Anti-Bak抗体(ab69404)
Key features and details
- Rabbit polyclonal to Bak
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
-
产品名称
Anti-Bak抗体
参阅全部 Bak 一抗 -
描述
兔多克隆抗体to Bak -
宿主
Rabbit -
经测试应用
适用于: WB, ICC/IF, IHC-Pmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide derived from the sequence of human Bak, conjugated to KLH.
-
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
-
形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab69404于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | (1) |
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
|
| ICC/IF |
Use at an assay dependent concentration.
|
|
| IHC-P |
1/50.
|
| 说明 |
|---|
|
WB
1/1000. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa). |
|
ICC/IF
Use at an assay dependent concentration. |
|
IHC-P
1/50. |
靶标
-
功能
In the presence of an appropriate stimulus, accelerates programmed cell death by binding to, and antagonizing the anti-apoptotic action of BCL2 or its adenovirus homolog E1B 19k protein. Low micromolar levels of zinc ions inhibit the promotion of apoptosis. -
组织特异性
Expressed in a wide variety of tissues, with highest levels in the heart and skeletal muscle. -
序列相似性
Belongs to the Bcl-2 family. -
结构域
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
细胞定位
Mitochondrion membrane. - Information by UniProt
-
数据库链接
- Entrez Gene: 578 Human
- Omim: 600516 Human
- SwissProt: Q16611 Human
- Unigene: 485139 Human
-
别名
- Apoptosis regulator BAK antibody
- BAK antibody
- BAK like antibody
see all
图片
-
Western blot - Anti-Bak antibody (ab69404)Anti-Bak antibody (ab69404) at 1/1000 dilution + A431 cell lysate
Predicted band size: 23 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Bak antibody (ab69404)Immunofluorescent staining of HeLa cells using ab69404. nuclei were counter-stained using DRAQ5. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bak antibody (ab69404)Immunohistochemical analysis of human cervical cancer tissue using 1/50 ab69404.
实验方案
数据表及文件
-
SDS download
-
Datasheet download
文献 (5)
ab69404 被引用在 5 文献中.
- Quinlan S et al. Canine Papillomavirus 2 E6 Does Not Interfere With UVB-Induced Upregulation of p53 and p53-Regulated Genes. Front Vet Sci 8:570982 (2021). PubMed: 33748203
- Feng Q et al. Intracellular expression of arginine deiminase activates the mitochondrial apoptosis pathway by inhibiting cytosolic ferritin and inducing chromatin autophagy. BMC Cancer 20:665 (2020). PubMed: 32677906
- Yuan C et al. BNIP3/Bcl-2-mediated apoptosis induced by cyclic tensile stretch in human cartilage endplate-derived stem cells. Exp Ther Med 15:235-241 (2018). PubMed: 29375685
- Li Z et al. Inhibition of neuroblastoma proliferation by PF-3758309, a small-molecule inhibitor that targets p21-activated kinase 4. Oncol Rep 38:2705-2716 (2017). PubMed: 29048629
- Chen G et al. Suppression of Sirt1 sensitizes lung cancer cells to WEE1 inhibitor MK-1775-induced DNA damage and apoptosis. Oncogene 36:6863-6872 (2017). PubMed: 28869605