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Signal Transduction Metabolism Amino Acids

Anti-BCAT1抗体(ab125209)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (1)

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Western blot - Anti-BCAT1 antibody (ab125209)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT1 antibody (ab125209)

Key features and details

  • Rabbit polyclonal to BCAT1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

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概述

  • 产品名称

    Anti-BCAT1抗体
    参阅全部 BCAT1 一抗
  • 描述

    兔多克隆抗体to BCAT1
  • 宿主

    Rabbit
  • 经测试应用

    适用于: WB, IHC-Pmore details
  • 种属反应性

    与反应: Human
  • 免疫原

    Synthetic peptide from the C terminal region of Human BCAT1

  • 阳性对照

    • IHC-P: Human breast tissue. WB: HeLa and Jurkat cell lysates.
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • 存储溶液

    Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Signal Transduction
    • Metabolism
    • Amino Acids
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Amino acid metabolism
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human BCAT1 protein (ab124556)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab125209于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 43 kDa.
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
说明
WB
Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 43 kDa.
IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

靶标

  • 功能

    Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine.
  • 组织特异性

    During embryogenesis, expressed in the brain and kidney. Overexpressed in MYC-induced tumors such as Burkitt's lymphoma.
  • 序列相似性

    Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.
  • 细胞定位

    Cytoplasm.
  • Target information above from: UniProt accession P54687 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 586 Human
    • Omim: 113520 Human
    • SwissProt: P54687 Human
    • Unigene: 438993 Human
    • 别名

      • BCAT antibody
      • BCAT(c) antibody
      • Bcat1 antibody
      • BCAT1_HUMAN antibody
      • BCT1 antibody
      • Branched chain amino acid aminotransferase, cytosolic antibody
      • branched chain amino-acid transaminase 1, cytosolic antibody
      • branched chain aminotransferase 1 antibody
      • branched chain aminotransferase 1, cytosolic antibody
      • Branched-chain-amino-acid aminotransferase antibody
      • cytosolic antibody
      • DKFZp686E12175 antibody
      • EC 2.6.1.42 antibody
      • ECA39 antibody
      • MECA39 antibody
      • placental protein 18 antibody
      • PNAS 121 antibody
      • PP18 antibody
      • Protein ECA39 antibody
      see all

    图片

    • Western blot - Anti-BCAT1 antibody (ab125209)
      Western blot - Anti-BCAT1 antibody (ab125209)
      All lanes : Anti-BCAT1 antibody (ab125209) at 0.5 µg/ml

      Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
      Lane 2 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate

      Predicted band size: 43 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT1 antibody (ab125209)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BCAT1 antibody (ab125209)
      Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling BCAT1 with ab125209 at 0.5 μg/ml.

    实验方案

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (1)

    发表研究结果有使用 ab125209?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab125209 被引用在 1 文献中.

    • Belone Ade F  et al. Genome-Wide Screening of mRNA Expression in Leprosy Patients. Front Genet 6:334 (2015). IHC ; Human . PubMed: 26635870

    客户评价及客户问答

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    提交评价 提交问题

    1-2 of 2 Abreviews or Q&A

    Question

    Yes I would be interested in receiving this antibody as part of the testing program. We should also have tissue from a Bcat1 knockout rat in the next 6 months so that will help a lot with testing.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 04 2012

    Answer

    I am very pleased to hear you would like to accept our offer and testab125209 in rat. This code will give you: 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ratand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: https://www.abcam.com/collaborationdiscount.

    Read More

    Abcam Scientific Support

    回复于 Sep 04 2012

    Question

    I am looking for a Bcat1 antibody that will work for IHC-P in rat. I have searched extensively on the web and the closest I have found is your antibody (ab125209) that is supposed to work for IHC-P in human. Is there any indication that this antibody might cross-react with rat based on homology? Do you have any images from the human IHC-P tissue that I could see that support its compatibility with IHC-P?

    Read More

    Abcam community

    Verified customer

    Asked on Aug 30 2012

    Answer

    Thank you for contacting us. I have attached an image for IHC-P in human mammary cancer tissue. The homology between the immunogen and the rat tissue is 82%. Generally we predict reactivity at 85% or higher, so there is a definite chance you could be successful with this product.

    Read More

    Abcam Scientific Support

    回复于 Aug 30 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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