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Anti-Bad (phospho S155)抗体(ab28825)

Reviews (1)Q&A (1)References (5)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad (phospho S155) antibody (ab28825)
  • Western blot - Anti-Bad (phospho S155) antibody (ab28825)

Key features and details

  • Rabbit polyclonal to Bad (phospho S155)
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

概述

  • 产品名称

    Anti-Bad (phospho S155)抗体
    参阅全部 Bad 一抗

  • 描述

    兔多克隆抗体to Bad (phospho S155)

  • 宿主

    Rabbit

  • 经测试应用

    适用于: WB, IHC-Pmore details

  • 种属反应性

    与反应: Human

  • 免疫原

    Synthetic peptide corresponding to Bad (phospho S155).

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab28825于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (1)
1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 18 kDa).
IHC-P
Use at an assay dependent concentration.
说明
WB
1/500 - 1/1000. Detects a band of approximately 23 kDa (predicted molecular weight: 18 kDa).
IHC-P
Use at an assay dependent concentration.

靶标

  • 功能

    Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

  • 组织特异性

    Expressed in a wide variety of tissues.

  • 序列相似性

    Belongs to the Bcl-2 family.

  • 结构域

    Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family.

  • 翻译后修饰

    Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2.

  • 细胞定位

    Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm.
  • Information by UniProt

  • 数据库链接

    • 别名

      • AI325008 antibody
      • BAD antibody
      • BAD_HUMAN antibody
      • BBC 2 antibody
      • BBC2 antibody
      • BBC6 antibody
      • Bcl 2 Antagonist of Cell Death antibody
      • Bcl 2 Binding Component 6 antibody
      • BCL X / BCL 2 Binding Protein antibody
      • BCL X Binding Protein antibody
      • Bcl XL/Bcl 2 Associated Death Promoter antibody
      • Bcl-2-binding component 6 antibody
      • Bcl-2-like protein 8 antibody
      • Bcl-XL/Bcl-2-associated death promoter antibody
      • Bcl2 antagonist of cell death antibody
      • BCL2 antagonist of cell death protein antibody
      • BCL2 associated agonist of cell death antibody
      • Bcl2 Associated Death Promoter antibody
      • BCL2 binding component 6 antibody
      • BCL2 binding protein antibody
      • Bcl2 Like 8 Protein antibody
      • Bcl2-L-8 antibody
      • BCL2L8 antibody
      • Proapoptotic BH3 Only Protein antibody
      see all

    图片

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad (phospho S155) antibody (ab28825)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bad (phospho S155) antibody (ab28825)
      Ab28825, at a concentration of 1/50, staining Bad in paraffin embedded human breast carcinoma tissue by Immunohistochemistry.
      Left image: untreated.
      Right image: treated with synthesized phosphopeptide.
    • Western blot - Anti-Bad (phospho S155) antibody (ab28825)
      Western blot - Anti-Bad (phospho S155) antibody (ab28825)
      Anti-Bad (phospho S155) antibody (ab28825) at 1/500 dilution + extracts from 293 cells (5-30ug) treated with forsklin

      Predicted band size: 18 kDa
      Observed band size: 23 kDa why is the actual band size different from the predicted?

    文献 (5)

    发表研究结果有使用 ab28825？请让我们知道，以便我们可以引用本数据表中的参考文章。

    ab28825 被引用在 5 文献中.

    • Boac BM  et al. Expression of the BAD pathway is a marker of triple-negative status and poor outcome. Sci Rep 9:17496 (2019). PubMed: 31767884
    • Ke J  et al. Edaravone reduces oxidative stress and intestinal cell apoptosis after burn through up-regulating miR-320 expression. Mol Med 25:54 (2019). PubMed: 31829167
    • Zhao L  et al. Protein kinase A determines platelet life span and survival by regulating apoptosis. J Clin Invest 127:4338-4351 (2017). PubMed: 29083324
    • Rong F  et al. Knockdown of RhoGDIα induces apoptosis and increases lung cancer cell chemosensitivity to paclitaxel. Neoplasma 59:541-50 (2012). PubMed: 22668020
    • Danial NN  et al. Dual role of proapoptotic BAD in insulin secretion and beta cell survival. Nat Med 14:144-53 (2008). PubMed: 18223655

    客户评价及客户问答

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    1-2 of 2 Abreviews or Q&A

    Western blot abreview for Anti-Bad (phospho S155) antibody

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Heart)
    Gel Running Conditions
    Reduced Denaturing (12% acrylamide)
    Loading amount
    45 µg
    Specification
    Heart
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 2.5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    提交于 Jul 22 2015

    Question

    Thanks for your help with this.

    Read More
    Answer

    Thank you for contacting us.

    Your credit note ID is 19867.

    I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice of a future order by passing it on to your purchasing department. To avoid confusion, please ensure your accounts department is aware of how the credit note is being used. If you have questions on how to use the credit note, please contact our accounting department.

    Our accounting department can be contacted by email at us.credits@abcam.com or by telephone using the information at the Contact Us link in the top right corner of our website. Please refer to the credit note ID in any correspondence with our accounting department.

    The credit note ID is for your reference only and does not automatically guarantee the credit.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require further expert advice.

    Read More

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