AB278558

重组Anti-Bad (phospho S112)抗体[BADS112-B9]

Anti-Bad (phospho S112) antibody [BADS112-B9]

20ul selling size
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal BAD phospho S112 antibody. Suitable for Flow Cyt and reacts with Mouse, Rat, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human BAD pS112.

查看别名

BBC6, BCL2L8, BAD, Bcl2-associated agonist of cell death, Bcl-2-binding component 6, Bcl-2-like protein 8, Bcl-xL/Bcl-2-associated death promoter, Bcl2 antagonist of cell death, Bcl2-L-8

4 Images

Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (AB278558)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (AB278558)

Peptide blocking flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or Calyculin A-treated (green) or CalA and blocked with phospho peptide (gold) or CalA and blocked with non-phospho peptide (purple) using ab278558 at 0.1 µg/mL.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (AB278558)

Flow cytometric analysis of U937 cells secondary antibody only negative control (blue) or untreated (red) or treated with Calyculin A (green) using ab278558 at 0.01μg/mL.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (AB278558)

Flow cytometric analysis of C6 cells secondary antibody only negative control (blue), or treated with imatinib (red) or with pervanadate (green) using ab278558 at 0.08μg/mL.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-Bad (phospho S112) antibody [BADS112-B9] (AB278558)

ab278558 recognizes basal phosphorylation levels in mouse cells. Flow cytometric analysis of NIH3T3 cells secondary antibody only (blue) or 0.1 µg/mL of isotype control (orange) or of ab278558 (green).

不同偶联物与剂型 (2)

660 APC

APC Anti-Bad (phospho S112) antibody [BADS112-B9]
578 PE

PE Anti-Bad (phospho S112) antibody [BADS112-B9]

关键信息

宿主种属

Rabbit

克隆

Monoclonal

克隆号

BADS112-B9

亚型

IgG

轻链类型

lambda

不含载体蛋白

反应种属

Mouse, Rat, Human

应用

Flow Cyt

applications

免疫原

Synthetic Peptide within Human BAD pS112. The exact immunogen used to generate this antibody is proprietary information.

Q92934

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反应性数据

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "0.001-1 µg/mL", "FlowCyt-species-notes": "" } } }

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产品详情

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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性能和储存信息

形式

Liquid

纯化工艺

Affinity purification Protein A/G

存储溶液

Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.1% BSA

运输条件

Blue Ice

分装信息

Upon delivery aliquot

储存信息

Avoid freeze / thaw cycle

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补充信息

This supplementary information is collated from multiple sources and compiled automatically.

The Bad protein also known as Bcl-2-associated death promoter plays a critical role in apoptosis regulation. It possesses a molecular weight of approximately 23 kDa. Bad is expressed widely in tissues and is a member of the Bcl-2 family of proteins. This family is well-known for its involvement in the regulation of cell death both promoting and inhibiting depending on the protein’s interactions.

Biological function summary

Its main role is to promote apoptosis by binding to anti-apoptotic proteins like Bcl-2 and Bcl-XL displacing pro-apoptotic Bax and Bak proteins to trigger cell death. Bad functions as part of the mitochondrial apoptosis pathway and is known to form complexes with phosphorylated proteins. Phosphorylation of Bad by kinases can lead to its sequestration to the cytoplasm reducing apoptosis.

Pathways

Its action is pivotal in the PI3K/Akt signaling pathway. This pathway involves growth factors and cell survival signals where phosphorylation of Bad inhibits its pro-apoptotic activity. Bad interacts with proteins like Akt and 14-3-3 to modulate cell survival. Disruption in its regulation through these pathways may lead to abnormal cell survival or death.

Bad has implications in cancer and neurodegenerative diseases. Cancer cells often exhibit impaired apoptotic pathways where overexpression of Bad can ameliorate responsiveness to therapy. In neurodegenerative diseases such as Alzheimer's dysregulation of its function can contribute to cell death. The protein’s interaction with Bcl-2 and Bcl-XL is significant in these contexts influencing disease propagation and severity.

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产品实验方案

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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靶点信息

Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways.

See full target information BAD pS112

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文献 (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of experimental & clinical cancer research : CR 43:68 PubMed38439082

2024

MUC20 regulated by extrachromosomal circular DNA attenuates proteasome inhibitor resistance of multiple myeloma by modulating cuproptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobin Wang,Yingqing Shi,Hua Shi,Xiaoyu Liu,Aijun Liao,Zhuogang Liu,Robert Z Orlowski,Rui Zhang,Huihan Wang

View all publications

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Abcam Product Promise

我们致力于为您的研究提供高质量的试剂，为您科研的每一步提供支持。若我们的产品未能达到预期性能，我们向您提供 Abcam Product Promise 保障。

详情请参阅我们的条款与条件。

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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重组Anti-Bad (phospho S112)抗体[BADS112-B9]