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Anti-beta 2 Microglobulin抗体[B2M-01] (ab759)

Reviews (1)Q&A (3)References (11)
Western blot - Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)
  • Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)
  • Western blot - Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)

Key features and details

  • Mouse monoclonal [B2M-01] to beta 2 Microglobulin
  • Suitable for: WB, Flow Cyt
  • Reacts with: Human
  • Isotype: IgG2a

Conjugates logo Related conjugates and formulations

Biotin PE PerCP/Cy5.5®

概述

  • 产品名称

    Anti-beta 2 Microglobulin抗体[B2M-01]
    参阅全部 beta 2 Microglobulin 一抗

  • 描述

    小鼠单克隆抗体[B2M-01] to beta 2 Microglobulin

  • 宿主

    Mouse

  • 特异性

    This antibody reacts with beta-2-microglobulin, associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble forms of beta-2-microglobulin. The antibody detected beta-2-microglobulin in U-937 and RAJI cell lines in WB in non reducing conditions however failed to detect a band in JEG-3 and A431 cell lysates. All lysates gave a signal in direct ELISA.

  • 经测试应用

    适用于: WB, Flow Cytmore details

  • 种属反应性

    与反应: Human
    不与反应: Mouse, Rabbit, Chicken, Cow, Dog, Pig

  • 免疫原

    Full length native protein (purified) (Human).

  • 阳性对照

    • U-937 and RAJI cell lysates

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab759于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 2 µg/ml. Use under non reducing condition. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).

Use under non reducing conditions. Incubate overnight. Block with 5%milk for 2hrs.
Flow Cyt
Use a concentration of 0.5 - 4 µg/ml.
说明
WB
Use a concentration of 2 µg/ml. Use under non reducing condition. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).

Use under non reducing conditions. Incubate overnight. Block with 5%milk for 2hrs.
Flow Cyt
Use a concentration of 0.5 - 4 µg/ml.

靶标

  • 功能

    Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system.

  • 疾病相关

    Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
    Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis.

  • 序列相似性

    Belongs to the beta-2-microglobulin family.
    Contains 1 Ig-like C1-type (immunoglobulin-like) domain.

  • 翻译后修饰

    Glycation of Ile-21 is observed in long-term hemodialysis patients.

  • 细胞定位

    Secreted. Detected in serum and urine.
  • Information by UniProt

  • 数据库链接

    • 别名

      • B2M antibody
      • B2MG_HUMAN antibody
      • Beta 2 microglobin antibody
      • Beta 2 microglobulin antibody
      • Beta 2 microglobulin precursor antibody
      • Beta chain of mhc class 1 proteins antibody
      • Beta chain of MHC class I molecules antibody
      • Beta-2-microglobulin form pI 5.3 antibody
      • CDABP0092 antibody
      • Hdcma22p antibody
      • IMD43 antibody
      see all

    图片

    • Western blot - Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)
      Western blot - Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)
      All lanes : Anti-beta 2 Microglobulin antibody [B2M-01] (ab759) at 2 µg/ml

      Lane 1 : Raji cell lysate under non-reducing condition
      Lane 2 : Jurkat cell lysate under non-reducing condition
      Lane 3 : HeLa cell lysate under non-reducing condition
      Lane 4 : Daudi cell lysate under non-reducing condition
      Lane 5 : Raji cell lysate under reducing condition
      Lane 6 : Jurkat cell lysate under reducing condition
      Lane 7 : HeLa cell lysate under reducing condition
      Lane 8 : Daudi cell lysate under reducing condition

      Secondary
      All lanes : IRDye800-conjugated anti-mouse secondary antibody

      Predicted band size: 13 kDa
      Observed band size: 12 kDa why is the actual band size different from the predicted?

    • Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)
      Flow Cytometry - Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)

      Flow cytometric analysis of Human Peripheral Blood cells labelling beta 2 Microglobulin with ab759 at 0.3 ug/ml showing separation of human monocytes (red-filled) from human beta2-Microglobulin negative blood debris (black-dashed).

    • Western blot - Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)
      Western blot - Anti-beta 2 Microglobulin antibody [B2M-01] (ab759)
      All lanes : Anti-beta 2 Microglobulin antibody [B2M-01] (ab759) at 2 µg/ml

      Lane 1 : JEG-3 cell lysate
      Lane 2 : A-431 cell lysate
      Lane 3 : U-937 cell lysate
      Lane 4 : Raji cell lysate

      Developed using the ECL technique.

      Performed under non-reducing conditions.

      Predicted band size: 13 kDa



      Lysates were separated on 15% SDS–PAGE gel and electrotransferred to a PVDF membrane. The membrane was blocked in PBS/Tween buffer containing 5% non fat dry milk for 2 hours and probed with ab759 at 4°C overnight.

    文献 (11)

    发表研究结果有使用 ab759？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab759 被引用在 11 文献中.

    • Rodríguez-Rodríguez M  et al. Activation of Peptidylarginine Deiminase in the Salivary Glands ofBalb/cMice Drives the Citrullination of Ro and La Ribonucleoproteins. J Immunol Res 2017:8959687 (2017). PubMed: 29318161
    • Raha-Chowdhury R  et al. Expression and cellular localization of hepcidin mRNA and protein in normal rat brain. BMC Neurosci 16:24 (2015). WB . PubMed: 25896789
    • Mattheij NJ  et al. Dual mechanism of integrin aIIbß3 closure in procoagulant platelets. J Biol Chem 288:13325-36 (2013). PubMed: 23519467
    • Tiwari S  et al. Divalent cations regulate the folding and activation status of integrins during their intracellular trafficking. J Cell Sci 124:1672-80 (2011). PubMed: 21511727
    • Slany A  et al. Cell characterization by proteome profiling applied to primary hepatocytes and hepatocyte cell lines Hep-G2 and Hep-3B. J Proteome Res 9:6-21 (2010). WB ; Human . PubMed: 19678649
    View all Publications for this product

    客户评价及客户问答

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    提交评价 提交问题

    1-4 of 4 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-beta 2 Microglobulin antibody [B2M-01]

     Average
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Sample
    Mouse Tissue sections (Brain)
    Specification
    Brain
    Permeabilization
    Yes - 0.1M PBS with 3% TritonX100
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Ruma Raha-Chowdhury

    Verified customer

    提交于 Aug 14 2014

    Question

    I am not seeing any band in human endothelial cells. I load 30 ug, tried denatured, reducing conditions, incubated primary overnight at 4 degrees, tried 1/500-1/1000. Do I need to use non-reduced, non-denaturing conditions? Can you send me a picture of the blot you did?

    Read More
    Answer

    Thank you for contacting us for technical support regarding ab759. I have contacted the laboratory and was able to find out that indeed non reducing conditions are advised with this antibody. I have added immediately on our datasheet (accessible below) this information and the image of the blot where four lysates were tested and where you can see a clear band of beta 2 Microglobulin with two lysates (U-937 cell lysate and Raji cell lysate). We expected a signal on Jeg3 cells since on the native lysate it worked by ELISA (ab759 as detecting antibody, directly conjugated with HRP). However, for WB condition, the lysate was not reduced (only denatured by SDS), which may explain the lack of signal with this lysate (furthermore each lysate preparation was not done at the same time). Therefore collectively, the antibody works in western blotting in non reduced conditions and you may find you can achieve a signal with non reducing conditions. May I also suggest to use a 15 or 20% gel to achieve a good separation and run a positive control of U-937 cell lysate or Raji cell lysate? We used a concentration of 2ug/ml of ab729, incubating overnight and blocking for 2hrs with 5% non fat milk. I hope this information will help you, please do not hesitate to contact me again if I can be of further assistance,

    Read More

    Question

    Does this antibody cross-react with MHC class I heavy chain?

    Read More
    Answer

    Thank you for your enquiry. This antibody does not cross react with MHC class I heavy chain.

    Read More

    Question

    I have a customer who urgently needs and anti-beta 2 Microglobulin antibody (for use in Western Blotting). I have been told by many of our suppliers that this clone (B2M-01) has died; and that the product will be on indefinate back order. Have you had any problems with this antibody (AB759)? If not, could you tell me what the availability of it would be? Thanking you in advance for your time and consideration in this matter, I will look forward to your response.

    Read More
    Answer

    We do not have any problems with this clone. We have 3 units that are in stock currently.

    Read More

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