AB229193

Anti-BCRP/ABCG2 抗体 [EPR21122]

Anti-BCRP/ABCG2 antibody [EPR21122]

RabMAb
Recombinant
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(8 Publications)

Rabbit Recombinant Monoclonal BCRP/ABCG2 antibody. Suitable for Flow Cyt, ICC/IF, IHC-P and reacts with Human samples. Cited in 8 publications.

查看别名

CD338, ABCP, BCRP, BCRP1, MXR, ABCG2, Broad substrate specificity ATP-binding cassette transporter ABCG2, ATP-binding cassette sub-family G member 2, Breast cancer resistance protein, CDw338, Mitoxantrone resistance-associated protein, Placenta-specific ATP-binding cassette transporter, Urate exporter

6 Images

Immunocytochemistry/ Immunofluorescence - Anti-BCRP/ABCG2 antibody [EPR21122] (AB229193)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BCRP/ABCG2 antibody [EPR21122] (AB229193)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized JAR (human placenta choriocarcinoma cell line) cells labeling BCRP/ABCG2 with ab229193 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in JAR cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-BCRP/ABCG2 antibody [EPR21122] (AB229193)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A549 (human lung carcinoma cell line) cells labeling BCRP/ABCG2 with ab229193 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in A549 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR21122] (AB229193)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR21122] (AB229193)

Immunohistochemical analysis of paraffin-embedded human esophagus cancer tissue labeling BCRP/ABCG2 with ab229193 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP) . Membrane and cytoplasmic staining in esophageal cancer (PMID : 17671398) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR21122] (AB229193)

Immunohistochemical analysis of paraffin-embedded human brain tissue labeling BCRP/ABCG2 with ab229193 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in endothelial cells of human brain (PMID : 28912477) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Flow Cytometry - Anti-BCRP/ABCG2 antibody [EPR21122] (AB229193)

Flow Cyt

Unknown

Flow Cytometry - Anti-BCRP/ABCG2 antibody [EPR21122] (AB229193)

Flow cytometric analysis of A549 (human lung carcinoma cell line) cell line labeling BCRP/ABCG2 with ab229193 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on total viable cells.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCRP/ABCG2 antibody [EPR21122] (AB229193)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling BCRP/ABCG2 with ab229193 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic staining in human placenta (PMID : 28623970) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

Perform heat-mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

不同偶联物与剂型 (1)

Carrier free

Anti-BCRP/ABCG2 antibody [EPR21122] - BSA and Azide free

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

EPR21122

亚型

IgG

不含载体蛋白

反应种属

Human

应用

Flow Cyt, IHC-P, ICC/IF

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/60", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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产品详情

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

运输条件

Conditional Ambient

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Broad substrate specificity ATP-dependent transporter of the ATP-binding cassette (ABC) family that actively extrudes a wide variety of physiological compounds, dietary toxins and xenobiotics from cells (PubMed : 11306452, PubMed : 12958161, PubMed : 19506252, PubMed : 20705604, PubMed : 28554189, PubMed : 30405239, PubMed : 31003562). Involved in porphyrin homeostasis, mediating the export of protoporphyrin IX (PPIX) from both mitochondria to cytosol and cytosol to extracellular space, it also functions in the cellular export of heme (PubMed : 20705604, PubMed : 23189181). Also mediates the efflux of sphingosine-1-P from cells (PubMed : 20110355). Acts as a urate exporter functioning in both renal and extrarenal urate excretion (PubMed : 19506252, PubMed : 20368174, PubMed : 22132962, PubMed : 31003562, PubMed : 36749388). In kidney, it also functions as a physiological exporter of the uremic toxin indoxyl sulfate (By similarity). Also involved in the excretion of steroids like estrone 3-sulfate/E1S, 3beta-sulfooxy-androst-5-en-17-one/DHEAS, and other sulfate conjugates (PubMed : 12682043, PubMed : 28554189, PubMed : 30405239). Mediates the secretion of the vitamins riboflavin and biotin into milk (By similarity). Involved in the excretion of the riboflavin-derived compound lumichrome into the intestinal lumen and in its secretion into milk (PubMed : 39337371). Extrudes pheophorbide a, a phototoxic porphyrin catabolite of chlorophyll, reducing its bioavailability (By similarity). Plays an important role in the exclusion of xenobiotics from the brain (Probable). It confers to cells a resistance to multiple drugs and other xenobiotics including mitoxantrone, pheophorbide, camptothecin, methotrexate, azidothymidine, and the anthracyclines daunorubicin and doxorubicin, through the control of their efflux (PubMed : 11306452, PubMed : 12477054, PubMed : 15670731, PubMed : 18056989, PubMed : 31254042). In placenta, it limits the penetration of drugs from the maternal plasma into the fetus (By similarity). May play a role in early stem cell self-renewal by blocking differentiation (By similarity). In inflammatory macrophages, exports itaconate from the cytosol to the extracellular compartment and limits the activation of TFEB-dependent lysosome biogenesis involved in antibacterial innate immune response.

See full target information ABCG2

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文献 (8)

Recent publications for all applications. Explore the full list and refine your search

Journal, genetic engineering & biotechnology 23:100496 PubMed40390503

2025

Co-expression of HIF1A with multi-drug transporters (P-GP, MRP1, and BCRP) in chemoresistant breast, colorectal, and ovarian cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Sudipta Deb Nath,Md Tamzid Hossain Tanim,Md Mahmudul Hasan Akash,Mohammad Golam Mostafa,Abu Ashfaqur Sajib

International journal of molecular medicine 53: PubMed38426604

2024

Adipocyte‑rich microenvironment promotes chemoresistance via upregulation of peroxisome proliferator‑activated receptor gamma/ABCG2 in epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Siqi Chen,Zixuan Liu,Haixia Wu,Bo Wang,Yuqing Ouyang,Junru Liu,Xiaoyan Zheng,Haoke Zhang,Xueying Li,Xiaofan Feng,Yan Li,Yangyang Shen,Hong Zhang,Bo Xiao,Chunyan Yu,Weimin Deng

Cell proliferation 57:e13611 PubMed38356373

2024

iPSC-derived cells stimulate ABCG2/NES endogenous trabecular meshwork cell proliferation and tissue regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Gaiping Xi,Pengchao Feng,Xiaoyan Zhang,Shen Wu,Jingxue Zhang,Xiangji Wang,Ailing Xiang,Wenhua Xu,Ningli Wang,Wei Zhu

Experimental and therapeutic medicine 26:389 PubMed37456160

2023

E2F1‑mediated RAB34 upregulation accelerates the proliferation and inhibits the cell cycle arrest and apoptosis of acute myeloid leukemia cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojie Xiong,Gang Jian

Stem cell research & therapy 14:89 PubMed37061739

2023

Expansion and characterization of human limbus-derived stromal/mesenchymal stem cells in xeno-free medium for therapeutic applications.

Applications

Unspecified application

Species

Unspecified reactive species

Abhishek Sahoo,Mukesh Damala,Jilu Jaffet,Deeksha Prasad,Sayan Basu,Vivek Singh

Cancers 15: PubMed36612300

2023

Protoporphyrin IX (PpIX) Fluorescence during Meningioma Surgery: Correlations with Histological Findings and Expression of Heme Pathway Molecules.

Applications

Unspecified application

Species

Unspecified reactive species

Dorothee C Spille,Eva C Bunk,Christian Thomas,Zeynep Özdemir,Andrea Wagner,Burak H Akkurt,Manoj Mannil,Werner Paulus,Oliver M Grauer,Walter Stummer,Volker Senner,Benjamin Brokinkel

Clinical and translational medicine 12:e876 PubMed35605028

2022

APE1 shRNA-loaded cancer stem cell-derived extracellular vesicles reverse Erlotinib resistance in non-small cell lung cancer via the IL-6/STAT3 signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Chun-Han Tang,Ling Qin,Ying-Chun Gao,Tai-Yu Chen,Ke Xu,Tao Liu,Tao Ren

Biotechnology and bioengineering 117:748-762 PubMed31709508

2019

3D brain angiogenesis model to reconstitute functional human blood-brain barrier in vitro.

Applications

Unspecified application

Species

Unspecified reactive species

Somin Lee,Minhwan Chung,Seung-Ryeol Lee,Noo Li Jeon

View all publications

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Anti-BCRP/ABCG2 抗体 [EPR21122]