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Anti-Bcl3抗体(ab27780)

Reviews (2)Q&A (6)References (2)
Western blot - Anti-Bcl3 antibody (ab27780)

    Key features and details

    • Rabbit polyclonal to Bcl3
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-Bcl3抗体
      参阅全部 Bcl3 一抗

    • 描述

      兔多克隆抗体to Bcl3

    • 宿主

      Rabbit

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human

    • 免疫原

      Synthetic peptide corresponding to Human Bcl3. Synthetic peptide surrounding amino acid 424 of human Bcl-3
      Database link: P20749

    • 阳性对照

      • WB: Jurkat

    • 常规说明

      This product is manufactured by BioVision, an Abcam company and was previously called 3407 Bcl-3 Antibody. 3407-100 is the same size as the 100 µg size of ab27780.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab27780于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB (1)
    Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 47 kDa).
    说明
    WB
    Use a concentration of 1 - 4 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 47 kDa).

    靶标

    • 功能

      Contributes to the regulation of transcriptional activation of NF-kappa-B target genes. In the cytoplasm, inhibits the nuclear translocation of the NF-kappa-B p50 subunit. In the nucleus, acts as transcriptional activator that promotes transcription of NF-kappa-B target genes. Contributes to the regulation of cell proliferation.

    • 疾病相关

      Note=A chromosomal aberration involving BCL3 may be a cause of B-cell chronic lymphocytic leukemia (B-CLL). Translocation t(14;19)(q32;q13.1) with immunoglobulin gene regions.

    • 序列相似性

      Contains 7 ANK repeats.

    • 翻译后修饰

      Polyubiquitinated. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. Deubiquitination by CYLD prevents nuclear accumulation.
      Activated by phosphorylation.

    • 细胞定位

      Nucleus. Cytoplasm. Cytoplasm > perinuclear region. Ubiquitination via 'Lys-63'-linked ubiquitin chains is required for nuclear accumulation.
    • Information by UniProt

    • 数据库链接

      • 别名

        • B cell CLL/lymphoma 3 antibody
        • B cell leukemia/lymphoma 3 antibody
        • B cell lymphoma 3 encoded protein antibody
        • B-cell lymphoma 3 protein antibody
        • Bcl 3 antibody
        • BCL 4 antibody
        • BCL-3 antibody
        • Bcl3 antibody
        • BCL3_HUMAN antibody
        • BCL4 antibody
        • Chronic lymphatic leukemia protein antibody
        • D19S37 antibody
        • Protein Bcl 3 antibody
        • Proto-oncogene BCL3 antibody
        see all

      图片

      • Western blot - Anti-Bcl3 antibody (ab27780)
        Western blot - Anti-Bcl3 antibody (ab27780)
        Anti-Bcl3 antibody (ab27780) at 2 µg/ml + Jurkat

        Predicted band size: 47 kDa

      文献 (2)

      发表研究结果有使用 ab27780？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab27780 被引用在 2 文献中.

      • Shao M  et al. MYBL2 protects against H9c2 injury induced by hypoxia via AKT and NF-?B pathways. Mol Med Rep 17:4832-4838 (2018). PubMed: 29328450
      • Long TY  et al. CIRBP protects H9C2 cells against myocardial ischemia through inhibition of NF-?B pathway. Braz J Med Biol Res 50:e5861 (2017). PubMed: 28355355

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      1-8 of 8 Abreviews or Q&A

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Bcl3 antibody

       Inconclusive
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (pigmented skin lessions)
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: EnVision Flex hight Dako
      Permeabilization
      No
      Specification
      pigmented skin lessions
      Blocking step
      (agent) for 1 hour(s) and 0 minute(s) · Concentration: 10µg/mL · Temperature: 37°C
      Fixative
      Formaldehyde
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Mar 22 2017

      Western blot abreview for Anti-Bcl3 antibody

       Poor
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Colon)
      Loading amount
      80 µg
      Specification
      Colon
      Treatment
      Basal and Knockdown (siRNA)
      Gel Running Conditions
      Reduced Denaturing (10%)
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      Abcam user community

      Verified customer

      提交于 Nov 23 2012

      Question

      Dear xxxx
      Thank you for sending the two antibodies, I tried them both using our
      standard method (works with 99% of antibodies we use). The Bcl-3 rabbit
      (ab13708) was very disappointing, there may be a slight decrease in a band
      with knockdown, but basal levels between cell-line appear identical.
      On a good note, the mouse (ab49470) looks really promising this is showing
      similar basal levels in cell-lines to previous findings and a clear
      reduction in band with siRNA. I would like to rerun some samples on a low
      percentage gel, as there is a non-specific band running just below it. I
      would also like to check the antibody detects our overexpression plasmids.
      In completion of my findings I will do the required abreview.
      I'm quite excited that we may have found a 'working' Bcl-3 antibody.
      With regards

      Read More
      Answer

      Thank you very much for that feedback.

      I am pleased to hear that the anti-Bcl3 antibody ab49470 is showing promise in your experiments. This information would be very valuable to other customers so I would be very interested to hear when you have submitted the Abreview with your findings.

      Equally, it is disappointing that the ab13708 did not work very well for you. If you would not mind submitting a review with these results, it also allows customers to evaluate the antibody in light of your results. This should only take 5-10 minutes and you will be rewarded with Abpoints which can be used to claim Amazon vouchers or credit for future orders from Abcam.

      If you could let me know once you have submitted your reviews that would be great as then I can be the one to review and publish them. As I know the background to this case I can make sure they are published to the website in the correct way.

      If I can be of any further assistance, please do let me know. Until then, I wish you all the best with your research.

      Read More

      Question


      I would be more than happy to try out the mouse monoclonal, I have a
      freezer full of Bcl-3 knockdown western samples and matching RNA preps.
      This would be a really good test for the antibody.
      Just to let you know, I will be on holiday for two weeks after this week,
      so expect a slight delay.
      Regards

      Read More
      Answer

      That is no problem at all, we would be happy to receive the feedback whenever you are ready to give it.

      I have now arranged for the two antibodies we discussed to be sent to you (free of charge). The antibody ab13708 is however not directly in stock and I have an estimated date of delivery onFriday the21st of September. As you are going away, would you prefer for me to delay the delivery until you are back? If so, which date would be most convenient for you.

      I look forward to receiving your reply.

      Read More

      Question


      Thank you for getting back to me, yes I agree BSA could help but the
      antibody isn't even picking up the correct band - the siRNA pairing
      emphasizes this clearly. I sent you a low exposure film as the antibody
      brought up numerous non specific bands.
      Could I try a different antibody, either ab15046 or ab13708, which ever is
      your best seller?
      With kind regards

      Read More
      Answer

      Thank you for getting back to me.

      I can provide you any antibody which you would prefer. Ab13708 is marginally more popular but not by much. I will also check if we have any information on if the mouse monoclonal ab49470 is likely to work in western blotting as this has been quite popular for using in IHC.

      I look forward toreceiving your reply.

      Read More

      Question

      1) Abcam product code ab27780

      Read More
      Answer

      Sorry for the delay in getting back to you.

      I have checked the QC data for the particular lot of ab27780 you have been using and it looks as clean as the Western blot presented on the datasheet of this product using Jurkat cell lysate. One thing that may be worth trying is that we use BSA in blocking, not milk as you have used (this can sometimes make a remarkable difference in the results observed, as presented on the datasheet of ab9385).

      However, I understand that you have spent considerable time in trying to optimise this experiment and I can arrange for a replacement antibody to be sent to you if you would like? We do not unfortunately have an alternative lot to the one you tried of ab27780 but I could arrange for an alternative antibody altogether. I can even send you the antibody ab49470 although we have not as yet tested this in Western blotting.

      Alternatively, if you would prefer, I can arrange for a credit note or refund to be raised.

      I look forward to receiving your reply.

      Read More

      Question

      Phone call reporting problems in using the Anti-Bcl3 antibody (ab27780) in western blotting.

      Read More
      Answer

      Thank you for calling us yesterday and for alerting us to the problem you are experiencing with theAnti-Bcl3 antibody (ab27780) and sorry for the delay in getting back to you. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly.

      As we discussed on the phone, I haveattached our questionnaire so that we can gather further information regarding the samples tested and the protocol used. OnceI have receive the completed questionnaire,I will look at the protocol and see if there are any suggestions we can make that may improve the results.

      This information will also allow us to investigate this case internally and initiate additional testing where necessary. If the product was purchased in the last six months and is being used according to our Abpromise, we would be happy to replace or refund the antibody.

      I look forward to receiving your reply.

      Read More

      Question

      Dear
      Just to let you know, I have this morning uploaded three reviews onto your
      web site, for the three BCL-3 antibodies you have sent me over the last few
      months.
      Ab27780 and ab13708 are rather damming, I really don't think they are
      picking up the correct band, possibly ab13708, but I think it unlikely. We
      had been working on BCL-3 for about three years before the Santa Cruz
      antibody we were using stopped performing, but up to that point all our
      work was consistent, with basal levels and various knockdown and
      overexpression experiments. We have QPCR data to back up our western
      findings.
      The mouse ab49470 is giving identical results to those we had been
      originally seeing. I am convinced this is BCL-3, the antibody is detecting
      at 55kDa. Not sure if the lower band at 50kDa is non-specific or a related
      unphospho-BCL-3 band, need further investigation.
      Thank you again for allowing me to test the 'successful' antibody - funnily
      enough we did have an odd vial of it in the lab for staining - but your
      data sheet clearly stated not for westerns. hence we didn't even consider
      it.
      We now need to consider purchasing this in bulk, possibly 5 vials -
      GR54873, so things do not go belly-up again. Could you arrange this for me?
      Get back to me if there is a problem with the figure, sorry I know I should
      have numbered the lanes, but I lifted them straight for a presentation - on
      the traumas of antibodies!!
      With Kind Regards

      Read More
      Answer

      Thank you for your email.

      I am pleased to hear that the ab49470 worked for you. I am currently seeking information as to whether we have a further 5 vials of the lot you tried in stock. I will get back to you with this information as soon as I have it.

      I am pleased you submitted all the Abreviews. I will have a look through them by the end of the week and will let you know if there is any further information needed. I think this will be very valuable information for our customers. Thank you very much for doing this.

      Read More

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