AB300483

重组Anti-beta 3 Adrenergic Receptor抗体[EPR25440-151]

Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151]

20ul selling size
RabMAb
Recombinant
了解详情

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(1 Publication)

Rabbit Recombinant Monoclonal beta 3 Adrenergic Receptor antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

查看别名

ADRB3R, B3AR, ADRB3, Beta-3 adrenergic receptor, Beta-3 adrenoreceptor, Beta-3 adrenoceptor

2 Images

Lab

Western blot - Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151] (AB300483)

Blocking and diluting buffer : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 16177222).

All lanes:

Western blot - Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151] (ab300483) at 1/1000 dilution

Lane 1:

Human fat tissue lysate at 20 µg

Lane 2:

Human spleen (PMID: 11564599) at 40 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

false

Exposure time: 15s

Lab

Western blot - Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151] (AB300483)

Blocking and diluting buffer : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 16177222).

All lanes:

Western blot - Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151] (ab300483) at 1/1000 dilution

All lanes:

Human bladder tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

false

Exposure time: 10s

不同偶联物与剂型 (1)

Carrier free

Anti-beta 3 Adrenergic Receptor antibody [EPR25440-151] (BSA and Azide free)

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR25440-151

亚型

IgG

不含载体蛋白

反应种属

Human

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The beta 3 adrenergic receptor also known as beta-3 adrenoceptor or B3 adrenergic receptor is a member of the adrenergic receptor family that activates the GS protein upon binding ligands like catecholamines. This receptor approximately 408 amino acids in size has a predominant mass of around 45 kDa. It is mainly expressed in adipose tissue but also found in the gallbladder urinary bladder and the gastrointestinal tract. These receptors primarily mediate lipolysis and thermogenesis in brown adipose tissue through interaction with norepinephrine and epinephrine.

Biological function summary

The beta 3 adrenergic receptor plays an important role in energy expenditure and metabolic processes. It does not typically form part of a complex but interacts with GS proteins leading to the activation of adenylyl cyclase and consequent increase in cAMP levels. This receptor is instrumental in the regulation of lipid metabolism which aids in maintaining balance in energy store.

Pathways

The beta 3 adrenergic receptor is involved in the adrenergic signaling pathways regulating energy expenditure and thermogenesis. These pathways often work together with other adrenergic receptors such as beta 1 and beta 2 receptors. An important pathway involving the beta 3 adrenergic receptor is the cAMP-dependent pathway which influences metabolic functions and energy homeostasis in the body.

The beta 3 adrenergic receptor is associated with obesity and diabetes. Altered function or expression of this receptor can impact the body's ability to regulate energy metabolism leading to these metabolic disorders. The receptor's function has connections with insulin-like growth factor 1 (IGF1) as changes in energy metabolism affect insulin signaling and glucose homeostasis. Abnormal adrenergic receptor activity can also contribute to bladder disorders due to its expression in the urinary system.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

G protein-coupled receptor for catecholamines that couples to both G(s) and G(i) proteins, leading to either activation or inhibition of adenylate cyclase and cAMP-dependent pathway, respectively (PubMed : 10188996, PubMed : 2570461, PubMed : 8641219). The rank order of potency for physiological agonists is norepinephrine > epinephrine (PubMed : 10188996, PubMed : 2570461, PubMed : 8641219). Involved in the regulation of thermogenesis and lipolysis in brown and white adipose tissue, after coupling to G(s) proteins and stimulation of the cAMP-PKA axis (By similarity). Also activates lipolytic process by coupling to G(i) proteins and consequent initiation of the ERK1/2 MAP kinase cascade (PubMed : 10207024). Participates in relaxation of the blood vessels and the urinary bladder (PubMed : 10188996). Also mediates negative inotropic effects in cardiomyocytes through activation of an NO synthase pathway and subsequent increase in cGMP levels, possibly involving G(i/o) protein-mediated coupling (PubMed : 9769330).

See full target information ADRB3

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:3824 PubMed39885251

2025

Sympathetic innervation induced by nerve growth factor promotes malignant transformation in gastric cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Takefumi Itami,Yukinori Kurokawa,Takaomi Hagi,Shinnosuke Nagano,Rennosuke Nakamoto,Yu Kamakura,Tsuyoshi Takahashi,Takuro Saito,Kazuyoshi Yamamoto,Kota Momose,Kotaro Yamashita,Koji Tanaka,Tomoki Makino,Kiyokazu Nakajima,Hidetoshi Eguchi,Yuichiro Doki

View all publications

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重组Anti-beta 3 Adrenergic Receptor抗体[EPR25440-151]