AB300484

重组Anti-beta 3 Adrenergic Receptor抗体[EPR25440-151] (BSA and Azide free)

Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151] (BSA and Azide free)

RabMAb
Recombinant
了解详情

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal beta 3 Adrenergic Receptor antibody. Carrier free. Suitable for WB and reacts with Human samples.

查看别名

ADRB3R, B3AR, ADRB3, Beta-3 adrenergic receptor, Beta-3 adrenoreceptor, Beta-3 adrenoceptor

不同偶联物与剂型 (1)

Unconjugated

Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR25440-151

亚型

IgG

不含载体蛋白

Yes

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

style

left-column

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The beta 3 adrenergic receptor also known as beta-3 adrenoceptor or B3 adrenergic receptor is a member of the adrenergic receptor family that activates the GS protein upon binding ligands like catecholamines. This receptor approximately 408 amino acids in size has a predominant mass of around 45 kDa. It is mainly expressed in adipose tissue but also found in the gallbladder urinary bladder and the gastrointestinal tract. These receptors primarily mediate lipolysis and thermogenesis in brown adipose tissue through interaction with norepinephrine and epinephrine.

Biological function summary

The beta 3 adrenergic receptor plays an important role in energy expenditure and metabolic processes. It does not typically form part of a complex but interacts with GS proteins leading to the activation of adenylyl cyclase and consequent increase in cAMP levels. This receptor is instrumental in the regulation of lipid metabolism which aids in maintaining balance in energy store.

Pathways

The beta 3 adrenergic receptor is involved in the adrenergic signaling pathways regulating energy expenditure and thermogenesis. These pathways often work together with other adrenergic receptors such as beta 1 and beta 2 receptors. An important pathway involving the beta 3 adrenergic receptor is the cAMP-dependent pathway which influences metabolic functions and energy homeostasis in the body.

The beta 3 adrenergic receptor is associated with obesity and diabetes. Altered function or expression of this receptor can impact the body's ability to regulate energy metabolism leading to these metabolic disorders. The receptor's function has connections with insulin-like growth factor 1 (IGF1) as changes in energy metabolism affect insulin signaling and glucose homeostasis. Abnormal adrenergic receptor activity can also contribute to bladder disorders due to its expression in the urinary system.

style

left-column

style

left-column

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

靶点信息

G protein-coupled receptor for catecholamines that couples to both G(s) and G(i) proteins, leading to either activation or inhibition of adenylate cyclase and cAMP-dependent pathway, respectively (PubMed : 10188996, PubMed : 2570461, PubMed : 8641219). The rank order of potency for physiological agonists is norepinephrine > epinephrine (PubMed : 10188996, PubMed : 2570461, PubMed : 8641219). Involved in the regulation of thermogenesis and lipolysis in brown and white adipose tissue, after coupling to G(s) proteins and stimulation of the cAMP-PKA axis (By similarity). Also activates lipolytic process by coupling to G(i) proteins and consequent initiation of the ERK1/2 MAP kinase cascade (PubMed : 10207024). Participates in relaxation of the blood vessels and the urinary bladder (PubMed : 10188996). Also mediates negative inotropic effects in cardiomyocytes through activation of an NO synthase pathway and subsequent increase in cGMP levels, possibly involving G(i/o) protein-mediated coupling (PubMed : 9769330).

See full target information ADRB3

style

left-column

style

left-column

style

right-column

Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-beta 3 Adrenergic Receptor抗体[EPR25440-151] (BSA and Azide free)