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AB270253

Anti-Bcl-XL抗体[2H12]

Anti-Bcl-XL antibody [2H12]

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(3 Publications)

Mouse Monoclonal Bcl-XL antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human BCL2L1 aa 1-50.

查看别名

BCL2L, BCLX, BCL2L1, Bcl-2-like protein 1, Bcl2-L-1, Apoptosis regulator Bcl-X

1 Images
Western blot - Anti-Bcl-XL antibody [2H12] (AB270253)
  • WB

Supplier Data

Western blot - Anti-Bcl-XL antibody [2H12] (AB270253)

All lanes:

Western blot - Anti-Bcl-XL antibody [2H12] (ab270253) at 2 µg/mL

All lanes:

Jurkat cell lysate

Predicted band size: 26 kDa

false

不同偶联物与剂型 (2)

关键信息

宿主种属

Mouse

克隆

Monoclonal

克隆号

2H12

亚型

IgG2a

轻链类型

kappa

不含载体蛋白

No

反应种属

Human

应用

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human BCL2L1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q07817

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A/G
纯化说明
Purified from Bioreactor Concentrate.
存储溶液
pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Bcl-XL also known as Bcl-xL or Bcl-Xl protein is a member of the Bcl-2 protein family playing an important role in regulating cell death. Bcl-XL inhibits apoptosis by binding to pro-apoptotic proteins preventing the release of cytochrome c from mitochondria. This protein has a molecular weight of approximately 30 kDa. Bcl-xL is widely expressed in tissues notably in the brain endothelial cells and hematopoietic tissues where it contributes to cell survival.
Biological function summary

This protein acts as an anti-apoptotic molecule within the mitochondria and is part of the Bcl-2 family complex. Bcl-XL modulates the balance between pro-apoptotic and anti-apoptotic signals impacting cell fate. By binding and sequestering pro-apoptotic members such as Bax and Bak Bcl-XL prevents mitochondrial outer membrane permeabilization important for avoiding programmed cell death. This function not only supports cellular longevity but also understanding cancer cell survival.

Pathways

The Bcl-XL protein holds a significant position in the apoptotic signaling pathways. It is particularly involved in the intrinsic pathway where its interactions with mitochondrial proteins Bax and Bak determine cell survival or death. It is interconnected with the PI3K/AKT pathway where AKT kinase activity can upregulate Bcl-XL expression demonstrating how survival signals are transmitted. These interactions highlight its pivotal role in balancing life and death at the cellular level.

Bcl-XL's overexpression often connects to the progression of cancers and resistance to chemotherapy. By inhibiting programmed cell death Bcl-XL allows cancer cells to evade traditional treatments. Furthermore research implicates Bcl-XL in neurodegenerative disorders where its interaction with Bcl-2 proteins disrupts normal apoptotic processes contributing to cellular dysfunction and disease. Understanding Bcl-XL's disease-related roles furthers the development of targeted therapies combating its protective effects in pathological contexts.
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Potent inhibitor of cell death. Inhibits activation of caspases. Appears to regulate cell death by blocking the voltage-dependent anion channel (VDAC) by binding to it and preventing the release of the caspase activator, CYC1, from the mitochondrial membrane. Also acts as a regulator of G2 checkpoint and progression to cytokinesis during mitosis.. Isoform Bcl-X(L) also regulates presynaptic plasticity, including neurotransmitter release and recovery, number of axonal mitochondria as well as size and number of synaptic vesicle clusters. During synaptic stimulation, increases ATP availability from mitochondria through regulation of mitochondrial membrane ATP synthase F(1)F(0) activity and regulates endocytic vesicle retrieval in hippocampal neurons through association with DMN1L and stimulation of its GTPase activity in synaptic vesicles. May attenuate inflammation impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed : 17418785).. Isoform Bcl-X(S) promotes apoptosis.
See full target information BCL2L1
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文献 (3)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70606 PubMed40916543

2025

Neuroprotective Effects of Transcranial Pulsed Current Stimulation: Modulation of Microglial Polarization in Traumatic Brain Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Yao,Bingkai Ren,Qianhui Zhou,Yang Bai,Zhen Feng

Nature communications 12:4932 PubMed34389733

2021

The conformational stability of pro-apoptotic BAX is dictated by discrete residues of the protein core.

Applications

Unspecified application

Species

Unspecified reactive species

Noah B Bloch,Thomas E Wales,Michelle S Prew,Hannah R Levy,John R Engen,Loren D Walensky

Aging 13:19750-19759 PubMed34351305

2021

Dual inhibition of FGFR4 and BCL-xL inhibits multi-resistant ovarian cancer with BCL2L1 gain.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Guo,Chao Gu,Bin Li,Congjian Xu
View all publications
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替代产品

Primary Antibodies

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Primary Antibodies

AB223547

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Primary Antibodies

AB32370

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Primary Antibodies

AB178844

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搭配使用产品

Assay Kits

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Primary Antibodies

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Secondary Antibodies

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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

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Primary Antibodies

AB32064

Anti-Cleaved PARP1 antibody [E51]

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cleaved PARP1 antibody [E51] (AB32064)

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Primary Antibodies

AB32503

Anti-Bax antibody [E63]

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