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重组RabMAb

重组Anti-BBS2抗体[EPR13183] (ab181134)

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Western blot - Anti-BBS2 antibody [EPR13183] (ab181134)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] (ab181134)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] (ab181134)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] (ab181134)
  • Anti-BBS2 antibody [EPR13183] (ab181134)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13183] to BBS2
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

概述

  • 产品名称

    Anti-BBS2抗体[EPR13183]
    参阅全部 BBS2 一抗

  • 描述

    兔单克隆抗体[EPR13183] to BBS2

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • NTERA2/D1, NCCIT, MCF-7 and HeLa whole cell lysate (ab150035); Human endometrial carcinoma, retina and thyroid gland tissues.

  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • 纯度

    Tissue culture supernatant

  • 克隆

    单克隆

  • 克隆编号

    EPR13183

  • 同种型

    IgG

  • 研究领域

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab181134于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
说明
WB
1/1000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 相关性

    Defects in BBS2 are the cause of Bardet-Biedl syndrome type 2. Bardet-Biedl syndrome is a genetically heterogeneous, autosomal recessive disorder characterized by usually severe pigmentary retinopathy, early onset obesity, polydactyly, hypogenitalism, renal malformation and mental retardation.

  • 细胞定位

    Cytoplasmic

  • 数据库链接

    • 别名

      • Bardet Biedl syndrome 2 antibody
      • Bardet Biedl syndrome 2 protein antibody
      • BBS antibody
      • MGC20703 antibody

    图片

    • Western blot - Anti-BBS2 antibody [EPR13183] (ab181134)
      Western blot - Anti-BBS2 antibody [EPR13183] (ab181134)
      All lanes : Anti-BBS2 antibody [EPR13183] (ab181134) at 1/10000 dilution

      Lane 1 : NTERA2/D1 cell lysate
      Lane 2 : NCCIT cell lysate
      Lane 3 : MCF-7 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

      Predicted band size: 80 kDa
      Observed band size: 80 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] (ab181134)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] (ab181134)

      Immunohistochemical analysis of paraffin-embedded Human endometrial carcinoma tissue labeling BBS2 with ab181134 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] (ab181134)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] (ab181134)

      Immunohistochemical analysis of paraffin-embedded Human retina tissue labeling BBS2 with ab181134 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] (ab181134)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BBS2 antibody [EPR13183] (ab181134)

      Immunohistochemical analysis of paraffin-embedded Human thyroid gland tissue labeling BBS2 with ab181134 at 1/100 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-BBS2 antibody [EPR13183] (ab181134)
      Anti-BBS2 antibody [EPR13183] (ab181134)

    文献 (0)

    发表研究结果有使用 ab181134？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab181134 尚未被引用在任何文献中。

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