重组Anti-Band 3/AE 1抗体[EPR1425(2)] (ab175214)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1425(2)] (ab175214)](https://www.abcam.cn/ps/products/175/ab175214/Images/ab175214-201507-anti-band-3-ae-1-antibody-epr14252-immunohistochemistry.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1425(2)] (ab175214)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1425(2)] to Band 3/AE 1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-Band 3/AE 1抗体[EPR1425(2)]
参阅全部 Band 3/AE 1 一抗 -
描述
兔单克隆抗体[EPR1425(2)] to Band 3/AE 1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details
不适用于: ICC/IF or IP -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- Human red blood cell lysate; Human lung and spleen tissue.
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常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR1425(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab175214于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
1/100000 - 1/500000. Predicted molecular weight: 100 kDa.
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| IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| 说明 |
|---|
|
WB
1/100000 - 1/500000. Predicted molecular weight: 100 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Functions both as a transporter that mediates electroneutral anion exchange across the cell membrane and as a structural protein. Major integral membrane glycoprotein of the erythrocyte membrane; required for normal flexibility and stability of the erythrocyte membrane and for normal erythrocyte shape via the interactions of its cytoplasmic domain with cytoskeletal proteins, glycolytic enzymes, and hemoglobin. Functions as a transporter that mediates the 1:1 exchange of inorganic anions across the erythrocyte membrane. Mediates chloride-bicarbonate exchange in the kidney, and is required for normal acidification of the urine. -
组织特异性
Detected in erythrocytes (at protein level) (PubMed:7506871, PubMed:26542571). Isoform 2 is expressed in kidney (at protein level) (PubMed:7506871). -
疾病相关
Ovalocytosis, Southeast Asian
Spherocytosis 4
Renal tubular acidosis, distal, autosomal dominant
Renal tubular acidosis, distal, with hemolytic anemia
Renal tubular acidosis, distal, with normal red cell morphology
Cryohydrocytosis -
序列相似性
Belongs to the anion exchanger (TC 2.A.31) family. -
翻译后修饰
Phosphorylated on Tyr-8 and Tyr-21 most likely by SYK. PP1-resistant phosphorylation that precedes Tyr-359 and Tyr-904 phosphorylation.
Phosphorylated on Tyr-359 and Tyr-904 most likely by LYN. PP1-inhibited phosphorylation that follows Tyr-8 and Tyr-21 phosphorylation.
N-glycosylated. -
细胞定位
Cell membrane. Basolateral cell membrane. Detected in the erythrocyte cell membrane and on the basolateral membrane of alpha-intercalated cells in the collecting duct in the kidney. - Information by UniProt
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数据库链接
- Entrez Gene: 6521 Human
- Omim: 109270 Human
- SwissProt: P02730 Human
- Unigene: 210751 Human
- Unigene: 443948 Human
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别名
- AE 1 antibody
- AE1 antibody
- Anion exchange protein 1 antibody
see all
图片
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1425(2)] (ab175214)
Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Band 3/AE 1 at 1/250 dilution. Note erythrocyte staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1425(2)] (ab175214)](https://www.abcam.cn/ps/products/175/ab175214/Images/ab175214-201504-anti-band-3-ae-1-antibody-epr14252-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Band 3/AE 1 antibody [EPR1425(2)] (ab175214)Immunohistochemical analysis of paraffin-embedded Human lung tissue labeling Band 3/AE 1 wtih ab175214 at 1/250 dilution. Note erythrocyte staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Western blot - Anti-Band 3/AE 1 antibody [EPR1425(2)] (ab175214)](https://www.abcam.cn/ps/products/175/ab175214/Images/ab175214-201503-anti-band-3-ae-1-antibody-epr14252-western-blot.jpg)
Western blot - Anti-Band 3/AE 1 antibody [EPR1425(2)] (ab175214)Anti-Band 3/AE 1 antibody [EPR1425(2)] (ab175214) at 1/100000 dilution + Human red blood cell lysate at 10 µg
Predicted band size: 100 kDa -
![Anti-Band 3/AE 1 antibody [EPR1425(2)] (ab175214)](https://www.abcam.cn/ps/products/175/ab175214/Images/ab175214-4-benefits-of-recombinant-antibodies.png)
Anti-Band 3/AE 1 antibody [EPR1425(2)] (ab175214)
数据表及文件
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SDS download
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Datasheet download
文献 (0)
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