Anti-Beclin 1抗体(ab62557)
Key features and details
- Rabbit polyclonal to Beclin 1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Beclin 1抗体
参阅全部 Beclin 1 一抗 -
描述
兔多克隆抗体to Beclin 1 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Beclin 1 (N terminal). ab62557 was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Beclin-1. The immunogen is located within amino acids 40 - 90 of Beclin-1.
Database link: Q14457 -
阳性对照
- WB: HEK-293, A549, HeLa, HepG2, K562, MCF7 and SK-N-SH cell lines. Mouse heart, brain and colon tissue. Rat liver, lung, brain and colon tissue. IHC-P: Human brain tissue, mouse kidney and rat liver and spleen tissues. IF: Mouse brain. ICC/IF: HeLa cells.
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常规说明
For Western blot protocol see the applications section.
For information on sample preparation for western blot, please refer here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62557于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P | (2) |
Use a concentration of 2 µg/ml.
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| WB | (4) |
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa).
After transfer, wash membrane in TBST. Incubate membrane with 5% non-fat dry milk in TBS, 4C, overnight.. Dilute ab62557 to recommended dilution in 10mL of blocking buffer. Incubate membrane with ab62557 for 1 h at room temperature. Wash 3 times x 10 min in washing buffer with gentle agitation. Dilute 1μL secondary in 10mL of blocking buffer to make a 1:10,000 dilution (can vary from 1:2,000 to 1:10,000). Incubate membrane for 1 h at rt. Wash 3 x 10 min in washing buffer with gentle agitation. Running Buffer: 25mM Tris, 192 mM Glycine, 0.1% SDS. Transfer Buffer: 20mM Tris, 150mM Glycine, 20% Methanol, 0.038% SDS. Blocking Buffer: 5% non-fat dry milk, TBS. Wash Buffer (TBST): |
| IF |
Use a concentration of 20 µg/ml.
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| ICC/IF | (1) |
1/100.
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| 说明 |
|---|
|
IHC-P
Use a concentration of 2 µg/ml. |
|
WB
Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 52 kDa). After transfer, wash membrane in TBST. Incubate membrane with 5% non-fat dry milk in TBS, 4C, overnight.. Dilute ab62557 to recommended dilution in 10mL of blocking buffer. Incubate membrane with ab62557 for 1 h at room temperature. Wash 3 times x 10 min in washing buffer with gentle agitation. Dilute 1μL secondary in 10mL of blocking buffer to make a 1:10,000 dilution (can vary from 1:2,000 to 1:10,000). Incubate membrane for 1 h at rt. Wash 3 x 10 min in washing buffer with gentle agitation. Running Buffer: 25mM Tris, 192 mM Glycine, 0.1% SDS. Transfer Buffer: 20mM Tris, 150mM Glycine, 20% Methanol, 0.038% SDS. Blocking Buffer: 5% non-fat dry milk, TBS. Wash Buffer (TBST): |
|
IF
Use a concentration of 20 µg/ml. |
|
ICC/IF
1/100. |
靶标
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功能
Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the beclin family. -
细胞定位
Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. - Information by UniProt
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数据库链接
- Entrez Gene: 8678 Human
- Entrez Gene: 56208 Mouse
- Entrez Gene: 114558 Rat
- Omim: 604378 Human
- SwissProt: Q14457 Human
- SwissProt: O88597 Mouse
- SwissProt: Q91XJ1 Rat
- Unigene: 716464 Human
see all -
别名
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
see all
图片
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Western blot - Anti-Beclin 1 antibody (ab62557)All lanes : Anti-Beclin 1 antibody (ab62557) at 1 µg/ml
Lane 1 : 293 human cell line
Lane 2 : A549 human cell line
Lane 3 : HeLa cell line
Lane 4 : HepG2 cell line
Lane 5 : K562 cell line
Lane 6 : MCF7 cell line
Lane 7 : SK-N-SH cell line
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/1000 dilution
Predicted band size: 52 kDaGel: 10%
Running condition: 130V, 1.5hrs
Transfer condition: Wet, 250mA, 2 hrs (NC membrane)
Blocking condition: 5% non-fat milk in TBS at 4 overnight
Primary antibody incubation: RT, 1hr
Secondary antibody incubation: RT, 1hr
Washing condition: 5 ml TBST, 4 x 5min
Exposure system: GE ECL 10 sec
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab62557)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Beclin 1 with ab6257 at 1 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0, microwave, for 20min. Streptavidin-HRP Kit (Rabbit IgG) was used as the secondary antibody.
blocking condition: 5% BSA at Room Temperature for 1 hour
Chromagen system: DAB
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Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (ab62557)Immunofluorescence analysis of HeLa cells labelling Beclin 1 with ab62557 at 1/100 dilution. Cells were fixed with 4% paraformaldehyde at room temperature for 15 minutes and was permeabilized with 0.1% Triton X-100 at room temperature for 4 minutes. Rabbit IgG antibody (Alexa Fluor 488) at 1/2000 was used as the secondary antibody (green). Nuclear DNA was labelled with DAPI (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab62557)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling Beclin 1 with ab6257 at 1 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0, microwave, for 20min. Streptavidin-HRP Kit (Rabbit IgG) was used as the secondary antibody.
blocking condition: 5% BSA at Room Temperature for 1 hour
Chromagen system: DAB
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Western blot - Anti-Beclin 1 antibody (ab62557)All lanes : Anti-Beclin 1 antibody (ab62557) at 1 µg/ml
Lane 1 : Mouse heart tissue
Lane 2 : Mouse brain tissue
Lane 3 : Mouse colon tissue
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 52 kDaGel: 10%
Running condition: 130V, 1.5hrs
Transfer condition: Wet, 250mA, 2 hrs (NC membrane)
Blocking condition: 5% non-fat milk in TBS at 4 overnight
Primary antibody incubation: RT, 1hr
Secondary antibody incubation: RT, 1hr
Washing condition: 5 ml TBST, 4 x 5min
Exposure system: GE ECL 10 sec
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Western blot - Anti-Beclin 1 antibody (ab62557)All lanes : Anti-Beclin 1 antibody (ab62557) at 1 µg
Lane 1 : Rat liver tissue
Lane 2 : Rat heart tissue
Lane 3 : Rat lung tissue
Lane 4 : Rat brain tissue
Lane 5 : Rat ovary tissue
Lane 6 : Rat colon tissue
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 52 kDaGel: 10%
Running condition: 130V, 1.5hrs
Transfer condition: Wet, 250mA, 2 hrs (NC membrane)
Blocking condition: 5% non-fat milk in TBS at 4 overnight
Primary antibody incubation: RT, 1hr
Secondary antibody incubation: RT, 1hr
Washing condition: 5 ml TBST, 4 x 5min
Exposure system: GE ECL 10 sec
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab62557)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue labelling Beclin 1 with ab6257 at 1 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0, microwave, for 20min. Streptavidin-HRP Kit (Rabbit IgG) was used as the secondary antibody.
blocking condition: 5% BSA at Room Temperature for 1 hour
Chromagen system: DAB
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Immunofluorescence - Anti-Beclin 1 antibody (ab62557)ab62557 at 20 µg/mL staining Beclin 1 (green) in mouse brain tissue by Immunofluorescence. DAPI staining in blue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab62557)Paraffin-embedded human brain tissue stained for Beclin 1 using ab62557 at 2.5 µg/ml in immunohistochemical analysis.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (291)
ab62557 被引用在 291 文献中.
- Liu R et al. Alpha-ketoglutarate up-regulates autophagic activity in peri-implant environment and enhances dental implant osseointegration in osteoporotic mice. J Clin Periodontol 50:671-683 (2023). PubMed: 36734077
- Abdel-Hamid HA et al. Hemin protects against cell stress induced by estrogen and progesterone in rat mammary glands via modulation of Nrf2/HO-1 and NF-κB pathways. Cell Stress Chaperones 28:289-301 (2023). PubMed: 36930344
- Qiu Y et al. Liquiritigenin promotes osteogenic differentiation and prevents bone loss via inducing auto-lysosomal degradation and inhibiting apoptosis. Genes Dis 10:284-300 (2023). PubMed: 37013063
- Jin Y et al. Effect of FTY-720 on Pulmonary Fibrosis in Mice via the TGF-β1 Signaling Pathway and Autophagy. Biomol Ther (Seoul) 31:434-445 (2023). PubMed: 37019836
- Ou C et al. CRTC2 promotes paclitaxel resistance by inducing autophagy in ovarian cancer in part via the PI3K-AKT signaling axis. J Cancer 14:1011-1023 (2023). PubMed: 37151390