease pathology. Misprocessing of APP and the resulting beta-amyloid aggregates are also contributors to cerebral amyloid angiopathy where deposits within cerebrovascular walls compromise vascular integrity.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Functions as a cell surface receptor and performs physiological functions on the surface of neurons relevant to neurite growth, neuronal adhesion and axonogenesis. Interaction between APP molecules on neighboring cells promotes synaptogenesis. Involved in cell mobility and transcription regulation through protein-protein interactions. Can promote transcription activation through binding to APBB1-KAT5 and inhibit Notch signaling through interaction with Numb. Couples to apoptosis-inducing pathways such as those mediated by G(o) and JIP. Inhibits G(o)-alpha ATPase activity (By similarity). Acts as a kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1 (By similarity). By acting as a kinesin I membrane receptor, plays a role in axonal anterograde transport of cargo towards synapses in axons (By similarity). May be involved in copper homeostasis/oxidative stress through copper ion reduction. Can regulate neurite outgrowth through binding to components of the extracellular matrix such as heparin and collagen I and IV (By similarity). The splice isoforms that contain the BPTI domain possess protease inhibitor activity. Induces a AGER-dependent pathway that involves activation of p38 MAPK, resulting in internalization of amyloid-beta peptide and leading to mitochondrial dysfunction in cultured cortical neurons (By similarity). Provides Cu(2+) ions for GPC1 which are required for release of nitric oxide (NO) and subsequent degradation of the heparan sulfate chains on GPC1.. Amyloid-beta peptides are lipophilic metal chelators with metal-reducing activity. Binds transient metals such as copper, zinc and iron. Rat and mouse amyloid-beta peptides bind only weakly transient metals and have little reducing activity due to substitutions of transient metal chelating residues. Amyloid-beta protein 42 may activate mononuclear phagocytes in the brain and elicit inflammatory responses. Promotes both tau aggregation and TPK II-mediated phosphorylation. Also binds GPC1 in lipid rafts (By similarity).. The gamma-CTF peptides as well as the caspase-cleaved peptides, including C31, are potent enhancers of neuronal apoptosis.
See full target information App
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