Anti-Beclin 1抗体(ab223348)
Key features and details
- Rabbit polyclonal to Beclin 1
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-Beclin 1抗体
参阅全部 Beclin 1 一抗 -
描述
兔多克隆抗体to Beclin 1 -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WBmore details -
种属反应性
与反应: Rat, Human -
免疫原
Synthetic peptide corresponding to Human Beclin 1 (internal sequence).
Database link: Q14457 -
阳性对照
- WB: Rat liver lysate; HeLa cell lysate. ICC/IF: SK-N-BE cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab223348于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
1/100.
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WB |
1/1000. Predicted molecular weight: 52 kDa.
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说明 |
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ICC/IF
1/100. |
WB
1/1000. Predicted molecular weight: 52 kDa. |
靶标
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功能
Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus. -
组织特异性
Ubiquitous. -
序列相似性
Belongs to the beclin family. -
细胞定位
Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells. - Information by UniProt
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数据库链接
- Entrez Gene: 8678 Human
- Entrez Gene: 114558 Rat
- Omim: 604378 Human
- SwissProt: Q14457 Human
- SwissProt: Q91XJ1 Rat
- Unigene: 716464 Human
- Unigene: 2776 Rat
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别名
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
see all
图片
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4% formaldehyde-fixed SK-N-BE cells stained for Beclin 1 (green) using ab223348 at 1/100 dilution in ICC/IF. Secondary: Goat Anti-Rabbit ATTO 488 at 1/100 for 60 minutes at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 minutes RT, 5 minutes RT.
(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223348 (D) Merge.
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Anti-Beclin 1 antibody (ab223348) at 1/1000 dilution + HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 20 µg
Secondary
Goat Anti-Rabbit: HRP at 1/2000 dilution
Predicted band size: 52 kDaBlocking: 5% milk + TBST for 1 hour at RT.
Color Development: TMB solution for 12 minutes at RT.
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Anti-Beclin 1 antibody (ab223348) at 1/1000 dilution + Rat liver lysate at 20 µg
Secondary
Goat Anti-Rabbit: HRP at 1/2000 dilution
Predicted band size: 52 kDaBlocking: 5% milk + TBST for 1 hour at RT.
Color Development: TMB solution for 12 minutes at RT.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (1)
ab223348 被引用在 1 文献中.
- Zhang B et al. ClC-3 induction protects against cerebral ischemia/reperfusion injury through promoting Beclin1/Vps34-mediated autophagy. Hum Cell 33:1046-1055 (2020). PubMed: 32772229