重组Anti-Bcl6抗体[EPR11410-43] (ab172610)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)](https://www.abcam.cn/ps/products/172/ab172610/Images/ab172610-307086-anti-bcl-6-antibody-epr11410-43-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11410-43] to Bcl6
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Bcl6抗体[EPR11410-43]
参阅全部 Bcl6 一抗 -
描述
兔单克隆抗体[EPR11410-43] to Bcl6 -
宿主
Rabbit -
特异性
We tested the antibody in mouse and rat samples and tests were unsuccessful therefore we can no longer guaranteed the reactivity of this species.
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经测试应用
适用于: WB, IHC-Pmore details
不适用于: Flow Cyt,ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Recombinant fragment within Human bcl-6 aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: P41182 -
阳性对照
- Ramos, Daudi and Raji cell lysates; Human lymph node, tonsil and B cell lymphoma tissues; Ramos cells.
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常规说明
We tested the antibody in mouse and rat samples and tests were unsuccessful therefore we can no longer guaranteed the reactivity of this species.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR11410-43 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab172610于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 87-98 kDa (predicted molecular weight: 78 kDa).
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| IHC-P | (3) |
1/1500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
For unpurified use at 1/200 - 1/500. See IHC antigen retrieval protocols. |
| 说明 |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 87-98 kDa (predicted molecular weight: 78 kDa). |
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IHC-P
1/1500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. For unpurified use at 1/200 - 1/500. See IHC antigen retrieval protocols. |
靶标
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功能
Transcriptional repressor which is required for germinal center formation and antibody affinity maturation. Probably plays an important role in lymphomagenesis. -
组织特异性
Expressed in germinal center T and B cells and in primary immature dendritic cells. -
疾病相关
Note=Chromosomal aberrations involving BCL6 may be a cause of B-cell non-Hodgkin lymphoma. Translocation t(3;14)(q27;q32); translocation t(3;22)(q27;q11) with immunoglobulin gene regions.
Note=A chromosomal aberration involving BCL6 may be a cause of a form of B-cell leukemia. Translocation t(3;11)(q27;q23) with POU2AF1/OBF1.
Note=A chromosomal aberration involving BCL6 may be a cause of lymphoma. Translocation t(3;4)(q27;p11) with ARHH/TTF. -
序列相似性
Contains 1 BTB (POZ) domain.
Contains 6 C2H2-type zinc fingers. -
结构域
The BTB domain mediates homodimerization. Its dimer interface mediates peptide binding such as to corepressors BCOR and NCOR2. -
翻译后修饰
Phosphorylated by MAPK1 in response to antigen receptor activation. Phosphorylation induces its degradation by ubiquitin/proteasome pathway. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 604 Human
- Omim: 109565 Human
- SwissProt: P41182 Human
- Unigene: 478588 Human
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别名
- B cell CLL/lymphoma 6 antibody
- B cell lymphoma 6 protein antibody
- B-cell lymphoma 5 protein antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymph node in stomach tissue sections labeling bcl-6 with purified ab172610 at 1:1500 dilution (0.5 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
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![Western blot - Anti-Bcl6 antibody [EPR11410-43] (ab172610)](https://www.abcam.cn/ps/products/172/ab172610/Images/ab172610-307070-anti-bcl-6-antibody-epr11410-43-western-blot.jpg)
Western blot - Anti-Bcl6 antibody [EPR11410-43] (ab172610)Anti-Bcl6 antibody [EPR11410-43] (ab172610) at 1/2000 dilution (Purified) + Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 20000 µg
Predicted band size: 78 kDa
Observed band size: 78 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)](https://www.abcam.cn/ps/products/172/ab172610/Images/ab172610-195852-anti-bcl-6-antibody-epr11410-43-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.
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![Western blot - Anti-Bcl6 antibody [EPR11410-43] (ab172610)](https://www.abcam.cn/ps/products/172/ab172610/Images/ab172610-195851-anti-bcl-6-antibody-epr11410-43-western-blot.jpg)
Western blot - Anti-Bcl6 antibody [EPR11410-43] (ab172610)All lanes : Anti-Bcl6 antibody [EPR11410-43] (ab172610) at 1/1000 dilution
Lane 1 : Ramos cell lysate
Lane 2 : Daudi cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 78 kDaThis image was produced using unpurified antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)](https://www.abcam.cn/ps/products/172/ab172610/Images/ab172610-195853-anti-bcl-6-antibody-epr11410-43-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)](https://www.abcam.cn/ps/products/172/ab172610/Images/ab172610-301443-anti-bcl-6-antibody-epr11410-43-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)This image is courtesy of an anonymous Abreviewab172610 staining bcl-6 in Human tonsil tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with formaldehyde, blocked with 1% PB ab64226 for 10 minutes at room tempreture and antigen retrieval was by heat mediation in EDTA pH 9. The sample was incubated with primary antibody (1/100 in Leica Bond Diluent) at for 30 minutes. A HRP-conjugated Goat anti-rabbit polyclonal (undiluted) was used as the secondary antibody. This image was produced using unpurified antibody.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)](https://www.abcam.cn/ps/products/172/ab172610/Images/ab172610-195854-anti-bcl-6-antibody-epr11410-43-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl6 antibody [EPR11410-43] (ab172610)Immunohistochemical analysis of paraffin-embedded Human B cell lymphoma tissue labeling bcl-6 with ab172610 at 1/250 dilution. This image was produced using unpurified antibody.
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![Anti-Bcl6 antibody [EPR11410-43] (ab172610)](https://www.abcam.cn/ps/products/172/ab172610/Images/ab172610-9-benefits-of-recombinant-antibodies.png)
Anti-Bcl6 antibody [EPR11410-43] (ab172610)
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab172610 被引用在 10 文献中.
- Lynch TJ et al. Ferret Lung Transplantation Models Differential Lymphoid Aggregate Morphology Between Restrictive and Obstructive Forms of Chronic Lung Allograft Dysfunction. Transplantation 106:1974-1989 (2022). PubMed: 35442232
- Lin JM et al. BCL-6 promotes the methylation of miR-34a by recruiting EZH2 and upregulating CTRP9 to protect ischemic myocardial injury. Biofactors 47:386-402 (2021). PubMed: 33502806
- Suzuki M et al. Utility of a Reverse Phase Protein Array to Evaluate Multiple Biomarkers in Diffuse Large B-Cell Lymphoma. Proteomics Clin Appl 14:e1900091 (2020). PubMed: 31721454
- Khaleefah MM et al. Isolated oculomotor nerve palsy as a manifestation of diffuse large B cell lymphoma: A case report. Oncol Lett 20:285 (2020). PubMed: 33014163
- Li N et al. Pyruvate Kinase M2 Knockdown Suppresses Migration, Invasion, and Epithelial-Mesenchymal Transition of Gastric Carcinoma via Hypoxia-Inducible Factor Alpha/B-Cell Lymphoma 6 Pathway. Biomed Res Int 2020:7467104 (2020). PubMed: 33376737
- Dheilly E et al. Cathepsin S Regulates Antigen Processing and T Cell Activity in Non-Hodgkin Lymphoma. Cancer Cell 37:674-689.e12 (2020). PubMed: 32330455
- Wang K et al. MicroRNA-155 promotes neointimal hyperplasia through smooth muscle-like cell-derived RANTES in arteriovenous fistulas. J Vasc Surg 67:933-944.e3 (2018). PubMed: 29477204
- Song Y et al. Increased frequency of thymic T follicular helper cells in myasthenia gravis patients with thymoma. J Thorac Dis 8:314-22 (2016). IHC ; Human . PubMed: 27076925
- Sun Y et al. MicroRNA-155 Promotes the Directional Migration of Resident Smooth Muscle Progenitor Cells by Regulating Monocyte Chemoattractant Protein 1 in Transplant Arteriosclerosis. Arterioscler Thromb Vasc Biol 36:1230-9 (2016). PubMed: 27079875
- Meyerholz DK et al. Immunohistochemical Detection of Markers for Translational Studies of Lung Disease in Pigs and Humans. Toxicol Pathol 44:434-41 (2016). IHC-P ; Pig . PubMed: 26511846