Anti-BAP31抗体(ab37120)
Key features and details
- Rabbit polyclonal to BAP31
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
概述
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产品名称
Anti-BAP31抗体
参阅全部 BAP31 一抗 -
描述
兔多克隆抗体to BAP31 -
宿主
Rabbit -
经测试应用
适用于: IHC-P, WBmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to 17 amino acids near to the C terminus of the human BAP31 protein.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab37120于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| IHC-P |
Use a concentration of 2 µg/ml.
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| WB | (1) |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa).
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| 说明 |
|---|
|
IHC-P
Use a concentration of 2 µg/ml. |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 31 kDa (predicted molecular weight: 28 kDa). |
靶标
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功能
May play a role in anterograde transport of membrane proteins from the endoplasmic reticulum to the Golgi. May be involved in CASP8-mediated apoptosis. -
组织特异性
Ubiquitous. -
疾病相关
Note=BCAP31 is deleted in the chromosome Xq28 deletion syndrome which involves BCAP31 and the and the promoter region of ABCD1. -
序列相似性
Belongs to the BCAP29/BCAP31 family. -
翻译后修饰
Cleaved by CASP8 and other caspases. -
细胞定位
Endoplasmic reticulum membrane. Endoplasmic reticulum-Golgi intermediate compartment membrane. May shuttle between the ER and the intermediate compartment/cis-Golgi complex. - Information by UniProt
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数据库链接
- Entrez Gene: 10134 Human
- Entrez Gene: 27061 Mouse
- Entrez Gene: 293852 Rat
- Omim: 300398 Human
- SwissProt: P51572 Human
- SwissProt: Q61335 Mouse
- Unigene: 522817 Human
- Unigene: 17 Mouse
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别名
- 6C6 AG antibody
- 6C6 AG tumor associated antigen antibody
- 6C6-AG tumor-associated antigen antibody
see all
图片
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Western blot - Anti-BAP31 antibody (ab37120)All lanes : Anti-BAP31 antibody (ab37120) at 0.5 µg/ml
Lane 1 : 293 cell lysate
Lane 2 : A431 cell lysates
Lane 3 : A559 cell lysate
Lane 4 : Caco-2 (Human colorectal adenocarcinoma) cell lysate
Lane 5 : Daudi cell lysate
Lane 6 : HeLa cell lysate
Lane 7 : HepG2 cell lysate
Lane 8 : K562 cell lysate
Lane 9 : MCF-7 cell lysate
Lane 10 : Jurkat cell lysate
Lane 11 : SK-N-SH cell lysate
Lane 12 : THP-1 cell lysate
Lane 13 : 3T3/NIH
Lane 14 : YB2/0 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat anti-rabbit IgG HRP conjugate at 1/10000 dilution
Predicted band size: 28 kDa -
Western blot - Anti-BAP31 antibody (ab37120)Lane 1 : Anti-BAP31 antibody (ab37120) at 0.5 µg/ml
Lane 2 : Anti-BAP31 antibody (ab37120) at 1 µg/ml
Lane 3 : Anti-BAP31 antibody (ab37120) at 2 µg/ml
All lanes : Ramos cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 28 kDa
Observed band size: 31 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BAP31 antibody (ab37120)ab37120 at 2µg/ml staining BAP31 in rat lung tissue by IHC
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab37120 被引用在 7 文献中.
- Ma C et al. Low expression of B-Cell-Associated protein 31 is associated with unfavorable prognosis in human colorectal cancer. Pathol Res Pract 214:661-666 (2018). PubMed: 29653744
- Kim IY et al. Loperamide overcomes the resistance of colon cancer cells to bortezomib by inducing CHOP-mediated paraptosis-like cell death. Biochem Pharmacol N/A:N/A (2018). PubMed: 30529689
- Tan N et al. Low expression of B-cell-associated protein 31 in human primary hepatocellular carcinoma correlates with poor prognosis. Histopathology 68:221-9 (2016). PubMed: 25980696
- Li Y et al. Interaction between human BAP31 and respiratory syncytial virus small hydrophobic (SH) protein. Virology 482:105-10 (2015). PubMed: 25854864
- Galindo I et al. The ATF6 branch of unfolded protein response and apoptosis are activated to promote African swine fever virus infection. Cell Death Dis 3:e341 (2012). WB . PubMed: 22764100
- Panaretakis T et al. Mechanisms of pre-apoptotic calreticulin exposure in immunogenic cell death. EMBO J 28:578-90 (2009). WB ; Mouse . PubMed: 19165151
- Gross AJ et al. Developmental acquisition of the Lyn-CD22-SHP-1 inhibitory pathway promotes B cell tolerance. J Immunol 182:5382-92 (2009). WB ; Mouse . PubMed: 19380785