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Cell Biology Apoptosis Intracellular Associated Proteins
重组

重组Anti-Bax抗体[5B7] (ab3191)

  • Datasheet
  • SDS
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Western blot - Anti-Bax antibody [5B7] (ab3191)
  • Flow Cytometry (Intracellular) - Anti-Bax antibody [5B7] (ab3191)
  • Immunoprecipitation - Anti-Bax antibody [5B7] (ab3191)
  • Anti-Bax antibody [5B7] (ab3191)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [5B7] to Bax
  • Suitable for: Flow Cyt (Intra), WB, IP
  • Reacts with: Mouse

Conjugates logo Related conjugates and formulations

Carrier Free

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概述

  • 产品名称

    Anti-Bax抗体[5B7]
    参阅全部 Bax 一抗
  • 描述

    小鼠单克隆抗体[5B7] to Bax
  • 宿主

    Mouse
  • 经测试应用

    适用于: Flow Cyt (Intra), WB, IPmore details
  • 种属反应性

    与反应: Mouse
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • WB: A20 and C2C12 lysate. Flow Cyt (intra): RAW 264.7 cells.
  • 常规说明

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 0.5% BSA, PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    单克隆
  • 克隆编号

    5B7
  • 同种型

    IgG1
  • 轻链类型

    kappa
  • 研究领域

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Associated Proteins
    • Cell Biology
    • Apoptosis
    • Intracellular
    • Bcl2 Family
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Bcl 2 family
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Apoptosis marker and proteins ELISA kits
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Bcl 2 family
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

相关产品

  • Alternative Versions

    • Anti-Bax antibody [5B7] - BSA and Azide free (ab252258)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab3191于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
Flow Cyt (Intra)
Use a concentration of 1.043 µg/ml.
WB
Use a concentration of 0.417 µg/ml. Predicted molecular weight: 21 kDa.
IP
Use a concentration of 13.9 µg/ml.
说明
Flow Cyt (Intra)
Use a concentration of 1.043 µg/ml.
WB
Use a concentration of 0.417 µg/ml. Predicted molecular weight: 21 kDa.
IP
Use a concentration of 13.9 µg/ml.

靶标

  • 功能

    Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
  • 组织特异性

    Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro-myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T-cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
  • 序列相似性

    Belongs to the Bcl-2 family.
  • 结构域

    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.
  • 细胞定位

    Cytoplasm and Mitochondrion membrane. Cytoplasm. Colocalizes with 14-3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane.
  • Target information above from: UniProt accession Q07812 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 12028 Mouse
    • SwissProt: Q07813 Mouse
    • Unigene: 19904 Mouse
    • 别名

      • Apoptosis regulator BAX antibody
      • BAX antibody
      • Bax-protein antibody
      • BAX_HUMAN antibody
      • BAXA antibody
      • Baxdelta2G9 antibody
      • Baxdelta2G9omega antibody
      • Baxdelta2omega antibody
      • Bcl-2-like protein 4 antibody
      • BCL2 associated X protein antibody
      • BCL2 associated X protein omega antibody
      • BCL2 associated X protein transcript variant delta2 antibody
      • Bcl2-L-4 antibody
      • BCL2L4 antibody
      • membrane isoform alpha antibody
      see all

    图片

    • Western blot - Anti-Bax antibody [5B7] (ab3191)
      Western blot - Anti-Bax antibody [5B7] (ab3191)
      Lane 1 : Anti-Bax antibody [5B7] (ab3191) at 0.417 mg/ml
      Lane 2 : Anti-Bax antibody [5B7] (ab3191) at 0.417 µg/ml

      Lane 1 : A20 (Mouse reticulum sarcoma B lymphocyte) lysate
      Lane 2 : C2C12 (Mouse myoblasts) lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 21 kDa


      Exposure time: 26 seconds


      Blocking/Dilution buffer: 5% NFDM/TBST.

    • Flow Cytometry (Intracellular) - Anti-Bax antibody [5B7] (ab3191)
      Flow Cytometry (Intracellular) - Anti-Bax antibody [5B7] (ab3191)

      Intracellular flow cytometry analysis of 4% paraformaldehyde-fixed, 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling Bax with ab3191 at a 1.043µg/ml  (red) compared to a mouse monoclonal IgG isotype control (black) and cells without incubation with primary antibody and secondary antibody (blue). Goat anti-mouse IgG (Alexa Fluor® 488, ab150113) was used as the secondary at a 1/2000 dilution.

       

    • Immunoprecipitation - Anti-Bax antibody [5B7] (ab3191)
      Immunoprecipitation - Anti-Bax antibody [5B7] (ab3191)

      Bax was immunoprecipitated from RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate with ab190908 at 13.9µg/ml. Western blot was performed from the immunoprecipitate using ab3191 at 1/1000 dilution. Anti-mouse IgG for IP (HRP) (ab131368) was used as secondary antibody at 1/50000 dilution.
      Lane 1: RAW 264.7 whole cell lysate 10ug (Input).
      Lane 2: ab3191 IP in RAW 264.7 whole cell lysate.
      Lane 3: Mouse monoclonal IgG instead of ab3191 in RAW 264.7 whole cell lysate.
      Blocking/Dilution buffer: 5% NFDM/TBST.
      Exposure time: 30 seconds.

    • Anti-Bax antibody [5B7] (ab3191)
      Anti-Bax antibody [5B7] (ab3191)

    实验方案

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (10)

    发表研究结果有使用 ab3191?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab3191 被引用在 10 文献中.

    • Yu L  et al. Blockage of AMPK-ULK1 pathway mediated autophagy promotes cell apoptosis to increase doxorubicin sensitivity in breast cancer (BC) cells: an in vitro study. BMC Cancer 21:195 (2021). PubMed: 33632157
    • Xiao J  et al. Circular RNA CSNK1G1 promotes the progression of osteoarthritis by targeting the miR-4428/FUT2 axis. Int J Mol Med 47:232-242 (2021). PubMed: 33416120
    • Wang M  et al. Integrated study of miR-215 promoting breast cancer cell apoptosis by targeting RAD54B. J Cell Mol Med 25:3327-3338 (2021). PubMed: 33635591
    • Duan N  et al. Overexpression of HIPK2 removes the transrepression of proapoptotic genes mediated by the CtBP1-p300-FOXO3a complex and increases the chemosensitivity in osteosarcoma cells. J Cancer 12:1826-1837 (2021). PubMed: 33613771
    • McKelvey KJ  et al. Glycolysis and Fatty Acid Oxidation Inhibition Improves Survival in Glioblastoma. Front Oncol 11:633210 (2021). PubMed: 33854970
    • Thongchot S  et al. Cancer-Associated Fibroblast-Derived IL-6 Determines Unfavorable Prognosis in Cholangiocarcinoma by Affecting Autophagy-Associated Chemoresponse. Cancers (Basel) 13:N/A (2021). PubMed: 33925189
    • Liu Q  et al. Brucine inhibits proliferation of glioblastoma cells by targeting the G-quadruplexes in the c-Myb promoter. J Cancer 12:1990-1999 (2021). PubMed: 33753997
    • Lu S & Lin CW Lentivirus-mediated transfer of gene encoding fibroblast growth factor-18 inhibits intervertebral disc degeneration. Exp Ther Med 22:856 (2021). PubMed: 34178129
    • Cui X  et al. miR-132 is upregulated in polycystic ovarian syndrome and inhibits granulosa cells viability by targeting Foxa1. Mol Med Rep 22:5155-5162 (2020). PubMed: 33174054
    • Shi X  et al. Persimmon oligomeric proanthocyanidins alleviate ultraviolet B-induced skin damage by regulating oxidative stress and inflammatory responses. Free Radic Res 54:765-776 (2020). PubMed: 33108915

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