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AB317330

重组Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein抗体[abd166] - BSA and Azide free

Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] - BSA and Azide free

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Human Recombinant Monoclonal Bat SHC014-CoV RBD antibody. Carrier free. Suitable for I-ELISA, Dot and reacts with Recombinant fragment - Bat SARS-like coronavirus RsSHC014, Transfected cell lysate - Bat SARS-like coronavirus RsSHC014 samples.
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Indirect ELISA - Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] - BSA and Azide free (AB317330)
  • I-ELISA

Supplier Data

Indirect ELISA - Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] - BSA and Azide free (AB317330)

This data was developed using ab317329, the same antibody clone in a different buffer formulation.

Indirect ELISA analysis of ab317329 at 2000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Human IgG (H+L) at 1 : 1000 dilution.

Antigen : bat SARS-like coronavirus RsSHC014 Spike glycoprotein,SARS-CoV-2 (2019-nCoV) Spike glycoprotein,MERS-CoV Spike glycoprotein,HCoV-HKU1 Spike glycoprotein.

Antigen concentration : 1000 ng/ml

Dot Blot - Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] - BSA and Azide free (AB317330)
  • Dot

Supplier Data

Dot Blot - Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] - BSA and Azide free (AB317330)

This data was developed using ab317329, the same antibody clone in a different buffer formulation.

Dot blot analysis of bat SARS-like coronavirus RsSHC014 Spike glycoprotein using ab317329 at 1 : 1000 followed by a Goat Anti-Human IgG Fc (HRP) preadsorbed (ab98624) at 1 : 10,000 dilution.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Dot Blot - Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166] (<a href='/products/primary-antibodies/bat-sars-like-coronavirus-rsshc014-spike-glycoprotein-antibody-abd166-ab317329'>ab317329</a>) at 1/1000 dilution

Lane 1:

293T cells transfected with an empty vector containing a myc-his tag whole cell lysate

Lane 2:

293T cells transfected with a Bat Spike glycoprotein expression vector containing a myc-his-tag whole cell lysate

Lane 3:

His-tagged bat SARS-like coronavirus RsSHC01 recombinant fragment protein

Secondary

All lanes:

Dot Blot - Goat Anti-Human IgG Fc (HRP) preadsorbed (<a href='/products/secondary-antibodies/goat-human-igg-fc-hrp-preadsorbed-ab98624'>ab98624</a>) at 1/10000 dilution

false

Exposure time: 180s

不同偶联物与剂型 (1)

  • Unconjugated

    Anti-bat SARS-like coronavirus RsSHC014 Spike glycoprotein antibody [abd166]

关键信息

宿主种属

Human

克隆

Monoclonal

克隆号

abd166

亚型

IgG1

不含载体蛋白

Yes

反应种属

Bat SARS-like coronavirus RsSHC014

应用

I-ELISA, Dot

applications

免疫原

The exact immunogen used to generate this antibody is proprietary information.

特异性

This antibody does not cross-react with SARS-CoV-2 Spike glycoprotein, MERS-CoV Spike glycoprotein and HCoV-HKU1 Spike glycoprotein.

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Bat SARS-like coronavirus RsSHC014": { "IELISA-species-checked": "predicted", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "predicted", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant fragment - Bat SARS-like coronavirus RsSHC014": { "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "<p></p>", "Dot-species-checked": "notRecommended", "Dot-species-dilution-info": "", "Dot-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Transfected cell lysate - Bat SARS-like coronavirus RsSHC014": { "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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产品详情

ab317330 is the carrier-free version of ab317329.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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性能和储存信息

形式
Liquid
纯化工艺
Affinity purification Protein A
存储溶液
pH: 7.2 - 7.4 Constituents: PBS
运输条件
Blue Ice
推荐的短期储存条件
+4°C
推荐的长期储存条件
+4°C
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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:
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靶点信息

See full target information Bat SHC014-CoV RBD
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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。
详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com