AB125150

Anti-beta 2 Adrenergic Receptor抗体[6H8]

Name: beta 2 Adrenergic Receptor抗体[6H8] (ab125150)| Abcam中文官网
Brand: Abcam Limited
SKU: AB125150
Price: 4781 CNY
Availability: InStock
Rating: 5 (1 reviews)

Anti-beta 2 Adrenergic Receptor antibody [6H8]

(1 Review)

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Mouse Monoclonal beta 2 Adrenergic Receptor antibody. Suitable for Flow Cyt and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human ADRB2.

查看别名

ADRB2R, B2AR, ADRB2, Beta-2 adrenergic receptor, Beta-2 adrenoreceptor, Beta-2 adrenoceptor

1 Images

Flow Cytometry - Anti-beta 2 Adrenergic Receptor antibody [6H8] (AB125150)

Flow Cyt

Unknown

Flow Cytometry - Anti-beta 2 Adrenergic Receptor antibody [6H8] (AB125150)

Overlay histogram showing SHSY-5Y cells stained with ab125150 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab125150, 0.1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed SHSY-5Y cells used under the same conditions. Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

6H8

亚型

IgG1

不含载体蛋白

反应种属

Human

应用

Flow Cyt

applications

免疫原

Synthetic Peptide within Human ADRB2. The exact immunogen used to generate this antibody is proprietary information.

P07550

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.1-1 µg for 10^6 Cells", "FlowCyt-species-notes": "<p><a href='/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" }, "Guinea pig": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "" } } }

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein G

存储溶液

Constituents: PBS, 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The beta 2 adrenergic receptor (also known as beta-2 adrenergic receptor B2 adrenergic receptor beta-2 receptor) is a transmembrane protein that acts as a receptor for catecholamines like adrenaline and noradrenaline. It belongs to the G protein-coupled receptors (GPCRs) superfamily and is involved in signal transduction by activating adenylate cyclase through the Gs protein. The mass of the beta 2 adrenergic receptor is approximately 46 kDa. This receptor is expressed in various tissues including the lungs skeletal muscle liver and smooth muscle.

Biological function summary

The beta 2 adrenergic receptor is important for mediating the physiological responses to adrenaline and noradrenaline. It plays a significant role in bronchodilation and vasodilation by relaxing airway and vascular smooth muscles. While it does not form a part of any complex its signaling initiates a cascade of intracellular events leading to changes in cellular function. This receptor ensures tissue responsiveness to catecholamines influencing cardiac output metabolic rate and smooth muscle relaxation.

Pathways

This receptor is an important component of the adrenergic signaling pathway and is closely associated with the cAMP-dependent signaling pathway. The activation of the beta 2 adrenergic receptor increases intracellular cAMP levels resulting in the activation of protein kinase A (PKA) and subsequent phosphorylation of various target proteins. The receptor interacts with other proteins within these pathways such as adenylate cyclase and PKA ensuring the propagation of downstream signaling that modulates cellular responses.

The beta 2 adrenergic receptor has a notable connection to asthma and chronic obstructive pulmonary disease (COPD). Its role in bronchodilation makes it a therapeutic target for managing these respiratory conditions. Alterations in the function or expression of the beta 2 adrenergic receptor can exacerbate these diseases. Additionally these disorders demonstrate a link with the muscarinic acetylcholine receptors which also affect airway tone regulation. Understanding the interactions between these receptors is essential for developing therapeutic strategies for respiratory diseases.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine.

See full target information ADRB2

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Abcam Product Promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-beta 2 Adrenergic Receptor抗体[6H8]

Anti-beta 2 Adrenergic Receptor antibody [6H8]

Flow Cytometry - Anti-beta 2 Adrenergic Receptor antibody [6H8] (AB125150)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原