Anti-beta 3 Adrenergic Receptor抗体(ab77588)
Key features and details
- Goat polyclonal to beta 3 Adrenergic Receptor
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-beta 3 Adrenergic Receptor抗体
参阅全部 beta 3 Adrenergic Receptor 一抗 -
描述
山羊多克隆抗体to beta 3 Adrenergic Receptor -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Chimpanzee
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免疫原
Synthetic peptide:
PHENSSLAPWPD-C
, corresponding to N terminal amino acids 5-16 of human beta 3 Adrenergic Receptor (NP_000016.1) -
阳性对照
- Human brain (cerebellum, cerebral cortex, frontal cortex) lysates.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
ab77588 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab77588于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
| 说明 |
|---|
应用说明
Peptide ELISA: Antibody detection limit dilution 1/128000.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
靶标
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功能
Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis. -
组织特异性
Expressed mainly in adipose tissues. -
序列相似性
Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 464113 Chimpanzee
- Entrez Gene: 155 Human
- Omim: 109691 Human
- SwissProt: P13945 Human
- Unigene: 2549 Human
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别名
- ADR B3 antibody
- ADRB 3 antibody
- ADRB3 antibody
see all
图片
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Western blot - Anti-beta 3 Adrenergic Receptor antibody (ab77588)Anti-beta 3 Adrenergic Receptor antibody (ab77588) at 0.03 µg/ml + human brain (cerebral cortex) lysate (in RIPA buffer) at 35 µg
Predicted band size: 45 kDa
Observed band size: 45 kDa
Primary incubation was 1 hour.
数据表及文件
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SDS download
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Datasheet download
文献 (2)
ab77588 被引用在 2 文献中.
- Pasha A et al. ß3-Adrenoreceptor Activity Limits Apigenin Efficacy in Ewing Sarcoma Cells: A Dual Approach to Prevent Cell Survival. Int J Mol Sci 20:N/A (2019). PubMed: 31052299
- O'Leary AP et al. Beta-Adrenoceptor Activation Reduces Both Dermal Microvascular Endothelial Cell Migration Via a cAMP-Dependent Mechanism and Wound Angiogenesis. J Cell Physiol N/A:N/A (2014). WB ; Human . PubMed: 24986762