重组Anti-beta Actin抗体[BLR057F] - BSA free (ab272085)
![Western blot - Anti-beta Actin antibody [BLR057F] - BSA free (ab272085)](https://www.abcam.cn/ps/products/272/ab272085/Images/ab272085-467892-272085-wb.JPG "Western blot - Anti-beta Actin antibody [BLR057F] - BSA free (ab272085)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR057F] to beta Actin - BSA free
- Suitable for: IHC-P, ICC, WB
- Reacts with: Mouse, Human
Related conjugates and formulations
概述
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产品名称
Anti-beta Actin抗体[BLR057F] - BSA free
参阅全部 beta Actin 一抗 -
描述
兔单克隆抗体[BLR057F] to beta Actin - BSA free -
宿主
Rabbit -
经测试应用
适用于: IHC-P, ICC, WBmore details -
种属反应性
与反应: Mouse, Human -
免疫原
Synthetic peptide within Human beta Actin aa 1-50. The exact sequence is proprietary. (NP_001092.1).
Database link: P60709 -
阳性对照
- IHC-P: Human ovartian carcinoma tissue. ICC: HeLa cells. WB: U2-OS, A549, K562, HeLa, HEK-293T, Jurkat, TCMK-1 and NIH/3T3 cell lysates.
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常规说明
ab272085 is the BSA-free version of ab252556.
This product is sold under License from Bethyl Laboratories, Inc.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 8.20
Preservative: 0.09% Sodium azide
Constituent: 99% Borate buffered saline -
Concentration information loading... -
纯化说明
Recombinant antibody was purified from cell culture supernatant. -
克隆
单克隆 -
克隆编号
BLR057F -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab272085于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| ICC |
Use at an assay dependent concentration.
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| WB |
1/1000. Predicted molecular weight: 41 kDa.
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| 说明 |
|---|
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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ICC
Use at an assay dependent concentration. |
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WB
1/1000. Predicted molecular weight: 41 kDa. |
靶标
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功能
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. -
疾病相关
Defects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss. -
序列相似性
Belongs to the actin family. -
翻译后修饰
ISGylated. -
细胞定位
Cytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. - Information by UniProt
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数据库链接
- Entrez Gene: 60 Human
- Entrez Gene: 11461 Mouse
- Omim: 102630 Human
- SwissProt: P60709 Human
- SwissProt: P60710 Mouse
- Unigene: 520640 Human
- Unigene: 708120 Human
- Unigene: 727576 Human
see all -
别名
- A26C1A antibody
- A26C1B antibody
- ACTB antibody
see all
图片
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Western blot - Anti-beta Actin antibody [BLR057F] - BSA free (ab272085)All lanes : Anti-beta Actin antibody [BLR057F] (ab252556) at 1/1000 dilution
Lane 1 : U2-OS (human bone osteosarcoma epithelial cell line) whole cell lysate
Lane 2 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 7 : TCMK-1 (mouse kidney epithelial cell line) whole cell lysate
Lane 8 : NIH/3T3 (mouse embyro fibroblast cell line) whole cell lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG
Developed using the ECL technique.
Predicted band size: 41 kDa
Exposure time: 1 secondThis data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252556).
Lysates prepared using NETN lysis buffer.
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![Immunocytochemistry - Anti-beta Actin antibody [BLR057F] - BSA free (ab272085)](https://www.abcam.cn/ps/products/272/ab272085/Images/ab272085-2-ab252556343365antibetaActinantibodyBLR057FimmunocytochemistryHeLahuman.jpg)
Immunocytochemistry - Anti-beta Actin antibody [BLR057F] - BSA free (ab272085)FFPE HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for beta Actin using ab252556 at 1/200 dilution in ICC/IF, followed by HRP conjugated Goat Anti-Rabbit IgG. Substrate: DAB.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252556).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [BLR057F] - BSA free (ab272085)](https://www.abcam.cn/ps/products/272/ab272085/Images/ab272085-1-ab252556343366antibetaActinantibodyBLR057Fimmunohistochemistryovariancarcinomahuman.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [BLR057F] - BSA free (ab272085)Formalin-fixed, paraffin-embedded human ovarian carcinoma tissue stained for beta Actin using ab252556 at 1/400 dilution in immunohistochemical analysis, followed by HRP conjugated. DAB staining.
This data was developed using the same antibody clone in a different buffer formulation containing Borate buffered saline, BSA, glycerol and sodium azide (ab252556).
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![Anti-beta Actin antibody [BLR057F] - BSA free (ab272085)](https://www.abcam.cn/ps/products/272/ab272085/Images/ab272085-3-benefits-of-recombinant-antibodies.png)
Anti-beta Actin antibody [BLR057F] - BSA free (ab272085)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab272085 尚未被引用在任何文献中。