Anti-Bcl-2抗体[Bcl2/100] (ab117115)
![Western blot - Anti-Bcl-2 antibody [Bcl2/100] (ab117115)](https://www.abcam.cn/ps/products/117/ab117115/Images/ab117115-275404-anti-bcl-2-antibody-bcl2-100-western-blot.jpg "Western blot - Anti-Bcl-2 antibody [Bcl2/100] (ab117115)")
Key features and details
- Mouse monoclonal [Bcl2/100] to Bcl-2
- Suitable for: WB, Flow Cyt (Intra)
- Knockout validated
- Reacts with: Human
- Isotype: IgG1
概述
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产品名称
Anti-Bcl-2抗体[Bcl2/100]
参阅全部 Bcl-2 一抗 -
描述
小鼠单克隆抗体[Bcl2/100] to Bcl-2 -
宿主
Mouse -
经测试应用
适用于: WB, Flow Cyt (Intra)more details -
种属反应性
与反应: Human
预测可用于: Cow, Cat, Pig
不与反应:
Mouse -
免疫原
Synthetic peptide within Human Bcl-2 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P10415 -
阳性对照
- WB: Wild type HAP1 whole cell lysate, HeLa, and THP-1 whole cell lysates. Flow Cyt (Intra): Human peripheral whole blood.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Protein A purified -
纯化说明
ab117115 is purified from cell culture supernatant by protein-A affinity chromatography. Purity is > 95% (by SDS-PAGE). -
克隆
单克隆 -
克隆编号
Bcl2/100 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab117115于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 26 kDa.
|
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| Flow Cyt (Intra) |
Use a concentration of 1 - 5 µg/ml.
|
| 说明 |
|---|
|
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 26 kDa. |
|
Flow Cyt (Intra)
Use a concentration of 1 - 5 µg/ml. |
靶标
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功能
Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1). May attenuate inflammation by impairing NLRP1-inflammasome activation, hence CASP1 activation and IL1B release (PubMed:17418785). -
组织特异性
Expressed in a variety of tissues. -
疾病相关
A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions. -
序列相似性
Belongs to the Bcl-2 family. -
结构域
BH1 and BH2 domains are required for the interaction with BAX and for anti-apoptotic activity.
The BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
The loop between motifs BH4 and BH3 is required for the interaction with NLRP1. -
翻译后修饰
Phosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
Monoubiquitinated by PARK2, leading to increase its stability. Ubiquitinated by SCF(FBXO10), leading to its degradation by the proteasome. -
细胞定位
Mitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane. - Information by UniProt
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数据库链接
- Entrez Gene: 281020 Cow
- Entrez Gene: 596 Human
- Omim: 151430 Human
- SwissProt: O02718 Cow
- SwissProt: P10415 Human
- Unigene: 150749 Human
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别名
- Apoptosis regulator Bcl 2 antibody
- Apoptosis regulator Bcl-2 antibody
- Apoptosis regulator Bcl2 antibody
see all
图片
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Western blot - Anti-Bcl-2 antibody [Bcl2/100] (ab117115)
Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: BCL2 knockout HAP1 whole cell lysate (20 µg)
Lane 3: HeLa whole cell lysate (20 µg)
Lane 4: THP-1 whole cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab117115 observed at 26 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab117115 was shown to specifically react with BCL2 when BCL2 knockout samples were used. Wild-type and BCL2 knockout samples were subjected to SDS-PAGE. Ab117115 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/10000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging. -
![Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [Bcl2/100] (ab117115)](https://www.abcam.cn/ps/products/117/ab117115/Images/ab117115-535928-anti-bcl-2-antibody-ccl2-100-flow-cytometry-intracellular-human-lymphocytes.jpg)
Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [Bcl2/100] (ab117115)Flow cytometric analysis of Human peripheral whole blood labeling Bcl-2 with ab117115 at 1 µg/mL, showing the separation of human lymphocytes (red-filled) from neutrophil granulocytes (black-dashed).
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![Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [Bcl2/100] (ab117115)](https://www.abcam.cn/ps/products/117/ab117115/Images/ab117115-535929-anti-bcl-2-antibody-ccl2-100-flow-cytometry-intracellular-human-peripheral-whole-blood.jpg)
Flow Cytometry (Intracellular) - Anti-Bcl-2 antibody [Bcl2/100] (ab117115)Flow cytometric analysis of Human peripheral whole blood labeling Bcl-2 with ab117115 at 1 µg/mL.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (54)
ab117115 被引用在 54 文献中.
- Shen HM et al. E2F1-mediated KDM4A-AS1 up-regulation promotes EMT of hepatocellular carcinoma cells by recruiting ILF3 to stabilize AURKA mRNA. Cancer Gene Ther 30:1007-1017 (2023). PubMed: 36973424
- Ge Y et al. Discovery of Salidroside as a Novel Non-Coding RNA Modulator to Delay Cellular Senescence and Promote BK-Dependent Apoptosis in Cerebrovascular Smooth Muscle Cells of Simulated Microgravity Rats. Int J Mol Sci 24:N/A (2023). PubMed: 37833978
- Lee JH & Kim SH Functional and morphological maturation of the full-sized and mini-pig corpus luteum by programmed cell death mechanism. J Vet Res 67:307-314 (2023). PubMed: 38143820
- Li L et al. CMTM5 inhibits the development of prostate cancer via the EGFR/PI3K/AKT signaling pathway. Mol Med Rep 25:N/A (2022). PubMed: 34791506
- Wang L et al. Negative regulation of miR-1288-3p/KRT4 axis through a circular RNA in oral cancer. J Biochem Mol Toxicol 36:e23118 (2022). PubMed: 35707935