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AB275961

重组Anti-BCKDHA (phospho S292)抗体[BLR060F]

Anti-BCKDHA (phospho S292) antibody [BLR060F]

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(1 Publication)

Rabbit Recombinant Monoclonal BCKDHA phospho S292 antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human BCKDHA phospho S292.

查看别名

Branched-chain alpha-keto acid dehydrogenase E1 component alpha chain, BCKDE1A, BCKDH E1-alpha, BCKDHA

3 Images
Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)
  • WB

Supplier Data

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

Chemiluminescence with an exposure time of 30 seconds.

Lower Panel : Rabbit anti-RPL10

All lanes:

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate

Lane 2:

TCMK-1 (mouse kidney epithelial cell line) whole cell lysate

Lane 3:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

Lane 5:

LNCaP (human prostate cancer cell line) whole cell lysate

Lane 6:

GaMG whole cell lysate

Lane 7:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

Lane 8:

MCF-7 (human breast adenocarcinoma cell line) whole cell lysate

Lane 9:

Differentiated 3T3-L1 whole cell lysate

Secondary

All lanes:

HRP-conjugated goat anti-rabbit IgG

Predicted band size: 50 kDa

false

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)
  • WB

Supplier Data

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

All lanes:

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/1000 dilution

Lane 1:

HEK293T cells mock treated (-) whole cell lysate at 50 µg

Lane 2:

HEK293T cells treated with phosphatases (+) whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 50 kDa

Observed band size: 49 kDa

true

Exposure time: 3min

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)
  • WB

Supplier Data

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (AB275961)

All lanes:

Western blot - Anti-BCKDHA (phospho S292) antibody [BLR060F] (ab275961) at 1/1000 dilution

Lane 1:

NIH 3T3 whole cell lysate at 50 µg

Lane 2:

CT26 whole cell lysate at 50 µg

Lane 3:

TCMK-1 whole cell lysate at 50 µg

Lane 4:

BW5147.3 whole cell lysate at 50 µg

Lane 5:

CH27 whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 50 kDa

Observed band size: 49 kDa

true

Exposure time: 75s

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

BLR060F

亚型

IgG

不含载体蛋白

No

反应种属

Mouse, Human

应用

WB

applications

免疫原

Synthetic Peptide within Human BCKDHA phospho S292. The exact immunogen used to generate this antibody is proprietary information.

P12694

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搭配使用产品

Primary Antibodies

AB126173

Anti-BCKDHA antibody

Immunocytochemistry/ Immunofluorescence - Anti-BCKDHA antibody (AB126173)

  • 0
  • 0
Primary Antibodies

AB128935

Anti-BCKDK antibody [EPR7564]

RabMAb|Recombinant|KO Validated

Western blot - Anti-BCKDK antibody [EPR7564] (AB128935)

  • 5
  • 1
Primary Antibodies

AB302504

Anti-BCKDHA (phospho S292) antibody [EPR27489-76]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA (phospho S292) antibody [EPR27489-76] (AB302504)

  • 0
  • 0
Reagents, Controls & Accessories

AB110413

Total OXPHOS Rodent WB Antibody Cocktail

Western blot - Total OXPHOS Rodent WB Antibody Cocktail (AB110413)

  • 5
  • 18
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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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产品详情

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

This product is sold under License from Bethyl Laboratories, Inc.

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性能和储存信息

形式
Liquid
纯化说明
Recombinant antibody was purified from cell culture supernatant.
存储溶液
pH: 7.8 - 8.6 Preservative: 0.09% Sodium azide Constituents: 99.81% Borate buffered saline, 0.1% BSA
运输条件
Blue Ice
推荐的短期储存时间
1-2 weeks
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
分装信息
Upon delivery aliquot
储存信息
Avoid freeze / thaw cycle
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

Together with BCKDHB forms the heterotetrameric E1 subunit of the mitochondrial branched-chain alpha-ketoacid dehydrogenase (BCKD) complex. The BCKD complex catalyzes the multi-step oxidative decarboxylation of alpha-ketoacids derived from the branched-chain amino-acids valine, leucine and isoleucine producing CO2 and acyl-CoA which is subsequently utilized to produce energy. The E1 subunit catalyzes the first step with the decarboxylation of the alpha-ketoacid forming an enzyme-product intermediate. A reductive acylation mediated by the lipoylamide cofactor of E2 extracts the acyl group from the E1 active site for the next step of the reaction.
See full target information BCKDHA phospho S292
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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Experimental physiology 108:961-974 PubMed37139700

2023

High doses of rosuvastatin induce impaired branched-chain amino acid catabolism and lead to insulin resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Xue Bai,Xingzhen Long,Fang Song,Baolin Chen,Changcheng Sheng,Cailin Tang,Li Li,Jiaxing Zhang,Rui Zhang,Jiquan Zhang,Jiali Li
View all publications
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Abcam Product Promise

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详情请参阅我们的条款与条件。

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