重组Anti-Bcl-XL抗体[EPR16642]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (AB178844)

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with ab178844 at 1 : 2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

Cytoplasmic staining on epithelial cells of tubules and glomeruli of kidney is observed.

Negative control : Using PBS instead of primary ab secondary ab as above.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (AB178844)

This IHC image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody in which both antibodies were tested on Human kidney tissue.

Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with ab178844 at 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
Cytoplasmic staining on epithelial cells of tubules and glomeruli of kidney is observed.
Negative control : Using PBS instead of primary ab secondary ab as above.
Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Bcl-XL with competitor rabbit polyclonal at 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IP

Supplier Data

Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] (AB178844)

Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with ab178844 at 1/70 dilution. Western blot was performed of the immunoprecipitate using ab178844 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane : Ramos whole cell extract. Right lane : PBS instead of Ramos whole cell extract.

Blocking buffer and concentration : 5% NFDM/TBST. Diluting buffer and concentration :

5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] (ab178844)

Predicted band size: 26 kDa

false

• IP

Lab

Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] (AB178844)

This immunoprecipitation image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with ab178844 at 1/70 dilution. Western blot was performed of the immunoprecipitate using ab178844 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane : Ramos whole cell extract. Right lane : PBS instead of Ramos whole cell extract.
Bcl-XL/S was immunoprecipitated from 1mg of Ramos (Human Burkitt's lymphoma cell line) whole cell extract with competitor rabbit polyclonal antibody at 1/12 dilution. Western blot was performed of the immunoprecipitate using competitor antibody at 1/100 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution. Left lane : Ramos whole cell extract. Right lane : PBS instead of Ramos whole cell extract.
Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Bcl-XL antibody [EPR16642] (ab178844)

Predicted band size: 26 kDa

Observed band size: 30 kDa

false

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (AB178844)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

Cytoplasm staining on epithelial cells of mouse colon tissue is observed.

Negative control : Using PBS instead of primary ab secondary ab as above.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (AB178844)

Immunohistochemical analysis of paraffin-embedded Rat tesits labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

Cytoplasm staining on epithelial cells of testis is observed.

Negative control : Using PBS instead of primary ab secondary ab as above.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bcl-XL antibody [EPR16642] (AB178844)

This IHC image is a comparison between ab178844 and competitor's leading rabbit polyclonal in which both antibodies were tested on Mouse colon tissue.

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with ab178844 at a 1/2000 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.
Cytoplasm staining on epithelial cells of mouse colon tissue is observed.
Negative control : Using PBS instead of primary ab secondary ab as above.
Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Bcl-XL with competitor rabbit polyclonal antibody at a 1/100 dilution followed by prediluted HRP Polymer for Rabbit/Mouse IgG. Counter stained with hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• WB

Lab

Western blot - Anti-Bcl-XL antibody [EPR16642] (AB178844)

This western blot image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

Blocking buffer and concentration : 5% NFDM/TBST

All lanes:

Anti-Bcl-XL/S [EPR16642] antibody (ab178844) at 1/20000 dilution, and a competitor's rabbit polyclonal antibody at 1/100 dilution

Lane 1:

Ramos (Human Burkitt's lymphoma cell line) whole cell lysate

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate

Lane 4:

K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

Observed band size: 30 kDa

true

• WB

Supplier Data

Western blot - Anti-Bcl-XL antibody [EPR16642] (AB178844)

Blocking buffer and concentration : 5% NFDM/TBST

Diluting buffer and concentration : 5% NFDM /TBST

All lanes:

Western blot - Anti-Bcl-XL antibody [EPR16642] (ab178844) at 1/20000 dilution

Lane 1:

Ramos (Human Burkitt's lymphoma cell line) whole cell lysate at 20 µg

Lane 2:

Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg

Lane 3:

HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 4:

K562 (Human chronic myelogenous leukemia cells from bone marrow) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 26 kDa

true

• WB

Lab

Western blot - Anti-Bcl-XL antibody [EPR16642] (AB178844)

This western blot image is a comparison between ab178844 and a competitor's leading rabbit polyclonal antibody.

Blocking buffer and concentration : 5% NFDM/TBST

All lanes:

Anti-Bcl-XL/S [EPR16642] antibody (ab178844) at 1/20000 dilution, and a competitor's rabbit polyclonal antibody at 1/100 dilution

Lane 1:

Mouse brain

Lane 2:

Mouse heart

Lane 3:

Mouse kidney

Lane 4:

Mouse spleen

Lane 5:

Rat brain

Lane 6:

Rat heart

Lane 7:

Rat kidney

Lane 8:

Rat spleen

Lane 9:

C6 whole cell lysates

Lane 10:

Raw264.7 whole cell lysates

Lane 11:

PC-12 whole cell lysates

Lane 12:

NIH/3T3 whole cell lysates

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

Predicted band size: 26 kDa

Observed band size: 30 kDa

false

• WB

CiteAb

Western blot - Anti-Bcl-XL antibody [EPR16642] (AB178844)

Bcl-XL western blot using anti-Bcl-XL antibody [EPR16642] ab178844. Publication image and figure legend from Lian, S., Guo, J., et al., 2017, Oncotarget, PubMed 29383161.

ab178844 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab178844 please see the product overview.

Effect of prenatal cold stress on the expression of Bcl-xL, Bax and Caspase3 proteins(A) These are cropped blots, full-length blots are presented in Supplementary Figure 3. (B) Bcl-xL/Bax ratio was determined by western blot. (C) Caspase3 protein levels in placenta. The data are presented as mean ± SD (n = 5). Statistically significant differences are indicated : *p < 0.01, **p < 0.01.

false