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Neuroscience Cell Adhesion Proteins ECM Proteins

Anti-Bestrophin/BEST1抗体[E6-6] (ab2182)

  • Datasheet
  • SDS
Reviews (7)Q&A (1)References (25)

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Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)
  • Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)

Key features and details

  • Mouse monoclonal [E6-6] to Bestrophin/BEST1
  • Suitable for: WB, IHC-Fr, ICC/IF, IP
  • Reacts with: Cow, Dog, Human, Pig, Monkey
  • Isotype: IgG1

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概述

  • 产品名称

    Anti-Bestrophin/BEST1抗体[E6-6]
    参阅全部 Bestrophin/BEST1 一抗
  • 描述

    小鼠单克隆抗体[E6-6] to Bestrophin/BEST1
  • 宿主

    Mouse
  • 经测试应用

    适用于: WB, IHC-Fr, ICC/IF, IPmore details
  • 种属反应性

    与反应: Cow, Dog, Human, Pig, Monkey
    预测可用于: Non human primates不与反应: Mouse, Rat, Rabbit, Goat
  • 免疫原

    Synthetic peptide corresponding to Human Bestrophin/BEST1 aa 568-585 (C terminal) conjugated to keyhole limpet haemocyanin.
    Sequence:

    KDHMDPYWALENRDEAHS


    Database link: O76090
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • IHC-Fr: Pig retinal pigment epithelium tissue. ICC/IF: Bovine retinal pigment epithelium (RPE). WB: Human RPE cell lysate.
  • 常规说明

    This product has switched from ascites to TCS on 9th September 2020.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • 存储溶液

    pH: 7.40
    Preservative: 0.1% Sodium azide
    Constituent: Ascites
  • Concentration information loading...
  • 纯度

    Protein A/G purified
  • 克隆

    单克隆
  • 克隆编号

    E6-6
  • 骨髓瘤

    unknown
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Neuroscience
    • Cell Adhesion Proteins
    • ECM Proteins
    • Neuroscience
    • Sensory System
    • Visual system
    • Neuroscience
    • Processes

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
  • Recombinant Protein

    • Recombinant Human Bestrophin/BEST1 protein (ab152797)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab2182于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (3)
1/1000.
IHC-Fr (2)
Use at an assay dependent concentration.
ICC/IF (1)
Use at an assay dependent concentration.
IP (1)
Use at an assay dependent concentration.
说明
WB
1/1000.
IHC-Fr
Use at an assay dependent concentration.
ICC/IF
Use at an assay dependent concentration.
IP
Use at an assay dependent concentration.

靶标

  • 功能

    Forms calcium-sensitive chloride channels. Highly permeable to bicarbonate.
  • 组织特异性

    Predominantly expressed in the basolateral membrane of the retinal pigment epithelium.
  • 疾病相关

    Defects in BEST1 are the cause of vitelliform macular dystrophy type 2 (VMD2) [MIM:153700]; also known as Best macular dystrophy (BMD). VMD2 is an autosomal dominant form of macular degeneration that usually begins in childhood or adolescence. VMD2 is characterized by typical 'egg-yolk' macular lesions due to abnormal accumulation of lipofuscin within and beneath the retinal pigment epithelium cells. Progression of the disease leads to destruction of the retinal pigment epithelium and vision loss.
    Defects in BEST1 are the cause of retinitis pigmentosa type 50 (RP50) [MIM:613194]. A retinal dystrophy belonging to the group of pigmentary retinopathies. RP is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
    Defects in BEST1 are a cause of adult-onset vitelliform macular dystrophy (AVMD) [MIM:608161]. AVMD is a rare autosomal dominant disorder with incomplete penetrance and highly variable expression. Patients usually become symptomatic in the fourth or fifth decade of life with a protracted disease of decreased visual acuity.
    Defects in BEST1 are the cause of bestrophinopathy autosomal recessive (ARB) [MIM:611809]. A retinopathy characterized by central visual loss, an absent electro-oculogram light rise, and a reduced electroretinogram.
    Defects in BEST1 are the cause of vitreoretinochoroidopathy autosomal dominant (ADVIRC) [MIM:193220]. A disorder characterized by vitreoretinochoroidal dystrophy. The clinical presentation is variable and may be associated with cataract, nanophthalmos, microcornea, shallow anterior chamber, and glaucoma.
  • 序列相似性

    Belongs to the bestrophin family.
  • 翻译后修饰

    Phosphorylated by PP2A.
  • 细胞定位

    Cell membrane. Basolateral cell membrane.
  • Target information above from: UniProt accession O76090 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 7439 Human
    • Omim: 607854 Human
    • SwissProt: O76090 Human
    • SwissProt: Q8WMR7 Pig
    • Unigene: 524910 Human
    • Unigene: 712676 Human
    • 别名

      • ARB antibody
      • BEST 1 antibody
      • BEST antibody
      • Best disease antibody
      • Best macular dystrophy antibody
      • BEST1 antibody
      • BEST1_HUMAN antibody
      • Best1V1Delta2 antibody
      • Bestrophin 1 antibody
      • Bestrophin-1 antibody
      • Bestrophin1 antibody
      • BMD antibody
      • mBest1 antibody
      • RP50 antibody
      • TU15B antibody
      • Vitelliform macular dystrophy 2 antibody
      • Vitelliform macular dystrophy antibody
      • Vitelliform macular dystrophy protein 2 antibody
      • VMD 2 antibody
      • VMD2 antibody
      see all

    图片

    • Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)
      Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)
      Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182) at 1/1000 dilution + Human RPE cell lysate
    • Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)
      Western blot - Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182)This image is courtesy of an Abreview submitted by Dr Vladimir Milenkovic
      All lanes : Anti-Bestrophin/BEST1 antibody [E6-6] (ab2182) at 1/1000 dilution

      Lane 1 : Human RPE, retinal pigment epithelial cell lysate
      Lane 2 : Non transfected HEK 293 cell extract

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-mouse

      Developed using the ECL technique.

      Performed under reducing conditions.

      Observed band size: 67 kDa why is the actual band size different from the predicted?


      Exposure time: 5 minutes


      The primary antobody was diluted in PBS/Tween/5%Milk and incubated for 1.5 hours at 25°C.

      See Abreview

    实验方案

    • Immunohistochemistry protocols
    • Western blot protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (25)

    发表研究结果有使用 ab2182?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2182 被引用在 25 文献中.

    • Shahriari F  et al. MicroRNA profiling reveals important functions of miR-125b and let-7a during human retinal pigment epithelial cell differentiation. Exp Eye Res 190:107883 (2020). PubMed: 31758976
    • Pan T  et al. Combined Transplantation With Human Mesenchymal Stem Cells Improves Retinal Rescue Effect of Human Fetal RPE Cells in Retinal Degeneration Mouse Model. Invest Ophthalmol Vis Sci 61:9 (2020). PubMed: 32639552
    • Ou R  et al. HPV16 E6 oncoprotein-induced upregulation of lncRNA GABPB1-AS1 facilitates cervical cancer progression by regulating miR-519e-5p/Notch2 axis. FASEB J 34:13211-13223 (2020). PubMed: 32844486
    • Hazim RA  et al. Rapid differentiation of the human RPE cell line, ARPE-19, induced by nicotinamide. Exp Eye Res 179:18-24 (2019). PubMed: 30336127
    • Shen H  et al. A novel xeno-free culture system for human retinal pigment epithelium cells. Int J Ophthalmol 12:563-570 (2019). PubMed: 31024807
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-8 of 8 Abreviews or Q&A

    Immunohistochemistry (Frozen sections) abreview for Anti-Bestrophin/BEST1 antibody [E6-6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (Eye, retinal pigment epithelium)
    Permeabilization
    Yes - 0.5% TX100
    Specification
    Eye, retinal pigment epithelium
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    HERR DR. Vladimir Milenkovic

    Verified customer

    提交于 May 29 2008

    Western blot abreview for Anti-Bestrophin/BEST1 antibody [E6-6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Pig Cell lysate - other (Membrane fraction of the pig RPE cells)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    25 µg
    Specification
    Membrane fraction of the pig RPE cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    HERR DR. Vladimir Milenkovic

    Verified customer

    提交于 Feb 25 2008

    Western blot abreview for Anti-Bestrophin/BEST1 antibody [E6-6]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Cow Cell lysate - whole cell (Retinal Pigment Epithelium cells)
    Gel Running Conditions
    Reduced Denaturing (10)
    Loading amount
    25 µg
    Specification
    Retinal Pigment Epithelium cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    HERR DR. Vladimir Milenkovic

    Verified customer

    提交于 Feb 18 2008

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Bestrophin/BEST1 antibody [E6-6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Cow Cell (Bovine Retinal Pigment Epithelium)
    Permeabilization
    Yes - 0.5% TX100
    Specification
    Bovine Retinal Pigment Epithelium
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    HERR DR. Vladimir Milenkovic

    Verified customer

    提交于 Feb 18 2008

    Western blot abreview for Anti-Bestrophin/BEST1 antibody [E6-6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (RPE, retinal pigment epithelial cells)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    RPE, retinal pigment epithelial cells
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    HERR DR. Vladimir Milenkovic

    Verified customer

    提交于 Jan 09 2008

    Immunohistochemistry (Frozen sections) abreview for Anti-Bestrophin/BEST1 antibody [E6-6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Pig Tissue sections (Retinal pigment epithelium)
    Permeabilization
    Yes - 0.5%TX100, 5% goat serum, 1XPBS
    Specification
    Retinal pigment epithelium
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    HERR DR. Vladimir Milenkovic

    Verified customer

    提交于 Jan 08 2008

    Immunoprecipitation abreview for Anti-Bestrophin/BEST1 antibody [E6-6]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (Expression of human Bestrophin 1in HEK 293 cells)
    Total protein in input
    20 µg
    Immuno-precipitation step
    Protein G
    Specification
    Expression of human Bestrophin 1in HEK 293 cells
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    HERR DR. Vladimir Milenkovic

    Verified customer

    提交于 Jan 07 2008

    Question

    Hi, I've been trying to perform IHC on dog retinas using this Bestrophin Ab and am not having much success. The Ab works fine when using OCT embedded formaldehyde fixed retinas, but fails on Bouin fixed, paraffin embedded retinas. Are there any suggestions for a protocol to used with eyes fixed in this manner?

    Read More

    Abcam community

    Verified customer

    Asked on Apr 12 2006

    Answer

    Thank you for your enquiry. Ab2182 has not previously been tested before for application in IHC so we don't have a protocol specific for this antibody. As you're having difficulty with the Bouin’s fixative, you may want to try formalin fixed paraffin-embedded tissue sections. We have a nice general protocol for this that can found under the protocols section of the online datasheet for ab2182. Also, I did find some information on the IHCWorld website that "Bouin’s contains picric acid and any unbound picric acid left in the tissue will prevent adequate staining. It needs to be removed usually by washing the tissue well in running water before processing." You can also wash with 70% EtOH prior to processing. Otherwise, the acid will continue to negatively affect the tissue. Increased fixation does reduce permeability so you may need to reduce the fixation period. I hope this helps. I also encourage you to submit an Abreview for this antibody. We always encourage customers to feed their results back to us, whether positive or negative, and make all information about each of our products available to researchers. If you send us an Abreview, we can offer you 50 Abcam Points (a further 100 Abpoints will be offered for an image). To find out more about our Loyalty Scheme and Abpoints, please visit our website and look at Your Account panel (left bottom section on our home page) or the Abreviews section on the product datasheet. You can submit the Abreview if you click on the Abreview section (top menu bar) on the on-line datasheet of this antibody. It is a very straightforward process. Should you need any help how to submit an Abreview, then please do not hesitate to contact us again. Please contact us again if you have any additional questions.

    Read More

    Abcam Scientific Support

    回复于 Apr 13 2006

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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