AB324112

Anti-BDNF antibody [EPR29334-131] - BSA and Azide free (Detector)

RabMAb
Recombinant
了解详情

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Rabbit Recombinant Monoclonal BDNF antibody. Carrier free. Suitable for sELISA and reacts with Human, Rat samples.

Neurotrophic factor BDNF precursor form, proBDNF, Brain-derived neurotrophic factor, Bdnf

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Sandwich ELISA - Anti-BDNF antibody [EPR29334-131] - BSA and Azide free (Detector) (AB324112)

sELISA

Supplier Data

Sandwich ELISA - Anti-BDNF antibody [EPR29334-131] - BSA and Azide free (Detector) (AB324112)

Interpolated concentration of native BDNF was measured at different sample concentrations. Undiluted samples are as follows : human serum 100%, and human plasma (Citrate) 100%. Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

sELISA

Supplier Data

Sandwich ELISA - Anti-BDNF antibody [EPR29334-131] - BSA and Azide free (Detector) (AB324112)

Sandwich ELISA using Rat BDNF immunogen protein standard curve with the capture antibody ab324065 at 2 ug/mL and detector antibody ab324112 at 0.5 ug/mL. Background-subtracted absorbance data values (mean +/- standard deviation are shown.)

sELISA

Supplier Data

Sandwich ELISA - Anti-BDNF antibody [EPR29334-131] - BSA and Azide free (Detector) (AB324112)

Interpolated concentration of native BDNF was measured at different sample concentrations. Undiluted samples are as follows : rat serum 100%, and rat plasma 100%. Sample dilutions are made in antibody diluent CP2 (ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR29334-131

亚型

IgG

不含载体蛋白

Yes

反应种属

Human, Rat

应用

sELISA

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Rat": { "sELISA-species-checked": "testedAndGuaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }

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产品详情

ab324112 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Rat BDNF. The recommended pair for sandwich ELISA is:

Capture: ab324065, Human/Rat BDNF Capture Antibody (unconjugated)

Detector: ab324112, Human/Rat BDNF Detector Antibody (unconjugated)

The reference range value is 15.6-1000 pg/mL.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification

存储溶液

pH: 7.2 - 7.4 Constituents: PBS

运输条件

Blue Ice

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Important signaling molecule that activates signaling cascades downstream of NTRK2 (By similarity). During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability (By similarity).. Neurotrophic factor BDNF precursor form. Important signaling molecule that activates signaling cascades downstream of NTRK2. Activates signaling cascades via the heterodimeric receptor formed by NGFR and SORCS2. Signaling via NGFR and SORCS2 plays a role in synaptic plasticity and long-term depression (LTD). Binding to NGFR and SORCS2 promotes neuronal apoptosis. Promotes neuronal growth cone collapse.

See full target information Bdnf

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Anti-BDNF antibody [EPR29334-131] - BSA and Azide free (Detector)