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AB215889

Anti-beta 2 Microglobulin抗体[B2M/961]

Anti-beta 2 Microglobulin antibody [B2M/961]

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(2 Publications)

Mouse Monoclonal beta 2 Microglobulin antibody. Suitable for ICC, Flow Cyt, WB, IHC-P and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human B2M.

查看别名

CDABP0092, HDCMA22P, B2M, Beta-2-microglobulin

8 Images
Immunocytochemistry - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)
  • ICC

Supplier Data

Immunocytochemistry - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunocytochemistry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling beta 2 Microglobulin with ab215889 at 4 μg/mL followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Flow Cytometry analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling beta 2 Microglobulin with ab215889 at 2 μg/106 cells followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human endometrial carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human bladder carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cervical carcinoma tissue labeling beta 2 Microglobulin with ab215889 at 1 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human melanoma tissue labeling beta 2 Microglobulin with ab215889 at 1 μg/ml.

Western blot - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)
  • WB

Supplier Data

Western blot - Anti-beta 2 Microglobulin antibody [B2M/961] (AB215889)

All lanes:

Western blot - Anti-beta 2 Microglobulin antibody [B2M/961] (ab215889) at 2 µg/mL

Lane 1:

THP-1 (human monocytic leukemia cell line) whole cell lysate

Lane 2:

Raji (human Burkitt's lymphoma cell line) whole cell lysate

Predicted band size: 14 kDa

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关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

B2M/961

亚型

IgG2b

轻链类型

kappa

不含载体蛋白

No

反应种属

Human

应用

ICC, WB, Flow Cyt, IHC-P

applications

免疫原

Recombinant Full Length Protein corresponding to Human B2M.

P61769

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替代产品

Primary Antibodies

AB234410

Anti-beta 2 Microglobulin antibody [B2M/1857R]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [B2M/1857R] (AB234410)

  • 0
  • 0
Primary Antibodies

AB234404

Anti-beta 2 Microglobulin antibody [rB2M/961]

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [rB2M/961] (AB234404)

  • 0
  • 0
Primary Antibodies

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Flow Cytometry (Intracellular) - Anti-beta 2 Microglobulin antibody [EPR21752-214] (AB218230)

  • 4
  • 2
Primary Antibodies

AB75853

Anti-beta 2 Microglobulin antibody [EP2978Y]

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Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody [EP2978Y] (AB75853)

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Primary Antibodies

AB214769

Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free

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Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody [EP2978Y] - BSA and Azide free (AB214769)

  • 0
  • 0
Primary Antibodies

AB237032

Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-beta 2 Microglobulin antibody [EPR21752-214] - BSA and Azide free (AB237032)

  • 0
  • 0
Primary Antibodies

AB236274

Anti-beta 2 Microglobulin antibody [rB2M/961] - BSA and Azide free

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta 2 Microglobulin antibody [rB2M/961] - BSA and Azide free (AB236274)

  • 0
  • 0

搭配使用产品

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Anti-HLA Class 1 ABC antibody [EPR22172]

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  • 1
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AB24982

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AB16669

Anti-CD3 epsilon antibody [SP7]

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Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

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Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

  • 0
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "1-4 µg/mL", "ICC-species-notes": "<p></p>", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1-2 µg for 10^6 Cells", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Primary incubation for 30 minutes at room temperature.</p>" }, "Primates": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
纯化说明
ab215889 was purified from Bioreactor Concentrate by Protein A/G.
存储溶液
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.
Biological function summary

Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.

Pathways

Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.

Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed : 25356553).
See full target information B2M
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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

JCI insight 8: PubMed36946463

2023

JAK-STAT activation contributes to cytotoxic T cell-mediated basal cell death in human chronic lung allograft dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Aaditya Khatri,Jamie L Todd,Fran L Kelly,Andrew Nagler,Zhicheng Ji,Vaibhav Jain,Simon G Gregory,Kent J Weinhold,Scott M Palmer

Nature communications 9:3634 PubMed30194383

2018

Mapping molecular landmarks of human skeletal ontogeny and pluripotent stem cell-derived articular chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Gabriel B Ferguson,Ben Van Handel,Maxwell Bay,Petko Fiziev,Tonis Org,Siyoung Lee,Ruzanna Shkhyan,Nicholas W Banks,Mila Scheinberg,Ling Wu,Biagio Saitta,Joseph Elphingstone,A Noelle Larson,Scott M Riester,April D Pyle,Nicholas M Bernthal,Hanna Ka Mikkola,Jason Ernst,Andre J van Wijnen,Michael Bonaguidi,Denis Evseenko
View all publications
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