重组Anti-Bcl6抗体[RM1183] (ab317337)
![Western blot - Anti-Bcl6 antibody [RM1183] (ab317337)](https://www.abcam.cn/ps/products/317/ab317337/Images/ab317337-539787-anti-bcl6-antibody-rm1183-western-blot-ramos-human-burkitts-lymphoma-b-lymphocyte-whole-cell-lysate-20-ug.jpg "Western blot - Anti-Bcl6 antibody [RM1183] (ab317337)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit recombinant multiclonal [RM1183] to Bcl6
- Suitable for: WB, IHC-P, IP
- Reacts with: Human
Related conjugates and formulations
概述
-
产品名称
Anti-Bcl6抗体[RM1183]
参阅全部 Bcl6 一抗 -
描述
兔重组multiclonal [RM1183] to Bcl6 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, IPmore details
不适用于: Flow Cyt (Intra) or ICC/IF -
种属反应性
与反应: Human
不与反应: Mouse, Rat -
免疫原
This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers. -
阳性对照
- WB: Ramos, Ramos whole, Daudi and Raji lysates. IHC-P: Human tonsil, Human diffuse large B- lymphoma and Human non-hodgkin's lymphoma tissues IP: Ramos cell.
-
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
Recombinant Multiclonal -
克隆编号
RM1183 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Related Products
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Anti-Bcl6 antibody [EPR11410-43] (ab172610)
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
- Anti-Bcl6 antibody [SP155] - C-terminal (ab183308)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab317337于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000.
|
|
| IHC-P |
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
|
|
| IP |
1/30.
|
| 说明 |
|---|
|
WB
1/1000. |
|
IHC-P
1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
|
IP
1/30. |
靶标
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功能
Transcriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis. -
组织特异性
Expressed in germinal center T and B cells and in primary immature dendritic cells. -
疾病相关
Note=Chromosomal aberrations involving BCL6 may be a cause of B-cell non-Hodgkin lymphoma. Translocation t(3;14)(q27;q32); translocation t(3;22)(q27;q11) with immunoglobulin gene regions.
Note=A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1.
Note=A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF. -
序列相似性
Contains 1 BTB (POZ) domain.
Contains 6 C2H2-type zinc fingers. -
结构域
The BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2. -
翻译后修饰
Phosphorylated by MAPK1 in response to antigen receptor activation. Phosphorylation induces its degradation by ubiquitin/proteasome pathway. -
细胞定位
Nucleus. - Information by UniProt
-
数据库链接
- Entrez Gene: 604 Human
- Omim: 109565 Human
- SwissProt: P41182 Human
- Unigene: 478588 Human
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别名
- B cell CLL/lymphoma 6 antibody
- B cell lymphoma 6 protein antibody
- B-cell lymphoma 5 protein antibody
see all
图片
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Western blot - Anti-Bcl6 antibody [RM1183] (ab317337)All lanes : Anti-Bcl6 antibody [RM1183] (ab317337) at 1/1000 dilution
Lane 1 : Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate
Lane 3 : Ramos whole cell lysate
Lane 4 : Jurkat whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 78 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: Jurkat (PMID: 8652841)
This antibody was tested on fresh and frozen Romas and Jurkat Whole Cell Lysate.
The Lysates in lanes 3-4 were freshly made and used for Western Blotting immediately to minimize protein degradation.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
-
![Western blot - Anti-Bcl6 antibody [RM1183] (ab317337)](https://www.abcam.cn/ps/products/317/ab317337/Images/ab317337-539788-anti-bcl6-antibody-rm1183-western-blot-daudi-human-burkitts-lymphoma-lymphoblast-whole-cell-lysate-20-ug.jpg)
Western blot - Anti-Bcl6 antibody [RM1183] (ab317337)All lanes : Anti-Bcl6 antibody [RM1183] (ab317337) at 1/1000 dilution
Lane 1 : Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate
Lane 2 : Raji (human Burkitts lymphoma B lymphocyte) whole cell lysate
Lane 3 : 293T (human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Observed band size: 78 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking and diluting buffer and concentration: 5% NFDM/TBST.
Low expression: 293T
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337)](https://www.abcam.cn/ps/products/317/ab317337/Images/ab317337-1-anti-bcl6-antibody-rm1183-immunohistochemistry-human-tonsil.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling Bcl6 with ab317337 at 1/1000 (0.495 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human tonsil. The section was incubated with ab317337 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337)](https://www.abcam.cn/ps/products/317/ab317337/Images/ab317337-2-anti-bcl6-antibody-rm1183-immunohistochemistry-human-diffuse-large.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337)Immunohistochemical analysis of paraffin-embedded Human diffuse large B-cell lymphoma tissue labeling Bcl6 with ab317337 at 1/1000 (0.495 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human diffuse large B-cell lymphoma. The section was incubated with ab317337 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND� RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337)](https://www.abcam.cn/ps/products/317/ab317337/Images/ab317337-3-anti-bcl6-antibody-rm1183-immunohistochemistry-human-non-hodgkins.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337)Immunohistochemical analysis of paraffin-embedded Human non-hodgkin's lymphoma tissue labeling Bcl6 with ab317337 at 1/1000 (0.495 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Positive staining on human non-hodgkin's lymphoma. The section was incubated with ab317337 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337)](https://www.abcam.cn/ps/products/317/ab317337/Images/ab317337-4-anti-bcl6-antibody-rm1183-immunohistochemistry-human-cerebrum.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337)Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Bcl6 with ab317337 at 1/1000 (0.495 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human cerebrum. The section was incubated with ab317337 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337)](https://www.abcam.cn/ps/products/317/ab317337/Images/ab317337-5-anti-bcl6-antibody-rm1183-immunohistochemistry-human-liver.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [RM1183] (ab317337)Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Bcl6 with ab317337 at 1/1000 (0.495 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Negative control: no staining on human liver. The section was incubated with ab317337 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrumentCounterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).
Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
-
![Immunoprecipitation - Anti-Bcl6 antibody [RM1183] (ab317337)](https://www.abcam.cn/ps/products/317/ab317337/Images/ab317337-6-anti-bcl6-antibody-rm1183-immunoprecipitation-ramos.jpg)
Immunoprecipitation - Anti-Bcl6 antibody [RM1183] (ab317337)Bcl6 was immunoprecipitated from 0.35 mg Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate with ab317337 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab317337 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 2: ab317337 IP in Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab317337 in Ramos whole cell lysateBlocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.
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![Anti-Bcl6 antibody [RM1183] (ab317337)](https://www.abcam.cn/ps/products/317/ab317337/Images/ab317337-7-benefitsofrecombinantantibodies.jpg)
Anti-Bcl6 antibody [RM1183] (ab317337)
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab317337 尚未被引用在任何文献中。