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重组RabMAb

重组Anti-Bcl-XL抗体[EPR16642] - BSA and Azide free (ab223547)

Submit a review Submit a question References (1)
Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
  • Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
  • Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
  • Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
  • Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
  • Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16642] to Bcl-XL - BSA and Azide free
  • Suitable for: IHC-P, IP, WB
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Unconjugated

概述

  • 产品名称

    Anti-Bcl-XL抗体[EPR16642] - BSA and Azide free
    参阅全部 Bcl-XL 一抗

  • 描述

    兔单克隆抗体[EPR16642] to Bcl-XL - BSA and Azide free

  • 宿主

    Rabbit

  • 经测试应用

    适用于: IHC-P, IP, WBmore details
    不适用于: Flow Cyt or ICC/IF

  • 种属反应性

    与反应: Mouse, Rat, Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: Ramos, Jurkat, HeLa or K562 whole cell lysate. Mouse brain, heart, kidney, spleen. Rat brain, heart, kidney, spleen. C6, RAW 264.7, PC-12 or NIH/3T3 whole cell lysate. IHC-P: Human kidney, mouse colon, rat testis.

  • 常规说明

    ab223547 is the carrier-free version of ab178844.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab223547于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
说明
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP
Use at an assay dependent concentration.
WB
Use at an assay dependent concentration. Detects a band of approximately 30 kDa (predicted molecular weight: 26 kDa).
应用说明
Is unsuitable for Flow Cyt or ICC/IF.

靶标

  • 功能

    Potent inhibitor of cell death. Inhibits activation of caspases (By similarity). Appears to regulate cell death by blocking the voltage-dependent anion channnel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane.
    Isoform Bcl-X(S) promotes apoptosis.

  • 组织特异性

    Bcl-X(S) is expressed at high levels in cells that undergo a high rate of turnover, such as developing lymphocytes. In contrast, Bcl-X(L) is found in tissues containing long-lived postmitotic cells, such as adult brain.

  • 序列相似性

    Belongs to the Bcl-2 family.

  • 结构域

    The BH4 motif is required for anti-apoptotic activity. The BH1 and BH2 motifs are required for both heterodimerization with other Bcl-2 family members and for repression of cell death.

  • 翻译后修饰

    Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity.

  • 细胞定位

    Mitochondrion membrane. Nucleus membrane. Mitochondrial membranes and perinuclear envelope.
  • Information by UniProt

  • 数据库链接

    see all

  • 别名

    • Apoptosis regulator Bcl X antibody
    • Apoptosis regulator Bcl-X antibody
    • Apoptosis regulator BclX antibody
    • B cell lymphoma 2 like antibody
    • B2CL1_HUMAN antibody
    • Bcl 2 like 1 protein antibody
    • Bcl X antibody
    • Bcl xL antibody
    • BCL XL/S antibody
    • Bcl xS antibody
    • Bcl-2-like protein 1 antibody
    • Bcl2 Like 1 antibody
    • Bcl2 related gene antibody
    • Bcl2-L-1 antibody
    • BCL2L antibody
    • Bcl2l1 antibody
    • BCLX antibody
    • BclXL antibody
    • BclXs antibody
    • DKFZp781P2092 antibody
    • PPP1R52 antibody
    • Protein phosphatase 1 regulatory subunit 52 antibody
    see all

图片

  • Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    All lanes : Anti-Bcl-XL antibody [EPR16642] (ab178844) at 1/20000 dilution

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 4 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa



    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)

    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with ab178844 at 1:2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Cytoplasmic staining on epithelial cells of tubules and glomeruli of kidney is observed. Negative control: Using PBS instead of primary ab, secondary ab as above. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)

    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Cytoplasm staining on epithelial cells of mouse colon tissue is observed. Negative control: Using PBS instead of primary ab, secondary ab as above. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)

    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Rat tesits labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Cytoplasm staining on epithelial cells of testis is observed. Negative control: Using PBS instead of primary ab, secondary ab as above. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)

    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with ab178844 at 1/70 dilution. Western blot was performed of the immunoprecipitate using ab178844 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.Blocking buffer and concentration: 5% NFDM/TBST. Diluting buffer and concentration:5% NFDM/TBST.
  • Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    All lanes : Anti-Bcl-XL/S [EPR16642] antibody (ab178844) at 1/20000 dilution, and a competitor's rabbit polyclonal antibody at 1/100 dilution

    Lane 1 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
    Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
    Lane 3 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 4 : K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate

    Secondary
    All lanes : Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution

    Developed using the ECL technique.

    Predicted band size: 26 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?



    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    This western blot image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

  • Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Western blot - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    All lanes : Anti-Bcl-XL/S [EPR16642] antibody (ab178844) at 1/20000 dilution, and a competitor's rabbit polyclonal antibody at 1/100 dilution

    Lane 1 : Mouse brain
    Lane 2 : Mouse heart
    Lane 3 : Mouse kidney
    Lane 4 : Mouse spleen
    Lane 7 : Rat kidney
    Lane 8 : Rat spleen
    Lane 9 : C6 whole cell lysates
    Lane 10 : Raw264.7 whole cell lysates
    Lane 11 : PC-12 whole cell lysates
    Lane 12 : NIH/3T3 whole cell lysates

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

    Predicted band size: 26 kDa
    Observed band size: 30 kDa why is the actual band size different from the predicted?



    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    This western blot image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)

    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    This IHC image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody in which both antibodies were tested on Human kidney tissue. Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with ab178844 at 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
    Cytoplasmic staining on epithelial cells of tubules and glomeruli of kidney is observed.
    Negative control: Using PBS instead of primary ab, secondary ab as above.
    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with competitor rabbit polyclonal at 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    This data was developed using ab178844, the same antibody clone in a different buffer formulation.This IHC image is a comparison between ab178844 and competitor's leading rabbit polyclonal in which both antibodies were tested on Mouse colon tissue. Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
    Cytoplasm staining on epithelial cells of mouse colon tissue is observed.
    Negative control: Using PBS instead of primary ab, secondary ab as above.
    Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with competitor rabbit polyclonal antibody at a 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    This data was developed using ab178844, the same antibody clone in a different buffer formulation.This IHC image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody in which both antibodies were tested on Rat testis tissue.
    Immunohistochemical analysis of paraffin-embedded Rat tesits labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
    Cytoplasm staining on epithelial cells of testis is observed.
    Negative control: Using PBS instead of primary ab, secondary ab as above.
    Immunohistochemical analysis of paraffin-embedded Rat tesits labeling Bcl-XL with competitor rabbit polyclonal antibody at a 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)

    This data was developed using ab178844, the same antibody clone in a different buffer formulation.

    This immunoprecipitation image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

    Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with ab178844 at 1/70 dilution. Western blot was performed of the immunoprecipitate using ab178844 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.

    Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with competitor rabbit polyclonal antibody at 1/12 dilution. Western blot was performed of the immunoprecipitate using competitor antibody at 1/100 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane: Ramos whole cell extract. Right lane: PBS instead of Ramos whole cell extract.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)
    Anti-Bcl-XL antibody [EPR16642] - BSA and Azide free (ab223547)

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

文献 (1)

发表研究结果有使用 ab223547？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab223547 被引用在 1 文献中.

  • Li W  et al. PEX1 is a mediator of α1-adrenergic signaling attenuating doxorubicin-induced cardiotoxicity. J Biochem Mol Toxicol 36:e23196 (2022). PubMed: 35979984

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