重组Anti-BANF1/BAF抗体[EPR7669] (ab129074)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7669] to BANF1/BAF
- Suitable for: Flow Cyt (Intra), WB, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
概述
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产品名称
Anti-BANF1/BAF抗体[EPR7669]
参阅全部 BANF1/BAF 一抗 -
描述
兔单克隆抗体[EPR7669] to BANF1/BAF -
宿主
Rabbit -
经测试应用
适用于: Flow Cyt (Intra), WB, ICC/IFmore details
不适用于: IHC-P -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human BANF1/BAF aa 50-150. The exact sequence is proprietary.
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阳性对照
- HeLa, HepG2 and 293T cell lysate; Human thyroid gland carcinoma tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR7669 -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
- Anti-BANF1/BAF antibody [EPR7669] - BSA and Azide free (ab248281)
- Alexa Fluor® 488 Anti-BANF1/BAF antibody [EPR7669] (ab320416)
- Alexa Fluor® 647 Anti-BANF1/BAF antibody [EPR7669] (ab320417)
- Alexa Fluor® 555 Anti-BANF1/BAF antibody [EPR7669] (ab320418)
- Alexa Fluor® 594 Anti-BANF1/BAF antibody [EPR7669] (ab320419)
- PE Anti-BANF1/BAF antibody [EPR7669] (ab320420)
- APC Anti-BANF1/BAF antibody [EPR7669] (ab320421)
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab129074于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
---|---|---|
Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
|
WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa).
|
ICC/IF |
1/50.
|
说明 |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 10 kDa (predicted molecular weight: 10 kDa). |
ICC/IF
1/50. |
靶标
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功能
Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging. Exploited by retroviruses for inhibiting self-destructing autointegration of retroviral DNA, thereby promoting integration of viral DNA into the host chromosome. EMD and BAF are cooperative cofactors of HIV-1 infection. Association of EMD with the viral DNA requires the presence of BAF and viral integrase. The association of viral DNA with chromatin requires the presence of BAF and EMD. -
组织特异性
Widely expressed. Expressed in colon, brain, heart, kidney, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine, spleen and testis. Not detected in thymus and peripheral blood leukocytes. -
序列相似性
Belongs to the BAF family. -
结构域
Has a helix-hairpin-helix (HhH) structural motif conserved among proteins that bind non-specifically to DNA.
LEM domain proteins bind centrally on the BAF dimer, whereas DNA binds to the left and right sides. -
翻译后修饰
Partially phosphorylated on serine. Ser-4 phosphorylation may block BAF ability to promote EMD binding to lamins in vitro. Non phosphorylated BAF seems to enhances binding between EMD and LMNA. -
细胞定位
Nucleus. Cytoplasm. Chromosome. Significantly enriched at the nuclear inner membrane, diffusely throughout the nucleus during interphase and concentrated at the chromosomes during the M-phase. May be included in HIV-1 virions via its interaction with viral GAG polyprotein. - Information by UniProt
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数据库链接
- Entrez Gene: 8815 Human
- Entrez Gene: 23825 Mouse
- Entrez Gene: 114087 Rat
- Omim: 603811 Human
- SwissProt: O75531 Human
- SwissProt: O54962 Mouse
- SwissProt: Q9R1T1 Rat
- Unigene: 433759 Human
see all -
别名
- BAF antibody
- BAF_HUMAN antibody
- BANF 1 antibody
see all
图片
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All lanes : Anti-BANF1/BAF antibody [EPR7669] (ab129074) at 1/1000 dilution (Purified)
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate
Lane 3 : PC-12 (Rat adrenal gland pheochromocytoma ) whole cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 10 kDa -
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling BANF1/BAF with Purified ab129074 at 1:20 dilution (10 ?g/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1:2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling BANF1/BAF with Purified ab129074 at 1:50 dilution (6.0 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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