AB289364

重组Anti-Bax抗体[HL236] - BSA and Azide free

Anti-Bax antibody [HL236] - BSA and Azide free

Recombinant
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(2 Publications)

Rabbit Recombinant Monoclonal BAX antibody. Carrier free. Suitable for IHC-P, WB and reacts with Rat, Mouse, Chinese hamster, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human BAX.

查看别名

BCL2L4, BAX, Apoptosis regulator BAX, Bcl-2-like protein 4, Bcl2-L-4

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Immunohistochemical analysis of paraffin-embedded rat tissues labelling Bax protein with ab289364 at a 1/100 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue staining for Bax using ab289364 diluted at 1 : 50.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 mins.

Supplier Data

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Samples were separated by 12% SDS-PAGE.

All lanes:

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (ab289364) at 1/1000 dilution

All lanes:

Raw264.7 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21 kDa

false

Supplier Data

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Samples were separated by 15% SDS-PAGE.

All lanes:

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (ab289364) at 1/1000 dilution

Lane 1:

Wild-type (WT) CHO whole cell lysates at 30 µg

Lane 2:

BAX knockout (KO) CHO whole cell lysates at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 21 kDa

false

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

HL236

亚型

IgG

不含载体蛋白

Yes

反应种属

Rat, Mouse, Human

应用

WB, IHC-P

applications

免疫原

Synthetic Peptide within Human BAX. The exact immunogen used to generate this antibody is proprietary information.

Q07812

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A

存储溶液

pH: 7.4 Constituents: PBS

运输条件

Blue Ice

补充信息

This supplementary information is collated from multiple sources and compiled automatically.

Bax also known as Bcl-2-associated X protein plays an important role in the regulation of apoptosis. It is widely known for its pro-apoptotic function opposing the action of anti-apoptotic proteins like Bcl-2. Bax has a molecular weight of approximately 21 kDa making it identifiable in laboratory techniques such as western blot. Researchers often use anti-Bax antibodies to detect and analyze its expression levels in various studies. The Bax protein is mainly expressed in mitochondria where it undergoes changes in response to apoptotic signals.

Biological function summary

The Bax protein forms homodimers or heterodimers with other Bcl-2 family proteins to facilitate apoptosis. This protein is part of a larger complex involving several members of the Bcl-2 family which finely tunes the balance between cell survival and programmed cell death. Through a change in its conformation Bax translocates to the outer mitochondrial membrane where it promotes the release of cytochrome c and other apoptogenic factors into the cytosol.

Pathways

Bax plays a significant role in the intrinsic pathway of apoptosis also known as the mitochondrial pathway. This pathway involves the release of cytochrome c which further activates downstream proteins such as caspase-9 and caspase-3. Bax interacts closely with proteins like Bak another pro-apoptotic member of the Bcl-2 family providing a cooperative function in mitochondrial membrane permeabilization. Together Bax and Bak act as gatekeepers of the mitochondrial pathway determining the cell's fate in response to apoptotic stimuli.

The dysregulation of Bax often associates with various cancers and neurodegenerative diseases. In cancer reduced Bax expression or function can lead to resistance to apoptosis contributing to unchecked cellular proliferation. Conversely in neurodegenerative disorders such as Alzheimer's disease increased Bax activity results in excessive neuronal apoptosis. The balance between Bax and its partner proteins like Bcl-2 or Bcl-xL is important in maintaining normal cellular function and preventing disease progression.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Plays a role in the mitochondrial apoptotic process (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 36361894, PubMed : 8358790, PubMed : 8521816). Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM) (PubMed : 21458670). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816). Promotes activation of CASP3, and thereby apoptosis (PubMed : 10772918, PubMed : 11060313, PubMed : 16113678, PubMed : 16199525, PubMed : 18948948, PubMed : 21199865, PubMed : 21458670, PubMed : 25609812, PubMed : 8358790, PubMed : 8521816).

See full target information BAX

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文献 (2)

Recent publications for all applications. Explore the full list and refine your search

Aging 15:12264-12274 PubMed37934569

2023

Influences and mechanism of erythropoietin on the cognitive function of vascular dementia rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhipeng Tang,Xiuqin Li,Nan Yin,Ming Zhao,Qingchuan Hu,Peiyuan Lv

Bioengineered 13:14426-14437 PubMed36694458

2023

Long non-coding RNA lincRNA-erythroid prosurvival attenuates inflammation by enhancing myosin heavy chain 6 stability through recruitment of heterogeneous nuclear ribonucleoprotein L in myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Huizhen Zhang,Xuejun Kou,Dong Xiao,Zhangjian Yu

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

重组Anti-Bax抗体[HL236] - BSA and Azide free

Anti-Bax antibody [HL236] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

反应性数据

产品详情

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (2)

Abcam Product Promise

重组Anti-Bax抗体[HL236] - BSA and Azide free

Anti-Bax antibody [HL236] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

Western blot - Anti-Bax antibody [HL236] - BSA and Azide free (AB289364)

关键信息

宿主种属

克隆

克隆号

亚型

不含载体蛋白

反应种属

应用

免疫原

搭配使用产品

Assay Kits

Primary Antibodies

Primary Antibodies

Secondary Antibodies

Primary Antibodies

反应性数据

产品详情

性能和储存信息

形式

纯化工艺

存储溶液

运输条件

推荐的短期储存条件

推荐的长期储存条件

补充信息

Biological function summary

Pathways

产品实验方案

靶点信息

文献 (2)

Abcam Product Promise