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AB201225

Anti-BCKDHB 抗体

Anti-BCKDHB antibody

5

(3 Reviews)

|

(4 Publications)

Rabbit Polyclonal BCKDHB antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Recombinant Fragment Protein within Human 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial aa 150 to C-terminus.

查看别名

Branched-chain alpha-keto acid dehydrogenase E1 component beta chain, BCKDE1B, BCKDH E1-beta, BCKDHB

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHB antibody (AB201225)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHB antibody (AB201225)

Immunohistochemical analysis of paraffin embedded Human fetal stomach tissue labeling BCKDHB with ab201225 at 1/100 dilution.

Western blot - Anti-BCKDHB antibody (AB201225)
  • WB

Supplier Data

Western blot - Anti-BCKDHB antibody (AB201225)

All lanes:

Western blot - Anti-BCKDHB antibody (ab201225) at 1/500 dilution

Lane 1:

HeLa cell lysate

Lane 2:

293T cell lysate

Lane 3:

Jurkat cell lysate

Predicted band size: 43 kDa

true

关键信息

宿主种属

Rabbit

克隆

Polyclonal

亚型

IgG

不含载体蛋白

No

反应种属

Human

应用

IHC-P, WB

applications

免疫原

Recombinant Fragment Protein within Human 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P21953

反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

性能和储存信息

形式
Lyophilized
复溶
reconstitute with water at 200µL
纯化工艺
Affinity purification Immunogen
存储溶液
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
-20°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle

产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

靶点信息

Together with BCKDHA forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction.
See full target information 2-oxoisovalerate dehydrogenase subunit beta, mitochondrial

文献 (4)

Recent publications for all applications. Explore the full list and refine your search

Acta diabetologica 62:227-240 PubMed39150511

2024

Accumulation of branched-chain amino acids deteriorates the neuroinflammatory response of Müller cells in diabetic retinopathy via leucine/Sestrin2-mediated sensing of mTOR signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Qiaoyun Gong,Jingyi Wang,Dawei Luo,Yupeng Xu,Rulin Zhang,Xin Li,Zihan Yin,Junwei Fang,Haiyan Wang

Nature communications 13:3278 PubMed35672312

2022

Neonatal gene therapy achieves sustained disease rescue of maple syrup urine disease in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Clément Pontoizeau,Marcelo Simon-Sola,Clovis Gaborit,Vincent Nguyen,Irina Rotaru,Nolan Tual,Pasqualina Colella,Muriel Girard,Maria-Grazia Biferi,Jean-Baptiste Arnoux,Agnès Rötig,Chris Ottolenghi,Pascale de Lonlay,Federico Mingozzi,Marina Cavazzana,Manuel Schiff

Cell 183:269-283.e19 PubMed32916130

2020

A Quantitative Proteome Map of the Human Body.

Applications

Unspecified application

Species

Unspecified reactive species

Lihua Jiang,Meng Wang,Shin Lin,Ruiqi Jian,Xiao Li,Joanne Chan,Guanlan Dong,Huaying Fang,Aaron E Robinson,Michael P Snyder

Molecular genetics and metabolism 126:388-396 PubMed30709776

2019

Mild inborn errors of metabolism in commonly used inbred mouse strains.

Applications

Unspecified application

Species

Unspecified reactive species

João Leandro,Sara Violante,Carmen A Argmann,Jacob Hagen,Tetyana Dodatko,Aaron Bender,Wei Zhang,Evan G Williams,Alexis M Bachmann,Johan Auwerx,Chunli Yu,Sander M Houten
View all publications

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