AB302670

重组Anti-Beclin-1抗体[EPR19662] -鸡IgY (Chimeric) - BSA and Azide free

Anti-Beclin-1 antibody [EPR19662] - Chicken IgY (Chimeric) - BSA and Azide free

Recombinant
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(1 Publication)

Chicken Recombinant Monoclonal Beclin 1 antibody. Carrier free. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

查看别名

GT197, BECN1, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, Protein GT197

1 Images

Supplier Data

Western blot - Anti-Beclin-1 antibody [EPR19662] - Chicken IgY (Chimeric) - BSA and Azide free (AB302670)

This data was developed using ab302669, the same antibody clone in a different buffer formulation. Blocking / Diluting buffer : 5% NFDM/TBST Exposure time : 3.25 seconds

All lanes:

Western blot - Anti-Beclin-1 antibody [EPR19662] - Chicken IgY (Chimeric) (<a href='/products/primary-antibodies/beclin-1-antibody-epr19662-chicken-igy-chimeric-ab302669'>ab302669</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HepG2 (human hepatocellar carcinoma epithelial cell), whole cell lysate 20 at 20 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 4:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 6:

A20 (mouse reticum sarcoma B lymphocyte), whole cell lysate 20 at 20 µg

Lane 7:

C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg

Lane 8:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (<a href='/products/secondary-antibodies/goat-chicken-igy-h-l-hrp-ab6877'>ab6877</a>) at 1/20000 dilution

Observed band size: 52 kDa

false

Exposure time: 3.25s

不同偶联物与剂型 (1)

Unconjugated

Anti-Beclin-1 antibody [EPR19662] - Chicken IgY (Chimeric)

关键信息

宿主种属

Chicken

克隆

Monoclonal

克隆号

EPR19662

亚型

IgY

不含载体蛋白

Yes

反应种属

Human, Mouse, Rat

应用

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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产品详情

ab302670 is the carrier-free version of ab302669.

This chicken monoclonal chimeric antibody has been engineered from a RabMAb parent antibody (ab207612). By necessity, some rabbit sequence is retained as part of the variable domain. When multiplexing with other rabbit-derived antibodies, using cross absorbed Fc-reactive secondary antibodies are recommended.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

储存信息

Do Not Freeze

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Beclin 1 sometimes referred to as beclin-1 or becn1 is a protein that plays a mechanical role in autophagy. This process involves the recycling of cellular components and is essential for maintaining cellular health. Beclin 1 is approximately 60 kDa in molecular weight and is expressed in a variety of tissues throughout the body. It acts at the molecular level engaging in the initial stages of autophagosome formation which are critical for cell survival during nutrient deprivation.

Biological function summary

The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.

Pathways

Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.

Beclin 1 shows significant relevance in cancer and neurodegenerative diseases. Abnormal Beclin 1 expression and function are linked to a range of cancers as its dysregulation affects apoptosis and autophagic turnover. Furthermore in neurodegenerative disorders like Alzheimer's disease altered Beclin 1 activity can disrupt normal protein degradation exacerbating protein aggregation a hallmark of these conditions. Interactions with proteins like Bcl-2 are essential in the context of these diseases as Bcl-2 modulates the autophagic and apoptotic balance directly impacting disease progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a central role in autophagy (PubMed : 18570871, PubMed : 21358617, PubMed : 23184933, PubMed : 23974797, PubMed : 25484083, PubMed : 28445460, PubMed : 37776275). Acts as a core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways : PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abscission step in cytokinesis, probably in the context of PI3KC3-C2 (PubMed : 20208530, PubMed : 20643123, PubMed : 23974797, PubMed : 26783301). Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis (PubMed : 25275521). May play a role in antiviral host defense.. Beclin-1-C 35 kDa localized to mitochondria can promote apoptosis; it induces the mitochondrial translocation of BAX and the release of proapoptotic factors.. (Microbial infection) Protects against infection by a neurovirulent strain of Sindbis virus.

See full target information BECN1

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in microbiology 16:1631082 PubMed40969437

2025

Dasatinib and Quercetin alleviate type 2 diabetic osteoporosis by regulating serum metabolite and gut microbiome.

Applications

Unspecified application

Species

Unspecified reactive species

Junzheng Yang,Hua Zhang,Zhuoxu Gu,Guanghui Zhou,Guihong Liang,Lingfeng Zeng,Jinlong Zhao,Weiyi Yang,Jun Liu,Jianke Pan

View all publications

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重组Anti-Beclin-1抗体[EPR19662] -鸡IgY (Chimeric) - BSA and Azide free