Anti-beta 2 Microglobulin抗体(ab175031)
Key features and details
- Rabbit polyclonal to beta 2 Microglobulin
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG
概述
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产品名称
Anti-beta 2 Microglobulin抗体
参阅全部 beta 2 Microglobulin 一抗 -
描述
兔多克隆抗体to beta 2 Microglobulin -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Mouse, Human, African green monkey -
免疫原
Recombinant full length protein corresponding to Human beta 2 Microglobulin.
Database link: P61769 -
阳性对照
- Jurkat, COS-7, HeLa and K562 cell extracts. Mouse lung, liver and spleen extracts.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 49% PBS -
Concentration information loading... -
纯度
Protein A purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
- HeLa whole cell lysate (ab150035)
- Mouse spleen tissue lysate - total protein (ab29293)
- Mouse lung normal tissue lysate - total protein (ab29297)
- Mouse liver tissue lysate - total protein (ab29301)
- HeLa whole cell lysate (ab29545)
- Jurkat whole cell lysate (ab30128)
- Mouse spleen tissue lysate - total protein (ab7937)
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175031于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC/IF |
Use at an assay dependent concentration.
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| WB |
1/500 - 1/2000. Predicted molecular weight: 14 kDa.
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| 说明 |
|---|
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ICC/IF
Use at an assay dependent concentration. |
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WB
1/500 - 1/2000. Predicted molecular weight: 14 kDa. |
靶标
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功能
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. -
疾病相关
Defects in B2M are the cause of hypercatabolic hypoproteinemia (HYCATHYP) [MIM:241600]. Affected individuals show marked reduction in serum concentrations of immunoglobulin and albumin, probably due to rapid degradation.
Note=Beta-2-microglobulin may adopt the fibrillar configuration of amyloid in certain pathologic states. The capacity to assemble into amyloid fibrils is concentration dependent. Persistently high beta(2)-microglobulin serum levels lead to amyloidosis in patients on long-term hemodialysis. -
序列相似性
Belongs to the beta-2-microglobulin family.
Contains 1 Ig-like C1-type (immunoglobulin-like) domain. -
翻译后修饰
Glycation of Ile-21 is observed in long-term hemodialysis patients. -
细胞定位
Secreted. Detected in serum and urine. - Information by UniProt
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数据库链接
- Entrez Gene: 567 Human
- Entrez Gene: 12010 Mouse
- Omim: 109700 Human
- SwissProt: P61769 Human
- SwissProt: P01887 Mouse
- Unigene: 534255 Human
- Unigene: 702947 Human
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别名
- B2M antibody
- B2MG_HUMAN antibody
- Beta 2 microglobin antibody
see all
图片
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Western blot - Anti-beta 2 Microglobulin antibody (ab175031)All lanes : Anti-beta 2 Microglobulin antibody (ab175031) at 1/500 dilution
Lane 1 : Jurkat cell extract
Lane 2 : COS-7 cell extract
Lane 3 : HeLa cell extract
Lane 4 : K562 cell extract
Lane 5 : Mouse lung extract
Lane 6 : Mouse liver extract
Lane 7 : Mouse spleen extract
Predicted band size: 14 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-beta 2 Microglobulin antibody (ab175031)Immunocytochemistry/Immunofluorescence analysis of MCF7 cells using ab175031. Blue DAPI for nuclear staining.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab175031 被引用在 3 文献中.
- Fels J et al. Cadmium Complexed with ß2-Microglubulin, Albumin and Lipocalin-2 rather than Metallothionein Cause Megalin:Cubilin Dependent Toxicity of the Renal Proximal Tubule. Int J Mol Sci 20:N/A (2019). PubMed: 31091675
- Basu A et al. A Novel S100A8/A9 Induced Fingerprint of Mesenchymal Stem Cells associated with Enhanced Wound Healing. Sci Rep 8:6205 (2018). PubMed: 29670130
- Wang H et al. HGF Gene Modification in Mesenchymal Stem Cells Reduces Radiation-Induced Intestinal Injury by Modulating Immunity. PLoS One 10:e0124420 (2015). IHC . PubMed: 25933295