Anti-Bad抗体(ab62465)
Key features and details
- Rabbit polyclonal to Bad
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术，可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Bad抗体
参阅全部 Bad 一抗 -
描述
兔多克隆抗体to Bad -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human -
免疫原
A 15 amino acid synthetic peptide from near the C terminal of human Bad
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阳性对照
- T24 cell lysate
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. -
存储溶液
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
纯度
Ion Exchange Chromatography -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab62465于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 18 kDa).
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| 说明 |
|---|
|
WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 18 kDa). |
靶标
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功能
Promotes cell death. Successfully competes for the binding to Bcl-X(L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X(L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways. -
组织特异性
Expressed in a wide variety of tissues. -
序列相似性
Belongs to the Bcl-2 family. -
结构域
Intact BH3 motif is required by BIK, BID, BAK, BAD and BAX for their pro-apoptotic activity and for their interaction with anti-apoptotic members of the Bcl-2 family. -
翻译后修饰
Phosphorylated on one or more of Ser-75, Ser-99, Ser-118 and Ser-134 in response to survival stimuli, which blocks its pro-apoptotic activity. Phosphorylation on Ser-99 or Ser-75 promotes heterodimerization with 14-3-3 proteins. This interaction then facilitates the phosphorylation at Ser-118, a site within the BH3 motif, leading to the release of Bcl-X(L) and the promotion of cell survival. Ser-99 is the major site of AKT/PKB phosphorylation, Ser-118 the major site of protein kinase A (CAPK) phosphorylation. Ser-75 is phosphorylated by AKT/PKB, protein kinase A and PIM2. -
细胞定位
Mitochondrion outer membrane. Cytoplasm. Upon phosphorylation, locates to the cytoplasm. - Information by UniProt
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数据库链接
- Entrez Gene: 572 Human
- Omim: 603167 Human
- SwissProt: Q92934 Human
- Unigene: 370254 Human
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别名
- AI325008 antibody
- BAD antibody
- BAD_HUMAN antibody
see all
图片
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Western blot - Anti-Bad antibody (ab62465)Lane 1 : Anti-Bad antibody (ab62465) at 0.5 µg/ml
Lane 2 : Anti-Bad antibody (ab62465) at 1 µg/ml
Lane 3 : Anti-Bad antibody (ab62465) at 2 µg/ml
All lanes : T24 cell lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Anti rabbit IgG secondary antibody
Predicted band size: 18 kDa
Observed band size: 22 kDa why is the actual band size different from the predicted?
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (10)
ab62465 被引用在 10 文献中.
- Choi YS et al. Chloroform extract of Citrus unshiu Markovich peel induces apoptosis and inhibits stemness in HeLa human cervical cancer cells. Mol Med Rep 23:N/A (2021). PubMed: 33236129
- Liang R et al. The Stress-Inducible BCL2A1 Is Required for Ovarian Cancer Metastatic Progression in the Peritoneal Microenvironment. Cancers (Basel) 13:N/A (2021). PubMed: 34572804
- Castro-Martinez F et al. Rictor/Mammalian Target of Rapamycin Complex 2 Signaling Protects Colonocytes from Apoptosis and Prevents Epithelial Barrier Breakdown. Am J Pathol 191:1537-1549 (2021). PubMed: 34139193
- Wäster P et al. Extracellular vesicles released by melanocytes after UVA irradiation promote intercellular signaling via miR21. Pigment Cell Melanoma Res 33:542-555 (2020). PubMed: 31909885
- Liu Q et al. circRNA 001306 enhances hepatocellular carcinoma growth by up-regulating CDK16 expression via sponging miR-584-5p. J Cell Mol Med 24:14306-14315 (2020). PubMed: 33135290
- Yen JH et al. Activation of dynamin-related protein 1 - dependent mitochondria fragmentation and suppression of osteosarcoma by cryptotanshinone. J Exp Clin Cancer Res 38:42 (2019). PubMed: 30691497
- Yang X et al. Nonylphenol promotes the proliferation of colorectal cancer COLO205 cells by upregulating the expression of protein kinase C ?. Oncol Lett 17:2498-2506 (2019). PubMed: 30675313
- Kabir S et al. The CUL5 ubiquitin ligase complex mediates resistance to CDK9 and MCL1 inhibitors in lung cancer cells. Elife 8:N/A (2019). PubMed: 31294695
- Singh A et al. Imprinting of cerebral cytochrome P450s in offsprings prenatally exposed to cypermethrin augments toxicity on rechallenge. Sci Rep 6:37426 (2016). WB ; Rat . PubMed: 27853314
- Wang H et al. Diallyl trisulfide induces osteosarcoma cell apoptosis through reactive oxygen species-mediated downregulation of the PI3K/Akt pathway. Oncol Rep 35:3648-58 (2016). PubMed: 27035545