Anti-BCKDHA抗体(ab126173)
Key features and details
- Rabbit polyclonal to BCKDHA
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-BCKDHA抗体
参阅全部 BCKDHA 一抗 -
描述
兔多克隆抗体to BCKDHA -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 252-432 of Human BCKDHA (UniProt P12694).
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阳性对照
- HepG2 whole cell lysate (ab7900); HeLa cells; Human Colon cancer tissue; A549. WB: Wild-type HEK-293T cell lysate. Daudi cell lysate. Human heart cell lysate.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG
相关产品
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Compatible Secondaries
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab126173于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 50 kDa.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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| ICC/IF |
1/100 - 1/500.
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| 说明 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 50 kDa. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
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ICC/IF
1/100 - 1/500. |
靶标
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功能
The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). -
疾病相关
Maple syrup urine disease 1A -
序列相似性
Belongs to the BCKDHA family. -
细胞定位
Mitochondrion matrix. - Information by UniProt
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数据库链接
- Entrez Gene: 593 Human
- Omim: 608348 Human
- SwissProt: P12694 Human
- Unigene: 433307 Human
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别名
- Branched chain alpha keto acid dehydrogenase E1 component alpha polypeptide antibody
- FLJ45695 antibody
- OVD1A antibody
see all
图片
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Western blot - Anti-BCKDHA antibody (ab126173)All lanes : Anti-BCKDHA antibody (ab126173) at 1/500 dilution
Lane 1 : Wild-type HEK-293T cell lysate
Lane 2 : BCKDHA knockout HEK-293T cell lysate
Lane 3 : Human Heart cell lysate
Lane 4 : Daudi cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-BCKDHA antibody staining at 1/500 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab126173 was shown to bind specifically to BCKDHA. A band was observed at 48 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in BCKDHA knockout cell line ab266439 (knockout cell lysate ab258324). To generate this image, wild-type and BCKDHA knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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Western blot - Anti-BCKDHA antibody (ab126173)Anti-BCKDHA antibody (ab126173) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 50 kDa
10% SDS PAGE -
Immunocytochemistry/ Immunofluorescence - Anti-BCKDHA antibody (ab126173)ab126173, at 1/500 dilution, staining BCKDHA in methanol-fixed HeLa cells by Immunofluorescence. The lower panel is costained with Hoechst 33342. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCKDHA antibody (ab126173)ab126173, at 1/250 dilution, staining BCKDHA in paraffin-embedded Human Colon cancer tissue by Immunohistochemistry.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab126173 被引用在 3 文献中.
- Wu G et al. Exercise Enhances Branched-Chain Amino Acid Catabolism and Decreases Cardiac Vulnerability to Myocardial Ischemic Injury. Cells 11:N/A (2022). PubMed: 35626742
- Pontoizeau C et al. Neonatal gene therapy achieves sustained disease rescue of maple syrup urine disease in mice. Nat Commun 13:3278 (2022). PubMed: 35672312
- Hull J et al. Distribution of the branched-chain α-ketoacid dehydrogenase complex E1α subunit and glutamate dehydrogenase in the human brain and their role in neuro-metabolism. Neurochem Int 112:49-58 (2018). PubMed: 29104034