Anti-BCRP/ABCG2抗体[1H2] (ab130244)
Key features and details
- Mouse monoclonal [1H2] to BCRP/ABCG2
- Suitable for: WB, ELISA, ICC/IF
- Reacts with: Mouse, Human, African green monkey
- Isotype: IgG1
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-BCRP/ABCG2抗体[1H2]
参阅全部 BCRP/ABCG2 一抗 -
描述
小鼠单克隆抗体[1H2] to BCRP/ABCG2 -
宿主
Mouse -
经测试应用
适用于: WB, ELISA, ICC/IFmore details -
种属反应性
与反应: Mouse, Human, African green monkey -
免疫原
Purified recombinant fragment of Human BCRP/ABCG2 expressed in E. coli.
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阳性对照
- HepG2, COS7 and NIH/3T3 cell lysates; HeLa cells.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituent: 99.99% PBS -
Concentration information loading...
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纯度
Tissue culture supernatant -
克隆
单克隆 -
克隆编号
1H2 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab130244于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 72 kDa.
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ELISA |
1/10000.
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ICC/IF |
1/200 - 1/1000.
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说明 |
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WB
1/500 - 1/2000. Predicted molecular weight: 72 kDa. |
ELISA
1/10000. |
ICC/IF
1/200 - 1/1000. |
靶标
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功能
Xenobiotic transporter that may play an important role in the exclusion of xenobiotics from the brain. May be involved in brain-to-blood efflux. Appears to play a major role in the multidrug resistance phenotype of several cancer cell lines. When overexpressed, the transfected cells become resistant to mitoxantrone, daunorubicin and doxorubicin, display diminished intracellular accumulation of daunorubicin, and manifest an ATP-dependent increase in the efflux of rhodamine 123. -
组织特异性
Highly expressed in placenta. Low expression in small intestine, liver and colon. -
序列相似性
Belongs to the ABC transporter superfamily. ABCG family. Eye pigment precursor importer (TC 3.A.1.204) subfamily.
Contains 1 ABC transmembrane type-2 domain.
Contains 1 ABC transporter domain. -
翻译后修饰
Glycosylation-deficient ABCG2 is normally expressed and functional. -
细胞定位
Cell membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 9429 Human
- Entrez Gene: 26357 Mouse
- Omim: 603756 Human
- SwissProt: Q9UNQ0 Human
- SwissProt: Q7TMS5 Mouse
- Unigene: 480218 Human
- Unigene: 333096 Mouse
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别名
- ABC transporter antibody
- ABC15 antibody
- ABCG 2 antibody
see all
图片
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All lanes : Anti-BCRP/ABCG2 antibody [1H2] (ab130244) at 1/500 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : COS7 cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : NIH/3T3 cell lysate
Predicted band size: 72 kDa -
ab130244, at 1/200 dilution, staining BCRP/ABCG2 in HeLa cells by Immunofluorescence (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
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ELISA using ab130244.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (9)
ab130244 被引用在 9 文献中.
- Xu P et al. Trigeminal nerve-derived substance P regulates limbal stem cells by the PI3K-AKT pathway. iScience 26:106688 (2023). PubMed: 37187698
- Bai Y et al. Hydrogen alleviated cognitive impairment and blood‒brain barrier damage in sepsis-associated encephalopathy by regulating ABC efflux transporters in a PPARα-dependent manner. BMC Neurosci 24:37 (2023). PubMed: 37474902
- Yang H et al. FBI-1 enhanced the resistance of triple-negative breast cancer cells to chemotherapeutic agents via the miR-30c/PXR axis. Cell Death Dis 11:851 (2020). PubMed: 33051436
- Ke B et al. Interleukin-7 Resensitizes Non-Small-Cell Lung Cancer to Cisplatin via Inhibition of ABCG2. Mediators Inflamm 2019:7241418 (2019). PubMed: 31915416
- Cheng CC et al. Stat3/Oct-4/c-Myc signal circuit for regulating stemness-mediated doxorubicin resistance of triple-negative breast cancer cells and inhibitory effects of WP1066. Int J Oncol 53:339-348 (2018). PubMed: 29750424
- Wang P et al. HIF1a regulates single differentiated glioma cell dedifferentiation to stem-like cell phenotypes with high tumorigenic potential under hypoxia. Oncotarget 8:28074-28092 (2017). PubMed: 28427209
- Park SJ et al. Estradiol, TGF-ß1 and hypoxia promote breast cancer stemness and EMT-mediated breast cancer migration. Oncol Lett 11:1895-1902 (2016). PubMed: 26998096
- Topalis D et al. Resistance to the nucleotide analogue cidofovir in HPV(+) cells: a multifactorial process involving UMP/CMP kinase 1. Oncotarget 7:10386-401 (2016). WB . PubMed: 26824416
- Wang C et al. Associations between ABCG2 gene polymorphisms and isolated septal defects in a Han Chinese population. DNA Cell Biol 33:689-98 (2014). PubMed: 24979295