Anti-BBX抗体[2065C12a] (ab50824)
![Western blot - Anti-BBX antibody [2065C12a] (ab50824)](https://www.abcam.cn/ps/products/50/ab50824/Images/ab50824-62516-anti-bbx-antibody-2065c12a-western-blot.jpg "Western blot - Anti-BBX antibody [2065C12a] (ab50824)")
Key features and details
- Mouse monoclonal [2065C12a] to BBX
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG1
概述
-
产品名称
Anti-BBX抗体[2065C12a]
参阅全部 BBX 一抗 -
描述
小鼠单克隆抗体[2065C12a] to BBX -
宿主
Mouse -
经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Recombinant fragment (Human)
-
阳性对照
- HeLa cells or 3T3 cells nuclear or whole celle lysate
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Store at 4°C (stable for up to 12 months). Store at -20°C or -80°C. -
存储溶液
pH: 7.4
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading... -
纯度
Protein G purified -
纯化说明
ab50824 was purified from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filter sterilized. -
克隆
单克隆 -
克隆编号
2065C12a -
同种型
IgG1 -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab50824于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/50. Predicted molecular weight: 105 kDa.
|
|
| ICC/IF |
1/50.
|
| 说明 |
|---|
|
WB
1/50. Predicted molecular weight: 105 kDa. |
|
ICC/IF
1/50. |
靶标
-
相关性
Function: Transcription factor that is necessary for cell cycle progression from G1 to S phase. Similarity: Contains 1 HMG box DNA-binding domain. -
细胞定位
Nuclear -
数据库链接
- Entrez Gene: 56987 Human
- SwissProt: A2RRM7 Human
- SwissProt: Q8WY36 Human
- Unigene: 124366 Human
- Unigene: 736885 Human
-
别名
- BBX antibody
- BBX_HUMAN antibody
- Bobby sox homolog antibody
see all
图片
-
Western blot - Anti-BBX antibody [2065C12a] (ab50824)Anti-BBX antibody [2065C12a] (ab50824) at 1/50 dilution + HeLa whole cell lysate at 50 µg
Secondary
anti-mouse IgG at 1/2500 dilution
Predicted band size: 105 kDa
Observed band size: 130 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-BBX antibody [2065C12a] (ab50824)](https://www.abcam.cn/ps/products/50/ab50824/Images/ab50824-62515-anti-bbx-antibody-2065c12a-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-BBX antibody [2065C12a] (ab50824)Immunofluorescent staining of BBX expression HeLa cells using ab50824 at a 1/50 dilution and and AlexaFluor 488 conjugated goat anti-mouse secondary
实验方案
数据表及文件
-
Datasheet download
文献 (0)
ab50824 尚未被引用在任何文献中。
